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Week8
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1. Repeated FACS run of CFP and YFP with 100, 250, 500 and 1000 microM/ml IPTG | 1. Repeated FACS run of CFP and YFP with 100, 250, 500 and 1000 microM/ml IPTG | ||
| - | 2. Preparation of requirements for transformation. | + | 2. Preparation of requirements for transformation. (0.1M cacl2 soln. , Ice cold centrifuge tubes.) Kept autoclaved |
| + | |||
| + | eppendorf tubes and tips into freeze. | ||
3. Kanamycin stock 100X (X = 50 microg/ml) | 3. Kanamycin stock 100X (X = 50 microg/ml) | ||
Latest revision as of 01:42, 22 October 2009
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1. Repeated FACS run of CFP and YFP with 100, 250, 500 and 1000 microM/ml IPTG 2. Preparation of requirements for transformation. (0.1M cacl2 soln. , Ice cold centrifuge tubes.) Kept autoclaved eppendorf tubes and tips into freeze. 3. Kanamycin stock 100X (X = 50 microg/ml) |
