Team:TorontoMaRSDiscovery/Notebook

From 2009.igem.org

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{| style="color:white;background-color:#99CCFF;" height:100px cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu"
{| style="color:white;background-color:#99CCFF;" height:100px cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu"
!align="center"|[[Team:TorontoMaRSDiscovery|Home]]
!align="center"|[[Team:TorontoMaRSDiscovery|Home]]
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!align="center"|[[Team:TorontoMaRSDiscovery/Project|The Project]]
!align="center"|[[Team:TorontoMaRSDiscovery/Project|The Project]]
!align="center"|[[Team:TorontoMaRSDiscovery/Parts|BioBricks]]
!align="center"|[[Team:TorontoMaRSDiscovery/Parts|BioBricks]]
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!align="center"|[[Team:TorontoMaRSDiscovery/Modeling|Modeling]]
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!align="center"|[[Team:TorontoMaRSDiscovery/Modeling|Modelling]]
!align="center"|[[Team:TorontoMaRSDiscovery/Bioinformatics|Bioinformatics]]
!align="center"|[[Team:TorontoMaRSDiscovery/Bioinformatics|Bioinformatics]]
!align="center"|[[Team:TorontoMaRSDiscovery/Safety|Safety]]
!align="center"|[[Team:TorontoMaRSDiscovery/Safety|Safety]]
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==Summary of events==
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==Summary of Results==
{| border="1" cellpadding="5" cellspacing="0"
{| border="1" cellpadding="5" cellspacing="0"
-
|-
 
|'''Part Type'''
|'''Part Type'''
|'''Arbitrary Name'''
|'''Arbitrary Name'''
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|'''Status'''
|'''Status'''
|'''Transformants Stocked?'''
|'''Transformants Stocked?'''
-
|'''Antibiotic Resistance, Backbone Plasmid Visualized?'''
+
|'''Antibiotic Resistant, Backbone Plasmid Visualized?'''
|'''Part Visualized?'''
|'''Part Visualized?'''
|'''Sequenced?'''
|'''Sequenced?'''
-
|-
+
|----
|rowspan="2"|
|rowspan="2"|
'''New Parts'''
'''New Parts'''
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|Yes
|Yes
|Yes
|Yes
-
|-
+
|----
|eCFPtgt
|eCFPtgt
-
|
+
|K192001
-
|CFP Target Protein
+
|CFP target protein
|Synthesized by Mr Gene
|Synthesized by Mr Gene
|No
|No
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|No
|No
|Yes
|Yes
-
|-
+
|----
|rowspan="13"|
|rowspan="13"|
'''Registry Parts'''
'''Registry Parts'''
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|Yes
|Yes
|Yes
|Yes
-
|Too Small
+
|Too small
|No
|No
-
|-
+
|----
|2
|2
|B0034
|B0034
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|Yes
|Yes
|Yes
|Yes
-
|Too Small
+
|Too small
|No
|No
-
|-
+
|----
|3
|3
|C0040
|C0040
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|Yes
|Yes
|No
|No
-
|-
+
|----
|4
|4
|C0012
|C0012
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|Yes
|Yes
|No
|No
-
|-
+
|----
|5
|5
|B0015
|B0015
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|Yes
|Yes
|Yes
|Yes
 +
|No
 +
|----
 +
|C (Amp + C)
 +
|pSB1AC3
 +
|Assembly
 +
|Confirmed
 +
|Yes
 +
|Yes
 +
|Yes
 +
|No
 +
|----
 +
|C (C only)
 +
|pSB1C3
 +
|Assembly
 +
|Confirmed
 +
|Yes
 +
|Yes
 +
|Yes
 +
|No
 +
|----
 +
|K (ccdb)
 +
|pSB1AK3
 +
|Assembly
 +
|Confirmed
 +
|Yes
 +
|Yes
 +
|Yes
 +
|No
 +
|----
 +
|K (RFP)
 +
|pSB1K3
 +
|Assembly
 +
|Confirmed
 +
|Yes
 +
|Yes
 +
|Yes
 +
|No
 +
|----
 +
|Tet (ccdb)
 +
|pSB1AT3
 +
|Assembly
 +
|Confirmed
 +
|Yes
 +
|Yes
 +
|Yes
 +
|No
 +
|----
 +
|Tet (RFP)
 +
|pSB1T3
 +
|Assembly
 +
|Confirmed
 +
|Yes
 +
|Yes
 +
|Yes
 +
|No
 +
|----
 +
|7
 +
|J13002
 +
|Encapsulin
 +
|Transfected
 +
|Yes
 +
|No
 +
|No
 +
|No
 +
|----
 +
|Amp
 +
|pSB1A3
 +
|Assembly
 +
|Transfected
 +
|Yes
 +
|No
 +
|No
|No
|No
|-
|-
 +
|rowspan="7"|
 +
'''Assembled Parts'''
 +
|----
 +
|Enc in C (Amp+C)
 +
|
 +
|Submission, Encapsulin
 +
|Confirmed
 +
|Yes
 +
|Yes
 +
|Yes
 +
|Yes
 +
|----
 +
|1+2 in C (Amp+C)
 +
|
 +
|Control, Encapsulin
 +
|Confirmed
 +
|Yes
 +
|Yes
 +
|Too small
 +
|No
 +
|----
 +
|3+2 in C (Amp+C)
 +
|
 +
|Control
 +
|Confirmed
 +
|Yes
 +
|Yes
 +
|Yes
 +
|No
 +
|----
 +
|4+5 in Tet (ccdb)
 +
|
 +
|Control
 +
|Confirmed
 +
|Yes
 +
|Yes
 +
|Yes
 +
|No
 +
|----
 +
|1+2+3+2 in K (RFP)
 +
|
 +
|Control
 +
|Transfected
 +
|Yes
 +
|No
 +
|No
 +
|No
 +
|----
 +
|1+2+Enc in K
 +
|
 +
|Encapsulin
 +
|Confirmed
 +
|Yes
 +
|Yes
 +
|Yes
 +
|In Progress
 +
|----
|}
|}

