Team:Utah State/Notebook

From 2009.igem.org

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       <td width="172" id="ana"><span class="currentPage"<font size = 4>NOTEBOOK</font></span>
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         <a href="#Meeting">Meeting Notes </a><br />
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         <a href="#meeting">Meeting Notes </a><br />
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         <a href="#Notebook">Lab Notebook</a><br />
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         <a href="#notebook">Lab Notebook</a><br />
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         <a href="#Protocols">Protocols</a><br/>
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         <a href="#protocols">Protocols</a><br/>
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Notes from all of our Meetings
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Official Meetings  
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<b><i>May 12<b><i>
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Introduction to iGEM and to team members. Reviewed last years competition. Overview of the iGEM website.
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<p class="header">May 12</p>
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Plan-Become familiar with website, review team projects from previous years, create user accounts.
+
 
-
June 23
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<p class = "class">Introduction to team members and to iGEM. Reviewed last year’s competition and last year’s team contribution. Introduction also to the 2009 iGEM home page and our team wiki. </p>
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Discussed University of Hawaii team project from 2008. Watched their presentation and discussed their project goals. Decided as a group to continue their project where they left off, after having made contact with them.
+
 
-
Plan-Request constructs and parts from team Hawaii. Have conference call with last year's Hawaii team. Literature search.
+
<p class="header">June 23</p>
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June 30
+
 
-
Team introduction to working with a wiki. Waiting for constructs.
+
<p class = "class">After researching previous projects and brainstorming since our last meeting, we discussed the possibility of continuing the project that University of Hawaii initiated last year. We spoke with an advisor from their team and agreed that we could continue the project. Our team agreed that it would be a good project foundation.</p>
-
Plan-Contribute name, brief autobiographical description, and picture to wiki.
+
 
-
August ?
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<p class="header">June 30</p>
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Project Progress Check.
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Broad-Host Vector: After PCR, ligation attempts, gel observations, it appears that the Broad-Host Vector in the catalog is not functioning nor is it the anticipated length. Intend to take a known broad-host vector, perform an enzyme digestion and test ability of vector to carry red flourescent protein sequence.
+
<p class = "class">Small workshop on programming a wiki. Further collaboration with team Hawaii has taken place and we are waiting for DNA constructs with which they worked. </p>
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September 3
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September 10
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<p class="header">August 20</p>
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Finalized team roster and travel information. Reviewed judging criteria, our current standing as far as BioBricks designed, what could still be designed and feasibly tested. Discussed the track in which we would compete. Assignments made for next meeting.
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Plan-Complete abstract, finalize title and track by Sept. 18.
+
<p class = "class">Discussed progress made with broad-host vector construction.  The broad-host vector constructs that we have been working with from Team Hawaii and from the iGEM parts catalog do not appear to be functioning.  After PCR, attempted ligations, enzymatic digestions and electrophoretic gel observations, we’ve decided to move on and try to modify another known broad-host vector to be compatible with the BioBrick format. </p>
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1. Cole Peterson - Set up ligation of pRL1383a/BBa_I20260 and test.
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2. Alex Hatch - Submit schedule for availability to Cole, assist Cole with vector construction, write team background and post on the wiki.
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<p class="header">September 3</p>
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3. Garrett Hinton - Write a general project description for the front page of the wiki (see last years wiki, and other team's wiki's to get an idea of what you should write about and ask Libby, Trent, or Cole if you've any questions.
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4. Tyrell Rupp- Have a draft-version of a flash animation for our project.
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<p class = "class">Team discussion of PHAs, phasin, silver-fusion, and secretory pathways.  Further discussion of attempted broad-host vector modifications. </p>
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5. Rachel Porter- Have 3 logo ideas ready for the team to choose from.
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-
6. Jody Jerez/Jeff Karren- Edit/Post protocols on Wiki (you will need to contact other people in lab for help with revisions). Edit these so that they are also not just cut and paste from last years.
+
<p class="header">September 10</p>
-
September 17
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Had final discussions on project title and track we intend to compete in. Reviewed the project abstract. Discussed Wiki progress.
+
<p class = "class">Reviewed judging criteria and reviewed our standing with the broad-host vector and the secretion pathways. Discussed which tracks would be most applicable to our project. Discussed titles for our project. Finalization of team roster and travel information.   </p>
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</p>
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<p class="header">September 17</p><br>
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  </tr></tr>
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 +
<p class = "class">Made final decisions for our intended track, chose a final project title, and gave a final review of our abstract.  Discussed our wiki progress. </p>
 +
 +
<p class="header">September 24</p><br>
 +
 
 +
<p class = "class">Took team picture. Presentation of different team logo options and team shirt design options.  Flash animation presentation to be used potentially in wiki and presentation.
 +
 
 +
<p class="header">September 29</p><br>
 +
 
 +
<p class = "class">Team meeting with internet programming advisor. Discussed final formatting options for wiki. </p>
 +
 
 +
<p class="header">October 1</p><br>
 +
 
 +
<p class = "class">Instruction given on tri-parental mating.  Discussed selective plates and media for tri-parental mating. </p>
 +
 +
<p class="header">October 6</p><br>
 +
 
 +
<p class = "class">Instruction given on Western Blot procedure and function. Discussed modified GFP construct.  Final team logo was presented.</p>
 +
 
 +
<p class="header">October 8</p><br>
 +
 
 +
 
 +
<p class = "class">Met shortly and separated to work on different assignments for wiki. </p>
 +
 
