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| class="t">The Team</span></a> | | class="t">The Team</span></a> |
| <ul> | | <ul> |
- | <li><a href="#">Overview</a></li> | + | <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team">Overview</a></li> |
- | <li><a href="#">Photos</a></li> | + | <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team/Portraits">Portraits</a></li> |
| </ul> | | </ul> |
| </li> | | </li> |
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| href="https://2009.igem.org/Team:Freiburg_bioware/Project#Summary">Summary</a> | | href="https://2009.igem.org/Team:Freiburg_bioware/Project#Summary">Summary</a> |
| </li> | | </li> |
- | <li><a | + | |
- | href="https://2009.igem.org/Team:Freiburg_bioware/Project#Subprojects">Subprojects</a></li>
| + | |
| <li><a | | <li><a |
| href="https://2009.igem.org/Team:Freiburg_bioware/Project#Highlights">Highlights</a></li> | | href="https://2009.igem.org/Team:Freiburg_bioware/Project#Highlights">Highlights</a></li> |
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| class="t">Human | | class="t">Human |
| Practice</span></a> | | Practice</span></a> |
- | <ul>
| |
- | <li><a href="#">D'oh! Something's missing here.</a>
| |
| <ul> | | <ul> |
- | <li><a href="#">1</a> </li>
| + | <li><a |
- | <li><a href="#">2</a> </li>
| + | href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice/Ethics">Ethics</a> |
- | <li><a href="#">3</a> </li>
| + | |
- | </ul>
| + | |
| </li> | | </li> |
- | <li><a href="#">4</a></li> | + | <li><a |
- | <li><a href="#">5</a></li>
| + | href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice/Safety">Safety</a></li> |
| </ul> | | </ul> |
| + | |
| </li> | | </li> |
| <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Notebook"><span class="l"></span><span | | <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Notebook"><span class="l"></span><span |
| class="r"></span><span class="t">Notebook</span></a></li> | | class="r"></span><span class="t">Notebook</span></a></li> |
| <li><a | | <li><a |
- | href="https://2009.igem.org/Team:Freiburg_bioware/cloning" class="active"><span | + | href="https://2009.igem.org/Team:Freiburg_bioware/cloning1"class="active"><span |
| class="l"></span><span class="r"></span><span | | class="l"></span><span class="r"></span><span |
- | class="t">Parts</span></a></li> | + | class="t">Parts</span></a><ul> |
| + | <li><a |
| + | href="https://2009.igem.org/Team:Freiburg_bioware/cloning1">Basic |
| + | Parts</a></li> |
| + | <li><a |
| + | href="https://2009.igem.org/Team:Freiburg_bioware/cloning">Composite |
| + | Parts</a></li> |
| + | </ul></li> |
| <li><a | | <li><a |
| href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span | | href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span |
| class="l"></span><span class="r"></span><span | | class="l"></span><span class="r"></span><span |
| class="t">Collaboration</span></a></li> | | class="t">Collaboration</span></a></li> |
| + | <li><a |
| + | href="https://2009.igem.org/Team:Freiburg_bioware/Modeling"><span |
| + | class="l"></span><span class="r"></span><span |
| + | class="t">Modeling</span></a></li> |
| </ul> | | </ul> |
| </div> | | </div> |
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| <div class="art-Post-inner"> | | <div class="art-Post-inner"> |
| <div class="art-PostMetadataHeader"> | | <div class="art-PostMetadataHeader"> |
- | | + | <a name="1"></a> |
- | | + | <h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> Index<span |
- | <p></div><a name="1"></a>
| + | class="art-PostHeader"></span> </h2> |
- | <h3>Index</h3><br> | + | </div> |
- | | + | <div class="art-PostContent"> |
| + | <div style="text-align: left;"> |
| | | |
| <table border="0"> | | <table border="0"> |
| <tr><td> | | <tr><td> |
| <p><font size="2"> | | <p><font size="2"> |
- | *<a href="#2">His+FluA+Split+Foki_pMA | + | *<a href="#2">FokA_pMA |
| </a><br> | | </a><br> |
- | *<a href="#3">His+Dig+Split+FokA_pMA | + | *<a href="#3">Foki_pMA |
| </a><br> | | </a><br> |
- | *<a href="#4"> | + | *<a href="#4">Dig_pMA |
- | DsbA+His+Dig+Split+FokA_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#5"> | + | *<a href="#5">FluA_pMA |
- | Strep+FluA+ShortLinker+FokI_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#6"> | + | *<a href="#6">DsbA_pMA |
- | His+FluA+middellinker+FokA_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#7"> | + | *<a href="#7">StrepTag_pMA |
- | His+FluA+middellinker+Foki_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#8"> | + | *<a href="#8">HisTag_pMA |
- | His+FluA+shortlinker+FokA_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#9"> | + | *<a href="#9">shortlinker26_pMA |
- | His+FluA+shortlinker+Foki_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#10"> | + | *<a href="#10">Middellinker38_pMA |
- | His+Dig+longlinker+FokA_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#11"> | + | *<a href="#11">LongLinkerA50_pMA |
- | His+Dig+longlinker+Foki_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#12"> | + | *<a href="#12">GSAT_linker_pMA |
- | Strep+FluA+Split+Foki_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#13"> | + | *<a href="#13">SEG_Linker_pMA |
