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Team:Nevada/Team
From 2009.igem.org
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[[Nick Tschernia]] | [[Nick Tschernia]] | ||
* Entered the Nevada Team into the iGEM Competition a week before the submission date. | * Entered the Nevada Team into the iGEM Competition a week before the submission date. | ||
| - | * Organized weekly meetings for project brainstorming and | + | * Organized weekly meetings for project brainstorming and time line. |
* Recruited the iGEM Team through a highly selective process (whoever was left at the end of the brainstorming sessions). | * Recruited the iGEM Team through a highly selective process (whoever was left at the end of the brainstorming sessions). | ||
| + | * Helped petition for research and travel funds. | ||
* Organized the source plate distribution and transformation of the basic parts. | * Organized the source plate distribution and transformation of the basic parts. | ||
* Sequence analysis of the Biobrick parts formed. | * Sequence analysis of the Biobrick parts formed. | ||
* Primer design for adding Biobrick restriction sites for formation of Biobrick parts. | * Primer design for adding Biobrick restriction sites for formation of Biobrick parts. | ||
| + | * Assisted in an advisory role to all aspects of the project. | ||
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* Formulated the original idea of using cinnamaldehyde as a mosquito larvicide. | * Formulated the original idea of using cinnamaldehyde as a mosquito larvicide. | ||
* Performed initial research on the use of cinnamaldehyde. | * Performed initial research on the use of cinnamaldehyde. | ||
| - | * Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 ( | + | * Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribosome-binding site), and part pSB3C5 (chloramphenicol resistant backbone) to create part BBa_K262000. |
* Helped conduct a three-way ligation to create final construct with the parts: BBa_K262000 and BBa_K262002. | * Helped conduct a three-way ligation to create final construct with the parts: BBa_K262000 and BBa_K262002. | ||
[[Leigh Armijo]] | [[Leigh Armijo]] | ||
| - | * Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 ( | + | * Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribosome-binding site), and part pSB3C5 (chloramphenicol resistant backbone) to create part BBa_K262000. |
* Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014. | * Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014. | ||
[[Sheena Jones]] | [[Sheena Jones]] | ||
| - | * In charge of maintaining and propagating ''Wolffia'' and ''Lemna'' plant species. | + | * In charge of maintaining and propagating ''Wolffia'' and ''Lemna Minor'' plant species. |
* Generated callus culture in preparation for gene transformation. | * Generated callus culture in preparation for gene transformation. | ||
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[[J Malikowski]] | [[J Malikowski]] | ||
| - | * Induced expression in non- | + | * Induced expression in non-Biobrick expression vector containing 4-coumarate-CoA ligase (gene 2). Detection of product formation at 311nm. Further studies will be conducted in the near future. |
* Helped to troubleshoot minipreps and other protocols. Resident expert on minipreps. | * Helped to troubleshoot minipreps and other protocols. Resident expert on minipreps. | ||
* Trained Tyler to be an excellent Molecular Biologist. | * Trained Tyler to be an excellent Molecular Biologist. | ||
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[[Chris Martinez]] | [[Chris Martinez]] | ||
* Made db3.1 and NEB10 competent cells. | * Made db3.1 and NEB10 competent cells. | ||
| - | * Overall goal was to | + | * Overall goal was to up-regulate the cinnamaldehyde pathway in plants ''Wolffia'' and various ''Lemna Minor'' species |
* Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector | * Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector | ||
| - | * Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants ''Wolffia'' and ''Lemna'' species | + | * Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants ''Wolffia'' and ''Lemna Minor'' species |
[[Joey Khwaja]] | [[Joey Khwaja]] | ||
| - | * Made db3.1 and NEB10 competent cells. | + | * Made db3.1 and NEB10 competent cells. |
| - | * | + | * Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector |
| + | * Assisted others on various projects including Gene2/Terminator part creation and attempts of the final construct 3-way ligation. | ||
| + | * Researched and began design of a Biobrick plant transformation binary vector. | ||
* Designed the iGEM Nevada Team shirts. | * Designed the iGEM Nevada Team shirts. | ||
| + | |||
| + | |||
| + | [[Chad Sanada]] | ||
| + | * Assisted Nick with designing Biobrick compatible primers and helped to direct and organize the amplification and cloning of cinnamoyl-CoA Reductase (Gene 3) in order to make our first biobrick part. | ||
| + | * Added insight into experimental methods and approaches when possible. | ||
| + | * Helped prevent Nick from going insane. | ||
| + | * Assisted with reviewing and editing the team wiki site. | ||
</div> | </div> | ||
| + | |||
== '''Meet the Team''' == | == '''Meet the Team''' == | ||
| - | + | <html> | |
| - | < | + | <table style="margin-left:50px;margin-top:35px;height:auto;"> |
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| - | + | <td colspan="4"> | |
| + | </html> | ||
| + | ==Undergraduates== | ||
| + | <html> | ||
| + | </td> | ||
| + | </tr> | ||
| + | <tr> | ||
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| + | </html> | ||
[[Image:Nick.jpg|left|thumb|alt=alt text|Nick Tschernia]] | [[Image:Nick.jpg|left|thumb|alt=alt text|Nick Tschernia]] | ||
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| + | </td> | ||
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| + | </html> | ||
[[Image:Tony.jpg|left|thumb|alt=alt text|Tony Moschetti]] | [[Image:Tony.jpg|left|thumb|alt=alt text|Tony Moschetti]] | ||
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[[Image:Janice.jpg|left|thumb|alt=alt text|Janice Cho]] | [[Image:Janice.jpg|left|thumb|alt=alt text|Janice Cho]] | ||
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| + | </td> | ||
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[[Image:Leigh.jpg|left|thumb|alt=alt text|Leigh Armijo]] | [[Image:Leigh.jpg|left|thumb|alt=alt text|Leigh Armijo]] | ||
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| + | </td> | ||
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[[Image:Khwaja.jpg|left|thumb|alt=alt text|Joey Khwaja]] | [[Image:Khwaja.jpg|left|thumb|alt=alt text|Joey Khwaja]] | ||
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| + | </td> | ||
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[[Image:Sheena.JPG|left|thumb|alt=alt text|Sheena Jones]] | [[Image:Sheena.JPG|left|thumb|alt=alt text|Sheena Jones]] | ||
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| + | </td> | ||
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[[Image:Chris2.jpg|left|thumb|alt=alt text|Chris Martinez]] | [[Image:Chris2.jpg|left|thumb|alt=alt text|Chris Martinez]] | ||
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| + | </td> | ||
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[[Image:tyler.jpg|left|thumb|alt=alt text|Tyler Peterson]] | [[Image:tyler.jpg|left|thumb|alt=alt text|Tyler Peterson]] | ||
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[[Image:j.jpg|left|thumb|alt=alt text|J Malikowski]] | [[Image:j.jpg|left|thumb|alt=alt text|J Malikowski]] | ||
| + | <html> | ||
| + | </td> | ||
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| + | </html> | ||
[[Image:Alex.jpg|left|thumb|alt=alt text|Alex Dussaq]] | [[Image:Alex.jpg|left|thumb|alt=alt text|Alex Dussaq]] | ||
| - | + | <html> | |
| - | + | </td> | |
| - | + | <td></td> | |
| - | + | <td></td> | |
| - | + | </tr> | |
| - | + | <tr> | |
| - | + | <td colspan="4"> | |
| - | + | </html> | |
| - | < | + | ==Advisors== |
| - | < | + | <html> |
| - | < | + | </td> |
| - | < | + | </tr> |
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[[Image:christie.jpg|left|thumb|alt=alt text|Dr. Christie Howard]] | [[Image:christie.jpg|left|thumb|alt=alt text|Dr. Christie Howard]] | ||
| - | [[Image:Shintani.jpg| | + | <html> |
| - | [[Image:Trish.jpeg| | + | </td> |
| - | </ | + | <td> |
| - | + | </html> | |
| - | + | [[Image:Shintani.jpg|left|thumb|alt=alt text|Dr. Dave Shintani]] | |
| + | <html> | ||
| + | </td> | ||
| + | <td> | ||
| + | </html> | ||
| + | [[Image:Trish.jpeg|left|thumb|alt=alt text|Dr. Trish Ellison]] | ||
| + | <html> | ||
