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Team:IPN-UNAM-Mexico/Parts
From 2009.igem.org
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| - | + | Regulatory LacI module coupled to the activator module with GFP reporter module. Basal conditions. 6,4x | |
| - | [[Image: | + | [[Image:1601.jpg | 350px|center]] |
| - | [[Image:1602.jpg | 350px| | + | Regulatory LacI module coupled to the activator module with GFP reporter module. IPTG 160mM. 6,4x |
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| + | [[Image:Control2.jpg | 350px|center]] | ||
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| + | Regulatory LacI module coupled to the activator module with GFP reporter module. Basal conditions. 19x | ||
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| + | [[Image:1602.jpg | 350px|center]] | ||
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| + | Regulatory LacI module coupled to the activator module with GFP reporter module. IPTG 160mM. 19x | ||
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Revision as of 02:45, 22 October 2009
Biobricks
Biobricks
We contribute to the registry with 11 new biobricks, 5 of them are favorite biobricks from the team and in this section we document them:
Main biobricks: modules and Projects
Modules
| Module and Status | Biobrick Name | Type | Image | Description |
|---|---|---|---|---|
| Mod 1 Sent | [http://partsregistry.org/Part:BBa_K266006 BBa_K266006] | Las AHL |  | BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the policistronic expresion of LasI enzyme and GFP, double terminator. |
| Mod 2 Sent | [http://partsregistry.org/Part:BBa_K266004 BBa_K266004] | Lac inverter |  | Constitutive promoter J23100 with Lac system inverter controlling the expression of LasR. |
| Mod 3 Sent | [http://partsregistry.org/Part:BBa_K266001 BBa_K266001] | Tet inverter |  | J23100 constitutive promoter directs the expression of tetracycline repressor (TetR). TetR binds to pTet regulatory region resulting in a negative control of the production of LuxR. The whole system acts as an Inverter of Tet system controlling LuxR expression. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. |
| Mod 4 Sent | [http://partsregistry.org/Part:BBa_K266000 BBa_K266000] | Lux AHL |  | This biobrick has a promoter inducible by PAI+LasR (BBa_R0079) I.e. positive regulation and produces LuxI enzyme (BBa_F1610). This enzyme produces 3OC6HSL (AI). |
Projects
These two biobricks are the main Activator-Inhibitor system, as showned below they are in two different plasmids, and the idea is transform Top10 cells with them in petri dishes with IPTG and ATC.
| Status | Biobrick Name | Image | Description |
|---|---|---|---|
| Sent | [http://partsregistry.org/Part:BBa_K266007 BBa_K266007] |  | Complex Quorum sensing circuit that receives the signal of PAI+LasR and AI+LuxR to control the production of LuxI and LasI enzymes. |
| Pending | [http://partsregistry.org/Part:BBa_K266010 BBa_K2660010] |  | Tet constitutive inverter controlling LasR expression and Lac constitutive inverter controlling LuxR expression. |
Auxiliary biobricks
This biobricks are though like construction intermediates necessary to build the main and project biobricks.
| Status | Biobrick Name | Image | Description |
|---|---|---|---|
| Sent | [http://partsregistry.org/Part:BBa_K266002 BBa_K266002] |  | LasR coding region and a double terminator. |
| Sent | [http://partsregistry.org/Part:BBa_K266005 BBa_K266005] |  | BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the expresion of LasI enzyme, no terminator. |
| Sent | [http://partsregistry.org/Part:BBa_K266008 BBa_K266008] |  | The BBa_J23100 constitutive promoter with Lac systems inverter. |
| Sent | [http://partsregistry.org/Part:BBa_K266009 BBa_K266009] |  | The BBa_J23100 constitutive promoter with Lac systems inverter and strong RBS. |
| Sent | [http://partsregistry.org/Part:BBa_K266011 BBa_K2660011] |  | POPS regulated and Lac inverter system with strong RBS. |
Plasmids
Results
Regulatory LacI module coupled to the activator module with GFP reporter module. Basal conditions. 6,4x
Regulatory LacI module coupled to the activator module with GFP reporter module. IPTG 160mM. 6,4x
Regulatory LacI module coupled to the activator module with GFP reporter module. Basal conditions. 19x
Regulatory LacI module coupled to the activator module with GFP reporter module. IPTG 160mM. 19x
