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Team:UCSF
From 2009.igem.org
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<!--- The Mission, Experiments ---> | <!--- The Mission, Experiments ---> | ||
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| - | <p align="center"><img src="https://static.igem.org/mediawiki/ | + | <p align="center"><img src="https://static.igem.org/mediawiki/2009/7/7e/Wiki_2009CellBots.jpg" width="276" height="258" align="middle" /></p> |
| - | <p align="center" class="style2"> | + | <br> |
| + | <br> | ||
| + | <p align="center" class="style2">Engineering Motile Cellular Robots</p> | ||
<blockquote> | <blockquote> | ||
<br> | <br> | ||
| - | + | <h2 align="left">Abstract</h2> | |
| - | + | <p align="left">Some eukaryotic cells, such as white blood cells, have the amazing ability to sense specific external chemical signals and move toward those signals. This behavior, known as chemotaxis, is a fundamental biological process crucial to such diverse functions as development, wound healing and immune response. In our project, we used a synthetic biology approach to manipulate signaling pathways that mediate chemotaxis in two model organisms:<br> HL-60 (neutrophil-like) cells and the slime mold, Dictyostelium discoideum. </p> | |
| - | <h2 align="left"> | + | |
| - | <p align="left">Some eukaryotic cells, such as white blood cells, have the amazing | + | <p align="left">In doing so, <strong>we have demonstrated that we can regulate both the navigation and speed of our cells, as well as harness their ability to carry a payload.</strong></p> |
| - | ability to sense specific external chemical signals | + | |
| - | those signals. This behavior, known as chemotaxis, is a fundamental | + | |
| - | biological process crucial to such diverse functions as development, | + | |
| - | wound healing and immune response. | + | |
| - | synthetic biology approach to manipulate signaling pathways that mediate | + | |
| - | chemotaxis in two model organisms: HL-60 (neutrophil-like) cells and the | + | |
| - | slime mold, Dictyostelium discoideum. | + | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | Through our manipulations, we hope to better understand how these | + | <p align="left">Through our manipulations, we hope to better understand how these systems work, and eventually to build or reprogram cells that can perform useful tasks. Imagine, for example, therapeutic nanorobots that could home to a directed site in the body and execute complex, user-defined functions (e.g., kill tumors, deliver drugs, guide stem cell migration and differentiation). Alternatively, imagine bioremediation nanorobots that could find and retrieve toxic substances. Such cellular robots could be revolutionary biotechnological tools.</p> |
| - | systems work, and eventually to build or reprogram cells that can | + | <p align="right"><a href="https://2009.igem.org/Team:UCSF/Project">More...</a></p> |
| - | perform | + | |
| - | could home to a directed site in the body and execute complex, | + | |
| - | user-defined functions (e.g., kill tumors, deliver drugs, guide stem | + | |
| - | cell migration and differentiation). Alternatively, imagine | + | |
| - | bioremediation nanorobots that could find and retrieve toxic | + | |
| - | substances. Such cellular robots could be revolutionary | + | |
| - | biotechnological tools.</p> | + | |
| - | <p align="right"><a href="https:// | + | |
<p align="right"> </p> | <p align="right"> </p> | ||
<table width="870" border="0" cellpadding="3"> | <table width="870" border="0" cellpadding="3"> | ||
<tr> | <tr> | ||
| - | <td width="202" height="208"><img src="https://static.igem.org/mediawiki/ | + | <td width="202" height="208"><img src="https://static.igem.org/mediawiki/2009/0/02/Wiki_2009project.jpg" alt="" width="240" height="224" /></td> |
<td width="650"><h3> </h3> | <td width="650"><h3> </h3> | ||
<blockquote> | <blockquote> | ||
| - | <h3> | + | <h3>BUILDING CELL-BOTS</h3> |
<blockquote> | <blockquote> | ||
| - | <h4><a href="https:// | + | <h4><a href="https://2009.igem.org/Team:UCSF/Project">Introduction</a></h4> |
| + | <h4><a href="https://2009.igem.org/Team:UCSF/Navigation">Step 1 - Engineering NAVIGATION</a></h4> | ||
<ul> | <ul> | ||
<ul> | <ul> | ||
