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<Font Size="7">'''''E''.coli Time Manager '''</Font> <Font Size="5">-Since 2008-</Font>  <html><a href="http://www.chiba-u.ac.jp/e/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/5/5f/Logo_chiba-u.gif" align="right" width="250"></a></html>
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{| style="color:white;" cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu" |align="center"
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!align="center"|[[Team:Chiba|Home]]
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!align="center"|[[Team:Chiba/Team|The Team]]
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!align="center"|[[Team:Chiba/Parts|Parts]]
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!align="center"|[[Team:Chiba/Reference|Reference]]
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
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!align="center"|[[Team:Chiba/Notebook/protocol|Protocols]]
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!align="center"|[[Team:Chiba/Links|Links]]
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
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!align="center"|[[Team:Chiba/Contact|Contact]]
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'''[[Team:Chiba/Project|The Project]]'''
'''[[Team:Chiba/Project|The Project]]'''
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*[[Team:Chiba/Project#Introduction|Introduction]]
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'''1,''' [[Team:Chiba/Project#Introduction|Introduction]]
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*[[Team:Chiba/Project#Project_Design|Project Design]]
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'''2,''' [[Team:Chiba/Project#Project_Design|Project Design]]
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*[[Team:Chiba/Project#Conclusions|Conclusions]]
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'''3,''' [[Team:Chiba/Project#Experiments,_Results_&_Discussion|Experiments, Results & Discussion]]
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'''[[Team:Chiba/Project/Delay_Switch|Delay Switch]]'''
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*[[Team:Chiba/Project/Delay_Switch#Experiments_(2008)|Experiments 2008]]
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3-1, [[Team:Chiba/Project#Making_LuxR_Mutants|Making LuxR Mutants]]
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*[[Team:Chiba/Project/Delay_Switch#Results_(2008)|Results 2008]]
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3-2, [[Team:Chiba/Project#Characterization_of_LuxR_Mutants|Characterization of LuxR Mutants]]
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*[[Team:Chiba/Project/Delay_Switch#Experiments_(2009)|Experiments 2009]]
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3-3, [[Team:Chiba/Project#Demonstration|Demonstration]]
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*[[Team:Chiba/Project/Delay_Switch#Results_(2009)|Results 2009]]
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'''5,''' [[Team:Chiba/Project#Conclusions|Conclusions]]
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'''[[Team:Chiba/Project/Parallel|Parallel Activation]]'''
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*[[Team:Chiba/Modeling|Modeling]]
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*[[Team:Chiba/Project/Parallel#Experiments|Experiments]]
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*[[Team:Chiba/Project/Parallel#Results|Results]]
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'''[[Team:Chiba/Project/Series|Series Activation]]'''
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*[[Team:Chiba/Modeling|Modeling]]
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*[[Team:Chiba/Project/Series#Experiments|Experiments]]
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<Font Size="6">''E''.coli Time Manager Since 2008</Font>  <html><a href="http://www.chiba-u.ac.jp/e/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/5/5f/Logo_chiba-u.gif" align="right" width="250"></a></html>
 
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{| style="color:white;" cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu" |align="center"
 
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!align="center"|[[Team:Chiba|Home]]
 
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!align="center"|[[Team:Chiba/Team|The Team]]
 
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!align="center"|[[Team:Chiba/Parts|Parts]]
 
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!align="center"|[[Team:Chiba/Reference|Reference]]
 
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
 
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!align="center"|[[Team:Chiba/Notebook/protocol|Protocols]]
 
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!align="center"|[[Team:Chiba/Links|Links]]
 
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
 
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!align="center"|[[Team:Chiba/Contact|Contact]]
 