Latest revision as of 03:05, 22 October 2009

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Home The Team The Project BioBricks Modelling Bioinformatics Safety Notebook


Monthly Notebook

Summary of Results

Part Type Arbitrary Name Registry Code Construct Used For Status Transformants Stocked? Antibiotic Resistant, Backbone Plasmid Visualized? Part Visualized? Sequenced?

New Parts

Encapsulin (Enc) K192000 Encapsulin Confirmed Yes Yes Yes Yes
eCFPtgt K192001 CFP target protein Synthesized by Mr Gene No No No Yes

Registry Parts

1 JJ23100 Control, Encapsulin Confirmed Yes Yes Too small No
2 B0034 Control, Encapsulin Confirmed Yes Yes Too small No
3 C0040 Control Confirmed Yes Yes Yes No
4 C0012 Control Confirmed Yes Yes Yes No
5 B0015 Control, Encapsulin Confirmed Yes Yes Yes No
C (Amp + C) pSB1AC3 Assembly Confirmed Yes Yes Yes No
C (C only) pSB1C3 Assembly Confirmed Yes Yes Yes No
K (ccdb) pSB1AK3 Assembly Confirmed Yes Yes Yes No
K (RFP) pSB1K3 Assembly Confirmed Yes Yes Yes No
Tet (ccdb) pSB1AT3 Assembly Confirmed Yes Yes Yes No
Tet (RFP) pSB1T3 Assembly Confirmed Yes Yes Yes No
7 J13002 Encapsulin Transfected Yes No No No
Amp pSB1A3 Assembly Transfected Yes No No No

Assembled Parts

Enc in C (Amp+C) Submission, Encapsulin Confirmed Yes Yes Yes Yes
1+2 in C (Amp+C) Control, Encapsulin Confirmed Yes Yes Too small No
3+2 in C (Amp+C) Control Confirmed Yes Yes Yes No
4+5 in Tet (ccdb) Control Confirmed Yes Yes Yes No
1+2+3+2 in K (RFP) Control Transfected Yes No No No
1+2+Enc in K Encapsulin Confirmed Yes Yes Yes In Progress
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