| - | <li><a href="https:// | + | <li><a href="https://2009.igem.org/Team:UCSF/Navigation">Inserting New Sensors</a></li> |
| - | <li><a href="https:// | + | <li><a href="https://2009.igem.org/Team:UCSF/NavigationPart2">Tuning Sensor Sensitivity</a></li> |
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</ul> | </ul> | ||
</ul> | </ul> | ||
| - | <h4><a href="https:// | + | <h4><a href="https://2009.igem.org/Team:UCSF/SPEED">Step 2 - Engineering SPEED</a></h4> |
| - | <h4><a href="https:// | + | <h4><a href="https://2009.igem.org/Team:UCSF/PAYLOAD">Step 3 - Carrying a PAYLOAD</a></h4> |
| + | <h4><a href="https://2009.igem.org/Team:UCSF/Future Applications">Our Vision for the Future</a></h4> | ||
</blockquote> | </blockquote> | ||
</blockquote> | </blockquote> | ||
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<table width="870" border="0" cellpadding="3"> | <table width="870" border="0" cellpadding="3"> | ||
<tr> | <tr> | ||
| - | <td width="202" height="208"><img src="https://static.igem.org/mediawiki/ | + | <td width="202" height="208"><img src="https://static.igem.org/mediawiki/2009/d/db/Wiki_2009Team.jpg" alt="" width="240" height="224" /></td> |
<td width="650"><h3> </h3> | <td width="650"><h3> </h3> | ||
<blockquote> | <blockquote> | ||
<h3>OUR TEAM</h3> | <h3>OUR TEAM</h3> | ||
<blockquote> | <blockquote> | ||
| - | <h4><a href="https:// | + | <h4><a href="https://2009.igem.org/Team:UCSF/Team">Team Members</a></h4> |
| - | <h4><a href="https:// | + | <h4><a href="https://2009.igem.org/Team:UCSF/Notebook">Notebooks</a></h4> |
| - | <h4><a href="https:// | + | <h4><a href="https://2009.igem.org/Team:UCSF/Our_summer_experience">Summer Experience</a></h4> |
| - | <h4><a href="https:// | + | <h4><a href="https://2009.igem.org/Team:UCSF/Human Practices">Human Practices</a></h4> |
| - | <h4><a href=" | + | <h4><a href="http://dspace.mit.edu/handle/1721.1/46721">NEW BIOBRICK Standard RFC28 - Aar1 Cloning System</a></h4> |
| - | <h4><a href="https:// | + | <h4><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=UCSF">Parts submitted to the Registry</a></h4> |
| + | <h4><a href="https://2009.igem.org/Team:UCSF/Gold Medal Requisites">GOLD MEDAL Requisites</a></h4> | ||
</blockquote> | </blockquote> | ||
</blockquote> | </blockquote> | ||
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| - | '''UCSF iGEM | + | '''UCSF iGEM 2009 is sponsored by...''' |
<html> | <html> | ||
| - | <p align="center"><img src="https://static.igem.org/mediawiki/ | + | <p align="center"><img src="https://static.igem.org/mediawiki/2009/8/85/Wiki_2009Sponsors.jpg" width="690" height="419" align="middle" /></p></html> |
Latest revision as of 01:42, 22 October 2009
Engineering Motile Cellular Robots
Abstract
Some eukaryotic cells, such as white blood cells, have the amazing ability to sense specific external chemical signals and move toward those signals. This behavior, known as chemotaxis, is a fundamental biological process crucial to such diverse functions as development, wound healing and immune response. In our project, we used a synthetic biology approach to manipulate signaling pathways that mediate chemotaxis in two model organisms:
HL-60 (neutrophil-like) cells and the slime mold, Dictyostelium discoideum.In doing so, we have demonstrated that we can regulate both the navigation and speed of our cells, as well as harness their ability to carry a payload.
Through our manipulations, we hope to better understand how these systems work, and eventually to build or reprogram cells that can perform useful tasks. Imagine, for example, therapeutic nanorobots that could home to a directed site in the body and execute complex, user-defined functions (e.g., kill tumors, deliver drugs, guide stem cell migration and differentiation). Alternatively, imagine bioremediation nanorobots that could find and retrieve toxic substances. Such cellular robots could be revolutionary biotechnological tools.
BUILDING CELL-BOTS
Introduction
Step 1 - Engineering NAVIGATION
Step 2 - Engineering SPEED
Step 3 - Carrying a PAYLOAD
Our Vision for the Future
OUR TEAM
Team Members
Notebooks
Summer Experience
Human Practices
NEW BIOBRICK Standard RFC28 - Aar1 Cloning System
Parts submitted to the Registry
GOLD MEDAL Requisites
UCSF iGEM 2009 is sponsored by...