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__NOTOC__
__NOTOC__
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==Bacterial Communication(Quorum Sensing)==
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== Reference ==
#[http://www.pnas.org/content/100/suppl.2/14549.full Michiko E. Taga. Bonnie　L.Bassler.:Chemical communication among bacteria.PNAS.November 25, 2003,'''100'''.suppl.2]
#[http://www.pnas.org/content/100/suppl.2/14549.full Michiko E. Taga. Bonnie　L.Bassler.:Chemical communication among bacteria.PNAS.November 25, 2003,'''100'''.suppl.2]
#[http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.micro.55.1.165?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dncbi.nlm.nih.gov Melissa B. Miller and Bonnie L. Bassler.:QUORUM SENSING IN BACTERIA.annurev.micro.55.1.165.2001]
#[http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.micro.55.1.165?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dncbi.nlm.nih.gov Melissa B. Miller and Bonnie L. Bassler.:QUORUM SENSING IN BACTERIA.annurev.micro.55.1.165.2001]
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#[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=38458 A. L. SCHAEFER ''et al.:''Generation of cell-to-cell signals in quorum sensing:Acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein.PNAS.93, pp. 9507, September 1996,Biochemistry]
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== Crosstalk ==
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#[http://authors.library.caltech.edu/5553/ C. H. Collins.''et al.:''Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones.Mol.Microbiol.2005.'''55'''(3).712–723]
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#[http://pubs.acs.org/cgi-bin/abstract.cgi/bichaw/2005/44/i08/abs/bi048005m.html Raychaudhuri.A ''et al''.:Chemical Mechanism and Substrate Specificity of RhlI, an Acylhomoserine Lactone Synthase from Pseudomonas aeruginosa.Biochemistry,44(8),2974 -2981,2005]
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#[http://www.pnas.org/content/91/1/197.abstract J P Pearson.''et al.:''Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes.PNAS,4,1994,'''91''',No. 1 ,197-201 ]
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#[http://www.pnas.org/content/92/20/9427.abstract M K Winson ''et al.:''Multiple N-acyl-L-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa.PNAS,(1995),'''92'''No.20,9427-9431]
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#[http://www3.interscience.wiley.com/journal/119124142/abstract M.K Winson ''et al.:''Construction and analysis of luxCDABE-based plasmid sensors　for investigating N-acyl homoserine lactone-mediated quorum sensing.FEMS Microbiology Letters 163 (1998) 185-192]
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#[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4PRHJ6K-2&_user=136872&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=136872&md5=3ed7ffab9f831d102f5e99353843c080 D. J. Sayut ''et al.:''Noise and kinetics of LuxR positive feedback loops.Biochem. Biophys. Res. Commun.'''363'''(3),2007,667-673.]
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#[http://www.springerlink.com/content/02704v4772858846/ W. nasser ''et al.:''New insights into the regulatory mechanisms of the LuxR family of quorum sensing regulators. Anal. Bioanal. Chem. '''387''',2007,381-390.]
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#[http://www.nature.com/nature/journal/v417/n6892/full/nature00833.html R.G.Zhang ''et al.:''Structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA. nature '''417''',2002,971-974.]

Latest revision as of 03:10, 22 October 2009

E.coli Time Manager


Home The Team Parts Reference Notebook Protocols Links Acknowledgements Contact

The Project

1, Introduction

2, Project Design

3, Experiments, Results & Discussion

3-1, Making LuxR Mutants

3-2, Characterization of LuxR Mutants

3-3, Demonstration

5, Conclusions

Reference

  1. [http://www.pnas.org/content/100/suppl.2/14549.full Michiko E. Taga. Bonnie　L.Bassler.:Chemical communication among bacteria.PNAS.November 25, 2003,100.suppl.2]
  2. [http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.micro.55.1.165?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dncbi.nlm.nih.gov Melissa B. Miller and Bonnie L. Bassler.:QUORUM SENSING IN BACTERIA.annurev.micro.55.1.165.2001]
  3. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=38458 A. L. SCHAEFER et al.:Generation of cell-to-cell signals in quorum sensing:Acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein.PNAS.93, pp. 9507, September 1996,Biochemistry]
  4. [http://authors.library.caltech.edu/5553/ C. H. Collins.et al.:Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones.Mol.Microbiol.2005.55(3).712–723]
  5. [http://pubs.acs.org/cgi-bin/abstract.cgi/bichaw/2005/44/i08/abs/bi048005m.html Raychaudhuri.A et al.:Chemical Mechanism and Substrate Specificity of RhlI, an Acylhomoserine Lactone Synthase from Pseudomonas aeruginosa.Biochemistry,44(8),2974 -2981,2005]
  6. [http://www.pnas.org/content/91/1/197.abstract J P Pearson.et al.:Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes.PNAS,4,1994,91,No. 1 ,197-201 ]
  7. [http://www.pnas.org/content/92/20/9427.abstract M K Winson et al.:Multiple N-acyl-L-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa.PNAS,(1995),92No.20,9427-9431]
  8. [http://www3.interscience.wiley.com/journal/119124142/abstract M.K Winson et al.:Construction and analysis of luxCDABE-based plasmid sensors　for investigating N-acyl homoserine lactone-mediated quorum sensing.FEMS Microbiology Letters 163 (1998) 185-192]
  9. [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4PRHJ6K-2&_user=136872&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=136872&md5=3ed7ffab9f831d102f5e99353843c080 D. J. Sayut et al.:Noise and kinetics of LuxR positive feedback loops.Biochem. Biophys. Res. Commun.363(3),2007,667-673.]
  10. [http://www.springerlink.com/content/02704v4772858846/ W. nasser et al.:New insights into the regulatory mechanisms of the LuxR family of quorum sensing regulators. Anal. Bioanal. Chem. 387,2007,381-390.]
  11. [http://www.nature.com/nature/journal/v417/n6892/full/nature00833.html R.G.Zhang et al.:Structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA. nature 417,2002,971-974.]
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