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Team:IIT Madras/Notebook/Sandbox
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Ramakrishna (Talk | contribs) (→Intermediates and final constructs planned for the the 2 plasmid locking system) |
Ramakrishna (Talk | contribs) (→Intermediates planned through PCR protocol) |
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| + | {{:Team:IITM/main}} | ||
| - | + | <html> | |
| + | <br><br><br><br><br><br><br><br><br><br><br> | ||
| + | <br><br> | ||
| + | <head><style> | ||
| - | == Original design == | + | body |
| + | { | ||
| + | background: #000; | ||
| + | } | ||
| + | |||
| + | #aContent | ||
| + | { | ||
| + | background-color:#fff; | ||
| + | margin: 50px auto; | ||
| + | padding: 10px 10px 60px 10px; | ||
| + | width: 800px; | ||
| + | color: #000; | ||
| + | } | ||
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| + | </style> | ||
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| + | <STYLE TYPE="text/css"> | ||
| + | <!-- | ||
| + | .justifyalign {text-align:justify} | ||
| + | --> | ||
| + | </STYLE> | ||
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| + | </head> | ||
| + | |||
| + | <body> | ||
| + | |||
| + | <div id="aContent"> | ||
| + | </html> | ||
| + | |||
| + | ==Intermediates and proposed constructs for the the 2 plasmid locking system== | ||
| + | |||
| + | ==Original design== | ||
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker). | In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker). | ||
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<html><br></html> | <html><br></html> | ||
| - | + | <b><font color="#000">Plasmid 1</font></b> | |
<html><br></html> | <html><br></html> | ||
| + | |||
| + | [[Image:2plasmidoriginaldesignplasmid1.jpg]] | ||
| + | |||
<html><br></html> | <html><br></html> | ||
| + | <b><font color="#000">Plasmid 2</font></b> | ||
| - | == Final design == | + | <html><br></html> |
| + | |||
| + | [[Image:2plasmidoriginaldesignplasmid2.jpg]] | ||
| + | |||
| + | <html><br></html> | ||
| + | |||
| + | ==Final design== | ||
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this | We decided to remove the extra antibiotic markers from the inserts. Then it looks like this | ||
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<html><br></html> | <html><br></html> | ||
| - | + | <b><font color="#000">Plasmid 1</font></b> | |
<html><br></html> | <html><br></html> | ||
| - | We had more than one | + | We had more than one way to build plasmid 1 |
| + | <html><br></html> | ||
| - | = | + | <b><font color="#000">Path 1</font></b> |
| - | [[Image: | + | [[Image:2plasmidfinaldesignplasmid1path1.jpg]] |
<html><br></html> | <html><br></html> | ||
| + | <b><font color="#000">Path 1</font></b> | ||
| - | + | [[Image:2plasmidfinaldesignplasmid1path2.jpg]] | |
| - | + | <html><br></html> | |
| + | |||
| + | <b><font color="#000">Plasmid 2</font></b> | ||
<html><br></html> | <html><br></html> | ||
| - | + | [[Image:2plasmidfinaldesignplasmid2.jpg]] | |
| + | <html><br></html> | ||
| + | |||
| + | ==Intermediates and proposed constructs for the the 3 plasmid locking system== | ||
| + | |||
| + | We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too. | ||
| + | |||
| + | == Intermediates planned through PCR protocol == | ||
| + | |||
| + | <html><br></html> | ||
| - | + | These parts are essentially same as the those that occur in the construction strategies that are shown above. | |
| - | + | {{:Team:IITM/footer}} | |
Latest revision as of 01:30, 22 October 2009
IIT Madras
Contents |
Intermediates and proposed constructs for the the 2 plasmid locking system
Original design
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).
Plasmid 1
Plasmid 2
Final design
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this
Plasmid 1
We had more than one way to build plasmid 1
Path 1
Path 1
Plasmid 2
Intermediates and proposed constructs for the the 3 plasmid locking system
We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.
Intermediates planned through PCR protocol
These parts are essentially same as the those that occur in the construction strategies that are shown above.
