Team:UC Davis/PhoR ChvI1

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Latest revision as of 02:43, 22 October 2009

PhoR to ChvI

PhoR-->ChvI

It has been hypothesized that due to the amino acid similarity of ChvI to PhoB, ChvI is able to act as a phosphate regulator in E.coli as a PhoB substitute (3).

Since ChvI showed no abnormal activity inside Agrobacterium tumefaciens under phosphate limitation, it cannot serve as the primary phosphate regulator in A. tumefaciens (3).

Note: ChvI was not required for phosphate regulation in A. tumefaciens (3), but it might be involved in phosphate regulation in A. tumefaciens in some way (3). Therefore, the possibility of crosstalk still exists

Due to the structural similarity of ChvI and PhoB, the possibility of crosstalk exists (3).

Note: Since we are going to use E. coli as our model chassis for this project; we had to make sure that our proteins will keep their characteristics of our interest after being placed inside the E. coli. For example, VirG gene (promoter 1) after being introduced into E. coli cells, started to behave as if it were a member of phosphate regulon (note: PhoB function was necessary for virG induction by phosphate starvation)(6). Studies have suggested that the reason for this behavior was due to the fact that virG promoter 1 has two hexamer blocks which have the same positions as the pho genes (6).

Studies have shown that ChvI probably activated Alkaline Phosphatase (AP) activity by activating transcription of PhoA (PhoA also gets activated by PhoB) (3). It was not proven that ChvI activates other PhoB-dependent promoters or recognizes the same DNA sequence as PhoB (3). However, there has been speculation that since PhoR mediates PhoB phosphorylation and dephosphorylation, it may do the same to ChvI(3).

Therefore, the best option would be to find a promoter from A. tumefaciens that gets activated by ChvG/ChvI, a two-component regulatory system only; and does not interact with any E. coli system.

