Team:IIT Madras/Notebook/Sandbox
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Ramakrishna (Talk | contribs) (→Original design) |
Ramakrishna (Talk | contribs) (→Intermediates planned through PCR protocol) |
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- | == Original design == | + | <html> |
+ | <br><br><br><br><br><br><br><br><br><br><br> | ||
+ | <br><br> | ||
+ | <head><style> | ||
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+ | body | ||
+ | { | ||
+ | background: #000; | ||
+ | } | ||
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+ | #aContent | ||
+ | { | ||
+ | background-color:#fff; | ||
+ | margin: 50px auto; | ||
+ | padding: 10px 10px 60px 10px; | ||
+ | width: 800px; | ||
+ | color: #000; | ||
+ | } | ||
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+ | </style> | ||
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+ | <STYLE TYPE="text/css"> | ||
+ | <!-- | ||
+ | .justifyalign {text-align:justify} | ||
+ | --> | ||
+ | </STYLE> | ||
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+ | </head> | ||
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+ | <body> | ||
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+ | <div id="aContent"> | ||
+ | </html> | ||
+ | |||
+ | ==Intermediates and proposed constructs for the the 2 plasmid locking system== | ||
+ | |||
+ | ==Original design== | ||
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker). | In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker). | ||
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<html><br></html> | <html><br></html> | ||
- | Plasmid 1 | + | <b><font color="#000">Plasmid 1</font></b> |
<html><br></html> | <html><br></html> | ||
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- | == Final design == | + | <b><font color="#000">Plasmid 2</font></b> |
+ | |||
+ | <html><br></html> | ||
+ | |||
+ | [[Image:2plasmidoriginaldesignplasmid2.jpg]] | ||
+ | |||
+ | <html><br></html> | ||
+ | |||
+ | ==Final design== | ||
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this | We decided to remove the extra antibiotic markers from the inserts. Then it looks like this | ||
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<html><br></html> | <html><br></html> | ||
- | + | <b><font color="#000">Plasmid 1</font></b> | |
<html><br></html> | <html><br></html> | ||
- | We had more than one | + | We had more than one way to build plasmid 1 |
+ | <html><br></html> | ||
- | = | + | <b><font color="#000">Path 1</font></b> |
- | [[Image: | + | [[Image:2plasmidfinaldesignplasmid1path1.jpg]] |
<html><br></html> | <html><br></html> | ||
+ | <b><font color="#000">Path 1</font></b> | ||
- | + | [[Image:2plasmidfinaldesignplasmid1path2.jpg]] | |
- | + | <html><br></html> | |
+ | |||
+ | <b><font color="#000">Plasmid 2</font></b> | ||
<html><br></html> | <html><br></html> | ||
- | == Intermediates and proposed constructs for the the 3 plasmid locking system == | + | [[Image:2plasmidfinaldesignplasmid2.jpg]] |
+ | |||
+ | <html><br></html> | ||
+ | |||
+ | ==Intermediates and proposed constructs for the the 3 plasmid locking system== | ||
We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too. | We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too. | ||
- | == | + | == Intermediates planned through PCR protocol == |
+ | |||
+ | <html><br></html> | ||
+ | |||
+ | These parts are essentially same as the those that occur in the construction strategies that are shown above. | ||
- | + | {{:Team:IITM/footer}} |
Latest revision as of 01:30, 22 October 2009
IIT Madras
Contents |
Intermediates and proposed constructs for the the 2 plasmid locking system
Original design
In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).
Plasmid 1
Plasmid 2
Final design
We decided to remove the extra antibiotic markers from the inserts. Then it looks like this
Plasmid 1
We had more than one way to build plasmid 1
Path 1
Path 1
Plasmid 2
Intermediates and proposed constructs for the the 3 plasmid locking system
We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.
Intermediates planned through PCR protocol
These parts are essentially same as the those that occur in the construction strategies that are shown above.