| + | </td> | ||
| + | <td> | ||
| + | </html> | ||
| + | [[Image:chad.jpeg|left|thumb|alt=alt text|Chad Sanada (Grad Student)]] | ||
| + | <html> | ||
| + | </td> | ||
| + | </tr> | ||
| + | </table> | ||
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<div style="float:left;width:950px;height:35px;"></div> | <div style="float:left;width:950px;height:35px;"></div> | ||
Latest revision as of 03:24, 22 October 2009
Back: Dr. Shintani, J, Alex, Tyler. Middle: Janice, Leigh, Joey, Chris. Front: Nick and Dr. Howard
Contents |
Who we are
Advisors
- Advisor 1: Dr. Christie Howard
- Advisor 2: Dr. Patricia Ellison
- Advisor 3: Dr. Dave Shintani
- Grad Student 1: Chad Sanada
Undergrads
- Student 1: Nick Tschernia
- Student 2: Tony Moschetti
- Student 3: Joey Khwaja
- Student 4: Chris Martinez
- Student 5: Janice Cho
- Student 6: Leigh Armijo
- Student 7: Sheena Jones
- Student 8: Tyler Peterson
- Student 9: J Malikowski
- Student 10: Alex Dussaq
What we did
- Entered the Nevada Team into the iGEM Competition a week before the submission date.
- Organized weekly meetings for project brainstorming and time line.
- Recruited the iGEM Team through a highly selective process (whoever was left at the end of the brainstorming sessions).
- Helped petition for research and travel funds.
- Organized the source plate distribution and transformation of the basic parts.
- Sequence analysis of the Biobrick parts formed.
- Primer design for adding Biobrick restriction sites for formation of Biobrick parts.
- Assisted in an advisory role to all aspects of the project.
- Formulated the original idea of using cinnamaldehyde as a mosquito larvicide.
- Performed initial research on the use of cinnamaldehyde.
- Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribosome-binding site), and part pSB3C5 (chloramphenicol resistant backbone) to create part BBa_K262000.
- Helped conduct a three-way ligation to create final construct with the parts: BBa_K262000 and BBa_K262002.
- Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribosome-binding site), and part pSB3C5 (chloramphenicol resistant backbone) to create part BBa_K262000.
- Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014.
- In charge of maintaining and propagating Wolffia and Lemna Minor plant species.
- Generated callus culture in preparation for gene transformation.
- Topo-cloned cinnamoyl-CoA reductase (gene 3)for preparation of a Biobrick part.
- Made Biobrick part containing cinnamoyl-CoA reductase (gene 3) and double terminator (BBa_K262002)
- Transformed 4-coumarate-CoA ligase (gene 2) and cinnamoyl-CoA reductase (gene 3 - BBa_K262001) into Biobrick backbones.
- Induced expression in non-Biobrick expression vector containing 4-coumarate-CoA ligase (gene 2). Detection of product formation at 311nm. Further studies will be conducted in the near future.
- Helped to troubleshoot minipreps and other protocols. Resident expert on minipreps.
- Trained Tyler to be an excellent Molecular Biologist.
- Advisory role at lab meetings (How does the Harper lab do it?)
- Major player in media preparation, agarose gels, stock solutions
- Helped with minipreps and E. coli transformation.
- Made db3.1 and NEB10 competent cells.
- Overall goal was to up-regulate the cinnamaldehyde pathway in plants Wolffia and various Lemna Minor species
- Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector
- Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants Wolffia and Lemna Minor species
- Made db3.1 and NEB10 competent cells.
- Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector
- Assisted others on various projects including Gene2/Terminator part creation and attempts of the final construct 3-way ligation.
- Researched and began design of a Biobrick plant transformation binary vector.
- Designed the iGEM Nevada Team shirts.
- Assisted Nick with designing Biobrick compatible primers and helped to direct and organize the amplification and cloning of cinnamoyl-CoA Reductase (Gene 3) in order to make our first biobrick part.
- Added insight into experimental methods and approaches when possible.
- Helped prevent Nick from going insane.
- Assisted with reviewing and editing the team wiki site.
Meet the Team
Undergraduates
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Advisors
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