 +
<p class="header">October 13</p><br>
 +
 
 +
<p class = "class">Final T-shirt design presented to team.  Reviewed completed parts and discussed broad-host vector. Presentation of completed secretion pathway models.  </p>
 +
 +
 
 +
<p class="header">October 15</p><br>
 +
 
 +
 
 +
<p class = "class">Discussed portions of the wiki that needed to be completed before the weekend. Reviewed project components and iGEM basics in preparation for the jamboree. </p>
 +
 
 +
<p class="header">October 20</p><br>
 +
 
 +
 
 +
<p class = "class">Final meeting before wiki closure.  Discussed last minute assignments to ensure that the wiki is completed.</p>
 +
 
 +
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<!---Lab Notebook--->
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Broad-Host Range Vectors
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Laboratory Notebook
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Vector text
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Members of our team each had individual lab notebooks. Rather than outline each procedure that was run by each individual person, we have instead decided to present our wiki lab notebook as a weekly update up the progress that was made. Many of the various procedure details and specifics are found below in the protocols section. </p>
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<div align="center"><img src="https://static.igem.org/mediawiki/2009/1/17/Notebooksusu.jpg"  align = "middle" height="200" style="padding:.5px; border-style:solid; border-color:#999" alt="Notebooks"> </div>
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<div align="center"><font size="2.5" face="Helvetica, Arial, San Serif" color =#231f20>
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<b>Figure 1.</b>  Some of our laboratory notebooks
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Secretion: GFP, Phasins, and Bioplastics
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Protocols
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This is where all of the text about secretion goes - First talk about secretion in general and why we care (downstream processing, etc.); next talk about what exactly we are looking at as an example (phasin/bioplastic secretion, along with GFP as an easier-to-monitor example);
+
Members of our team each had individual lab notebooks. Rather than outline each procedure that was run by each individual person, we have instead decided to present our wiki lab notebook as a weekly update up the progress that was made. Many of the various procedure details and specifics are found below in the protocols section. </p>
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Polyhydroxyalkanoates
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<br>
 +
<div align="center"><font size="2.5" face="Helvetica, Arial, San Serif" color =#231f20>
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<b>Figure 1.</b>  Some of our laboratory notebooks
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Revision as of 01:42, 22 October 2009

USU iGem Untitled Document

NOTEBOOK Meeting Notes
Lab Notebook
Protocols
Notebook

This page is for the notebook - this includes notes from our official team meetings, weekly progress updates, and the lab protocols.


Official Meetings

May 12

Introduction to team members and to iGEM. Reviewed last year’s competition and last year’s team contribution. Introduction also to the 2009 iGEM home page and our team wiki.

June 23

After researching previous projects and brainstorming since our last meeting, we discussed the possibility of continuing the project that University of Hawaii initiated last year. We spoke with an advisor from their team and agreed that we could continue the project. Our team agreed that it would be a good project foundation.

June 30

Small workshop on programming a wiki. Further collaboration with team Hawaii has taken place and we are waiting for DNA constructs with which they worked.

August 20

Discussed progress made with broad-host vector construction. The broad-host vector constructs that we have been working with from Team Hawaii and from the iGEM parts catalog do not appear to be functioning. After PCR, attempted ligations, enzymatic digestions and electrophoretic gel observations, we’ve decided to move on and try to modify another known broad-host vector to be compatible with the BioBrick format.

September 3

Team discussion of PHAs, phasin, silver-fusion, and secretory pathways. Further discussion of attempted broad-host vector modifications.

September 10

Reviewed judging criteria and reviewed our standing with the broad-host vector and the secretion pathways. Discussed which tracks would be most applicable to our project. Discussed titles for our project. Finalization of team roster and travel information.

September 17


Made final decisions for our intended track, chose a final project title, and gave a final review of our abstract. Discussed our wiki progress.

September 24


Took team picture. Presentation of different team logo options and team shirt design options. Flash animation presentation to be used potentially in wiki and presentation.

September 29


Team meeting with internet programming advisor. Discussed final formatting options for wiki.

October 1


Instruction given on tri-parental mating. Discussed selective plates and media for tri-parental mating.

October 6


Instruction given on Western Blot procedure and function. Discussed modified GFP construct. Final team logo was presented.

October 8


Met shortly and separated to work on different assignments for wiki.

October 13


Final T-shirt design presented to team. Reviewed completed parts and discussed broad-host vector. Presentation of completed secretion pathway models.

October 15


Discussed portions of the wiki that needed to be completed before the weekend. Reviewed project components and iGEM basics in preparation for the jamboree.

October 20


Final meeting before wiki closure. Discussed last minute assignments to ensure that the wiki is completed.

Go to Top of Page

Laboratory Notebook

Members of our team each had individual lab notebooks. Rather than outline each procedure that was run by each individual person, we have instead decided to present our wiki lab notebook as a weekly update up the progress that was made. Many of the various procedure details and specifics are found below in the protocols section.


Notebooks

Figure 1. Some of our laboratory notebooks
Go to Top of Page

Protocols

Members of our team each had individual lab notebooks. Rather than outline each procedure that was run by each individual person, we have instead decided to present our wiki lab notebook as a weekly update up the progress that was made. Many of the various procedure details and specifics are found below in the protocols section.


Notebooks

Figure 1. Some of our laboratory notebooks
Go to Top of Page


Retrieved from "http://2009.igem.org/Team:Utah_State/Notebook"