Team:LCG-UNAM-Mexico/AbrahamJurnal
From 2009.igem.org
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I started working in the new lab. | I started working in the new lab. | ||
- | Objectives: | + | |
+ | '''== Objectives: ==''' | ||
+ | |||
The final aim is to earn all the following devices and them all together into the plasmid we are suggesting, that will be extracted from P4 | The final aim is to earn all the following devices and them all together into the plasmid we are suggesting, that will be extracted from P4 | ||
[[Image:Sistema.JPG]] | [[Image:Sistema.JPG]] | ||
- | I learned to obtain the plasmid | + | I learned to obtain the plasmid sent by iGEM in the 2009 kit plates, and to transform it, the biobricks we are trying to obtain are: |
BBa_R1062 Promoter activated by LuxR-HSL complex <Br> BBa_I1352 RFP constitutively expressed and repressed with tetracycline<br> BBa_J37033 LuxR protein<br> BBa_C0261 AHL making enzyme<br> BBa_B0015 Double transcriptional terminator<br> BBa_P1003 Kanamycine ressistance casette<br> BBa_J04450 mRFP<br> | BBa_R1062 Promoter activated by LuxR-HSL complex <Br> BBa_I1352 RFP constitutively expressed and repressed with tetracycline<br> BBa_J37033 LuxR protein<br> BBa_C0261 AHL making enzyme<br> BBa_B0015 Double transcriptional terminator<br> BBa_P1003 Kanamycine ressistance casette<br> BBa_J04450 mRFP<br> | ||
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Final goals: | Final goals: | ||
#Ensamble and prove the functionality of the kamikaze device. | #Ensamble and prove the functionality of the kamikaze device. | ||
- | #Get the time in wich a colicin, | + | #Get the time in wich a colicin, prefferentially E3, kills E. coli c1-alpha. |
#Clone the bioparts received from Gene Art into any iGEM plasmid vector. Send them to the Registry of standard biological parts. | #Clone the bioparts received from Gene Art into any iGEM plasmid vector. Send them to the Registry of standard biological parts. | ||
Partial goals: | Partial goals: | ||
- | #Get the time in wich a colicin, | + | #Get the time in wich a colicin, prefferentially E3, kills Escherichia coli c1-alpha, the election of this is due to a [https://2009.igem.org/Team:LCG-UNAM-Mexico:BSD#BSD_using_the_Kamikaze_System modeling suggestion]. |
##Clone both colicines into any iGEM vector. | ##Clone both colicines into any iGEM vector. | ||
##Clone both colicines at the suffix of the biobrick BBa_R0010 in order to get an IPTG inducible device. | ##Clone both colicines at the suffix of the biobrick BBa_R0010 in order to get an IPTG inducible device. | ||
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Registry of the bioparts into the Registry of Standard Biological parts and in work in the wiki. :) | Registry of the bioparts into the Registry of Standard Biological parts and in work in the wiki. :) | ||
+ | |||
+ | |||
+ | '''References of the asRNA design''' | ||
+ | ---- | ||
+ | Antisense RNA directed against the major capsid protein of Lactococcus lactis subsp. cremoris bacteriophage confers partial resistance to the host | ||
+ | Dae Kyun Chung, Sung Kyun Chung and Carl A. Batt | ||
+ | Applied Microbiology and Biotechnology, Springer Berlin / Heidelberg, 0175-7598 1432-0614 | ||
+ | |||
+ | Antisense mRNA-Mediated Bacteriophage Resistance in Lactococcus lactis subsp. lactis | ||
+ | Sung Guk Kim and Carl A. Batt | ||
+ | Appl Environ Microbiol. 1991 April; 57(4): 1109-1113 | ||
+ | |||
+ | Engineering of the mRNA-interfering complementary RNA immune system against viral infection. | ||
+ | A Hirashima, S Sawaki, Y Inokuchi, and M Inouye | ||
+ | Proc Natl Acad Sci U S A. 1986 October; 83(20): 7726–7730. | ||
+ | |||
+ | Artificial Immune System against Viral Infection Involving Antisense RNA Targeted to the 5'-Terminal Noncoding Region of Coliphage SP RNA | ||
+ | The Journal of Biochemistry, Volume 106, Number 1, Pp. 163-166 | ||
+ | Akikazu Hiroshima, Saeko Sawaki, Takafumi Mizuno, Nicole Houba-Herin and Masayori Inouye | ||
+ | |||
+ | An Explosive Antisense RNA Strategy for Inhibition of a Lactococcal | ||
+ | Applied and Environmental Microbiology, January 2000, p. 310-319, Vol. 66, No. 1 | ||
+ | 0099-2240/0/$04.00+0 | ||
+ | |||
+ | Bacteriophage T7 gene 2.5 protein: An essential protein for | ||
+ | DNA replication | ||
+ | Proc. Natl. Acad. Sci. USA Vol. 90, pp. 10173-10177, November 1993 Biochemistry | ||
+ | Young Tae Kim and Charles C. Richardson | ||
+ | |||
+ | |||
+ | Acidic Carboxyl-terminal Domain of Gen2e. 5 Protein of | ||
+ | Bacteriophage T7 Is Essential for Protein-Protein Interactions* | ||
+ | Young Tae Kim4 and Charles C. Richardson5 | ||
+ | Vol. 269, No. 7, Issue of February 18, pp. 5270-5278, 1994 Printed in U.S.A. | ||
+ | |||
+ | Essential Residues in the C Terminus of the Bacteriophage T7 Gene 2.5 Single-stranded DNA-binding Protein* | ||
+ | Boriana Marintchev,. Samir M. Hamdan,. Seung-Joo Lee and. Charles C. Richardson3 | ||
+ | 28, 2006, doi: 10.1074/jbc | ||
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Latest revision as of 03:14, 22 October 2009