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-<div style="text-align: center;"><b style="color: rgb(255, 255, 153);"><span
+<div style="text-align: center;"><b style="color: rgb(0, 0, 0);">&nbsp;
+</b><b style="color: rgb(255, 255, 153);"><span
 style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
-href="https://2009.igem.org/Team:UC_Davis/homepage2"><img alt=""
+href="https://2009.igem.org/Team:UC_Davis"><img alt=""
 src="https://static.igem.org/mediawiki/2009/a/a4/UCDAVIS_PIC3.png"
 style="border: 0px solid ; width: 83px; height: 36px;"></a> </big></big></big></big></span></b><a
-href="https://2009.igem.org/Team:UC_Davis/About_Us1"><b
+href="https://2009.igem.org/Team:UC_Davis/About_Us"><b
 style="color: rgb(255, 255, 153);"><span
 style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><img
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 style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big>
 </big></big></big></big></span></b><b style="color: rgb(255, 255, 153);"><span
+style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"></span></b><b
+style="color: rgb(255, 255, 153);"><span
 style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
-href="https://2009.igem.org/Team:UC_Davis/Project1"><img alt=""
+href="https://2009.igem.org/Team:UC_Davis/Project"><img alt=""
 src="https://static.igem.org/mediawiki/2009/b/b9/UCDAVIS_PIC8.png"
 style="border: 0px solid ; width: 78px; height: 36px;"></a> </big></big></big></big></span></b><b
 style="color: rgb(255, 255, 153);"><span
 style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
-href="https://2009.igem.org/Team:UC_Davis/Notebook1"><img alt=""
+href="https://2009.igem.org/Team:UC_Davis/Notebook"><img alt=""
 src="https://static.igem.org/mediawiki/2009/2/2f/UCDAVIS_PIC5.png"
 style="border: 0px solid ; width: 81px; height: 36px;"></a> </big></big></big></big></span></b><b
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 style="color: rgb(255, 255, 153);"><span
 style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big><big><big><big><a
-href="https://2009.igem.org/Team:UC_Davis/Part1"><img alt=""
+href="https://2009.igem.org/Team:UC_Davis/Parts"><img alt=""
 src="https://static.igem.org/mediawiki/2009/a/a6/UCDAVIS_PIC6.png"
 style="border: 0px solid ; width: 78px; height: 37px;"></a>&nbsp;<a
-href="https://2009.igem.org/Team:UC_Davis/Contact1"><img alt=""
+href="https://2009.igem.org/Team:UC_Davis/Contacts_References"><img
-src="https://static.igem.org/mediawiki/2009/1/1d/UCDAVIS_PIC7.png"
+alt="" src="https://static.igem.org/mediawiki/2009/1/1d/UCDAVIS_PIC7.png"
 style="border: 0px solid ; width: 83px; height: 37px;"></a></big></big></big></big></span></b></div>
 <hr
@@ Line 48: / Line 51: @@
 <br>
 </span></big></big>&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
-&nbsp;&nbsp;&nbsp; It has been hypothesized
+&nbsp;&nbsp;&nbsp; </span></b><span
+style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">It
+has been hypothesized
 that due to the amino acid similarity of ChvI to PhoB, ChvI is able to
 act as a
-phosphate regulatory in <i>E.coli</i>, as a PhoB substitute (3).</span></b><span
+phosphate regulator in <i>E.coli</i> as a PhoB substitute (3).</span><span
 style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"><o:p></o:p></span></p>
 <p class="MsoNormal"
-style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><b><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><span
 style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">&nbsp;&nbsp;&nbsp;
 <span style="">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>Since
 ChvI showed no abnormal activity
-inside <i>Agrobacterium</i> <i>Tumefaciens</i> under phosphate
+inside <i>Agrobacterium tumefaciens</i> under phosphate
 limitation, it
-cannot serve as the primary phosphate regulator in <i>Agrobacterium</i>
+cannot serve as the primary phosphate regulator in <i>A. tumefaciens</i>
-(3).<o:p></o:p></span></b></p>
+(3).<o:p></o:p></span></p>
 <p class="MsoNormal"
-style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><b><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><span
 style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"><span
 style="">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 </span><i>Note: ChvI was not required for phosphate regulation
-in Agrobacterium Tumefaciens (3), but it might be involved in phosphate
+in A. tumefaciens (3), but it might be involved in phosphate
-regulation in Agrobacterium Tumefaciens in some way (3). Therefore, the
+regulation in A. tumefaciens in some way (3). Therefore, the
-possibility of crosstalk still exists</i><o:p></o:p></span></b></p>
+possibility of crosstalk still exists</i><o:p></o:p></span></p>
 <p class="MsoNormal"
-style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><b><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><span
 style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">&nbsp;&nbsp;<span
 style="">&nbsp;&nbsp; </span>&nbsp;Due to the structural similarity
 of
-ChvI and PhoB, the possibility of crosstalk exists (3).<o:p></o:p></span></b></p>
+ChvI and PhoB, the possibility of crosstalk exists (3).<o:p></o:p></span></p>
-<p class="MsoNormal"><b><i><span
+<p class="MsoNormal"><i><span
 style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">&nbsp;&nbsp;&nbsp;
 Note: Since
-we are going to use Escherichia coli as our model chassis for this
+we are going to use E. coli as our model chassis for this
 project; we
 had to make sure that our proteins will keep their characteristics of
 our
-interest after being placed inside the Escherichia coli.</span></i></b><b><span
+interest after being placed inside the E. coli.</span></i><span
 style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">
 <i>For example, VirG gene (promoter 1) after being introduced
-into E.coli cells, started to behave as if it were a member of
+into E. coli cells, started to behave as if it were a member of
 phosphate
 regulon (note: PhoB function was necessary for virG induction by
@@ Line 94: / Line 99: @@
 was due to the fact that virG promoter 1 has two hexamer blocks which
 have the
-same positions as the pho genes (6).</i> <o:p></o:p></span></b></p>
+same positions as the pho genes (6).</i> <o:p></o:p></span></p>
 <p class="MsoNormal"
-style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><b><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><span
 style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"><span
 style="">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 </span>Studies have shown that ChvI probably activated Alkaline
-Phosphatase</span></b><em><span
+Phosphatase</span><em><span
-style="font-size: 13pt; font-family: &quot;Calibri&quot;,&quot;sans-serif&quot;;"> </span></em><em><b><span
+style="font-size: 13pt; font-family: &quot;Calibri&quot;,&quot;sans-serif&quot;;"> </span></em><em><span
-style="font-size: 13pt;">(AP)</span></b></em><b><span
+style="font-size: 13pt;">(AP)</span></em><span
 style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">
 activity by activating transcription of PhoA (PhoA also gets activated
@@ Line 108: / Line 113: @@
 (3). It was not proven that ChvI activates other PhoB-dependent
 promoters or
-recognizes the same DNA sequence as PhoB (3).But, there has been
+recognizes the same DNA sequence as PhoB (3). However, there has been
 speculation
 that since PhoR mediates PhoB phosphorylation and dephosphorylation, it
 may do
-the same to ChvI(3).<o:p></o:p></span></b></p>
+the same to ChvI(3).<o:p></o:p></span></p>
 <p class="MsoNormal"
-style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><b><span
+style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><span
-style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"><span
+style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-style="">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+Therefore, the best option would be to find a promoter
-</span>Therefore, the best option would be to find a promoter
+from <i>A. tumefaciens</i> that gets activated by ChvG/ChvI, a
-from <i>Agrobacterium</i> that gets activated by ChvG/ChvI, a
 two-component
 regulatory system only; and does not interact with any <i>E. coli</i>
-system.<br>
+system.</span><b><span
+style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"><br>
 </span></b></p>
 <hr style="width: 100%; height: 2px;">