Team:IBB Pune/project/Results

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{{Team:IBB_Pune/header}}
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{{Team:IBB_Pune/menu}}
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<br>
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<p><span style="font-weight:bold; font-size:200%; color:#0000cc;">Characterization of And Gates:</span></p><br>
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Even as the wiki freezes we are characterizing Const.Prom-LacO-mRFP for its expression. We hope we get positive results.
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<br>
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<p><span style="font-weight:bold; font-size:200%; color:#0000cc;">We carried out the following transformations successfully:</span></p><br>
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<p><span style="font-weight:bold; font-size:150%; color:#FF6600;">Native Biobricks</span></p>
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# pLL promoter              - BBa_I746365
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# GFP coding sequence        - BBa_E0040
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# RFP coding sequence
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# GFP Translational unit
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# RFP Translational unit
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# Enhanced CFP
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# Fast GFP
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# Constitutive promoter      -BBa_J23102
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# Promoter + RBS
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# T7 promoter
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# pLuxR
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# Double terminator
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# RBS
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# Constitutive LuxI
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# LuxR Translational unit
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<p><span style="font-weight:bold; font-size:150%; color:#FF6600;">OUR PARTS</span></p>
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# YcdB
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# TorA
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# Lac Operator
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<p><span style="font-weight:bold; font-size:150%; color:#FF6600;">Assembly</span></p>
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# Const. Promoter-LacO
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# PluxR- LuxI
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# {Promoter + RBS}-GFP
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# {Promoter + RBS}-TorA
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# {Promoter + RBS}-Ycdb
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# luxR-PluxR-{RBS-LuxI}
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# Promoter + RBS
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# YcdB-GFP
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# TorA-GFP
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# {Promoter + RBS}-LuxR
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# {Promoter + RBS}-YcdB-GFP
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# {Promoter + RBS}-TorA-GFP
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# Const. Promoter-LuxR
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<p><span style="font-weight:bold; font-size:175%; color:#FF6600;">Failed Transformations</span></p>
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<p><span style="font-weight:bold; font-size:150; color:#FF6600;">Backbones</span></p>
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# pSB1AK3 with CcdB gene
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# pSB1T3 with CcdB gene
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# pSB1C3 with CcdB gene
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# pSB1K3 with CcdB gene
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# pSB1A3 with CcdB gene
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# pSB1AC3 with CcdB gene
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# pSB1AK3 with mRFP
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# pSB1T3 with mRFP
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# pSB1C3 with mRFP
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# pSB1K3 with mRFP
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# pSB1A3 with mRFP
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# pSB1AC3 with mRFP
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<p><span style="font-weight:bold; font-size:150; color:#FF6600;">Native Biobricks</span></p>
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# Lysis Cassette
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# Constitutive GFP
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# COnstitutive CFP
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<p><span style="font-weight:bold; font-size:150; color:#FF6600;">GEL PICTURES</span></p>
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[[Image:10sepgel.jpg|center|500px]]
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<br><br>
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[[Image:14octgel.jpg|center|500px]]
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<html>
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<table border="0" align="center">
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<tr><td>Lane1</td><td>Lane2</td><td>Lane3</td> <td>Lane4</td><td>Lane5</td><td>Lane6</td><td>Lane7</td></tr>
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<tr><td>TorA</td><td>YcdB</td><td>YcdB</td><td>LacO</td><td>LacO</td><td>pSB1AK3</td><td>pSB1AK3</td></tr></table>

Latest revision as of 02:44, 22 October 2009





Characterization of And Gates:


Even as the wiki freezes we are characterizing Const.Prom-LacO-mRFP for its expression. We hope we get positive results.


We carried out the following transformations successfully:


Native Biobricks


  1. pLL promoter - BBa_I746365
  2. GFP coding sequence - BBa_E0040
  3. RFP coding sequence
  4. GFP Translational unit
  5. RFP Translational unit
  6. Enhanced CFP
  7. Fast GFP
  8. Constitutive promoter -BBa_J23102
  9. Promoter + RBS
  10. T7 promoter
  11. pLuxR
  12. Double terminator
  13. RBS
  14. Constitutive LuxI
  15. LuxR Translational unit

OUR PARTS


  1. YcdB
  2. TorA
  3. Lac Operator


Assembly


  1. Const. Promoter-LacO
  2. PluxR- LuxI
  3. {Promoter + RBS}-GFP
  4. {Promoter + RBS}-TorA
  5. {Promoter + RBS}-Ycdb
  6. luxR-PluxR-{RBS-LuxI}
  7. Promoter + RBS
  8. YcdB-GFP
  9. TorA-GFP
  10. {Promoter + RBS}-LuxR
  11. {Promoter + RBS}-YcdB-GFP
  12. {Promoter + RBS}-TorA-GFP
  13. Const. Promoter-LuxR



Failed Transformations


Backbones

  1. pSB1AK3 with CcdB gene
  2. pSB1T3 with CcdB gene
  3. pSB1C3 with CcdB gene
  4. pSB1K3 with CcdB gene
  5. pSB1A3 with CcdB gene
  6. pSB1AC3 with CcdB gene
  7. pSB1AK3 with mRFP
  8. pSB1T3 with mRFP
  9. pSB1C3 with mRFP
  10. pSB1K3 with mRFP
  11. pSB1A3 with mRFP
  12. pSB1AC3 with mRFP

Native Biobricks


  1. Lysis Cassette
  2. Constitutive GFP
  3. COnstitutive CFP

GEL PICTURES

10sepgel.jpg



14octgel.jpg

Lane1Lane2Lane3 Lane4Lane5Lane6Lane7
TorAYcdBYcdBLacOLacOpSB1AK3pSB1AK3

Retrieved from "http://2009.igem.org/Team:IBB_Pune/project/Results"