Team:Nevada/Team

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Advisors

• Advisor 1: Dr. Christie Howard

• Advisor 2: Dr. Patricia Ellison

• Advisor 3: Dr. Dave Shintani

• Grad Student 1: Chad Sanada

Undergrads

• Student 1: Nick Tschernia

• Student 2: Tony Moschetti

• Student 3: Joey Khwaja

• Student 4: Chris Martinez

• Student 5: Janice Cho

• Student 6: Leigh Armijo

• Student 7: Sheena Jones

• Student 8: Tyler Peterson

• Student 9: J Malikowski

• Student 10: Alex Dussaq

Nick Tschernia

• Entered the Nevada Team into the iGEM Competition a week before the submission date.
• Organized weekly meetings for project brainstorming and timeline.
• Recruited the iGEM Team through a highly selective process (whoever was left at the end of the brainstorming sessions).
• Organized the source plate distribution and transformation of the basic parts.
• Sequence analysis of the Biobrick parts formed.
• Primer design for adding Biobrick restriction sites for formation of Biobrick parts.

Janice Cho

• Formulated the original idea of using cinnamaldehyde as a mosquito larvicide.
• Performed initial research on the use of cinnamaldehyde.
• Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.
• Helped conduct a three-way ligation to create final construct with the parts: BBa_K262000 and BBa_K262002.

Leigh Armijo

• Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.
• Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014.

Sheena Jones

• In charge of maintaining and propagating Wolffia and Lemna plant species.
• Generated callus culture in preparation for gene transformation.

Tony Moschetti

• Topo-cloned cinnamoyl-CoA reductase (gene 3)for preparation of a Biobrick part.
• Made Biobrick part containing cinnamoyl-CoA reductase (gene 3) and double terminator (BBa_K262002)
• Transformed 4-coumarate-CoA ligase (gene 2) and cinnamoyl-CoA reductase (gene 3 - BBa_K262001) into Biobrick backbones.

J Malikowski

• Induced expression in non-biobrick expression vector containing 4-coumarate-CoA ligase (gene 2). Detection of product formation at 311 nm. Further studies will be conducted in the near future.
• Helped to troubleshoot minipreps and other protocols. Resident expert on minipreps.
• Trained Tyler to be an excellent Molecular Biologist.
• Advisory role at lab meetings (How does the Harper lab do it?)

Tyler Peterson

• Major player in media preparation, agarose gels, stock solutions
• Helped with minipreps and E. coli transformation.

Chris Martinez

• Made db3.1 and NEB10 competent cells.
• Overall goal was to upregulate the cinnamaldehyde pathway in plants Wolffia and various Lemna species
• Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector
• Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants Wolffia and Lemna species

Joey Khwaja

• Made db3.1 and NEB10 competent cells.
• Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector
• Assisted others on various projects including Gene2/Terminator part creation and attempts of the final construct 3-way ligation.
• Researched and began design of a Biobrick plant transformation binary vector.
• Designed the iGEM Nevada Team shirts.

Chad Sanada

• Assisted Nick with designing biobrick compatible primers and helped to direct and organize the amplification and cloning of cinnamoyl-CoA Reductase (Gene 3) in order to make our first biobrick part.
• Added insight into experimental methods and approaches when possible.
• Helped prevent Nick from going insane.
• Assisted with reviewing and editing the team wiki site.