- | His+Dig+middellinker+FokA_pMA | + | |
| </a><br> | | </a><br> |
- | *<a href="#14"> | + | *<a href="#14">Split_Linker_pMA |
- | His+Dig+middellinker+Foki_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#15"> | + | *<a href="#15">YFP(Venus)_pGA14 |
- | His+Dig+shortlinker+FokA_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#16"> | + | *<a href="#19">Fos_pMA |
- | His+Dig+shortlinker+Foki_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#17"> | + | *<a href="#16">pJS418 Vector |
- | His+Dig+Split+Foki_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#18"> | + | *<a href="#17">pJS419 Vector |
- | His+FluA+longlinker+FokA_pMA
| + | |
| </a><br> | | </a><br> |
- | </p></font></p>
| + | *<a href="#18">pBAD Vector |
- | | + | |
- | </td>
| + | |
- | <td>
| + | |
- | <p><font size="2">
| + | |
- | *<a href="#19"> | + | |
- | His+FluA+longlinker+Foki_pMA
| + | |
| </a><br> | | </a><br> |
- | *<a href="#20"> | + | *<a href="#20">pEX Vector |
- | His+FluA+Split+FokA_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#21">
| + | |
- | Strep+Dig+longlinker+FokA_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#22">
| + | |
- | Strep+Dig+longlinker+Foki_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#23">
| + | |
- | Strep+Dig+middellinker+FokA_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#24">
| + | |
- | Strep+Dig+middellinker+Foki_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#25">
| + | |
- | Strep+FluA+Split+FokA_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#26">
| + | |
- | Strep+FluA+Split+FokA_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#27">
| + | |
- | Strep+Dig+shortlinker+FokA_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#28">
| + | |
- | Strep+Dig+shortlinker+Foki_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#29">
| + | |
- | Strep+Dig+Split+FokA_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#30">
| + | |
- | Strep+Dig+Split+Foki_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#31">
| + | |
- | Strep+FluA+longlinker+FokA_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#32">
| + | |
- | Strep+FluA+longlinker+Foki_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#33">
| + | |
- | Strep+FluA+middellinker+FokA_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#34">
| + | |
- | Strep+FluA+middellinker+Foki_pMA
| + | |
- | </a><br>
| + | |
- | *<a href="#35">
| + | |
- | Strep+FluA+shortlinker+FokA_pMA
| + | |
| </a><br> | | </a><br> |
| | | |
| </p></font></p> | | </p></font></p> |
- |
| |
- |
| |
| </td></tr></table> | | </td></tr></table> |
| | | |
| + | <li style=" text-align:center;"><a href="https://2009.igem.org/Team:Freiburg_bioware/oligos">list of oligonucleotides</a></li> |
| | | |
| | | |
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| | | |
| | | |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | <h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> Parts<span class="art-PostHeader"></span> </h2>
| |
| </div> | | </div> |
- | | + | <br /> |
- | | + | <div style="text-align: center;"> |
- | </div><a name="2"></a>
| + | </div> |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
| + | <br /> |
- | <div class="art-PostContent">
| + | </div> |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | + | <div class="cleared"></div> |
- | | + | |
- | | + | |
- | <table border="5"> | + | |
- | <tr>
| + | |
- | <td align="center">
| + | |
- | <img src="https://static.igem.org/mediawiki/2009/3/37/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.jpg" name="His+FluA+SplitLinker+Foki in pMA Vector" width="480" height="480" />
| + | |
- | <br><p align="center">His+FluA+SplitLinker+Foki in pMA Vector</p> | + | |
- | </td>
| + | |
- | <td align="left">
| + | |
- | nucleotid sequence of ORF-1:<br>
| + | |
- | | + | |
- | ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
| + | |
- | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| + | |
- | ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
| + | |
- | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| + | |
- | GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
| + | |
- | CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
| + | |
- | GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
| + | |
- | CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
| + | |
- | GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| + | |
- | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| + | |
- | CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
| + | |
- | ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA
| + | |
- | GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT
| + | |
- | GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA
| + | |
- | TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG
| + | |
- | TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG
| + | |
- | TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG
| + | |
- | TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT
| + | |
- | CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA
| + | |
- | ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG
| + | |
- | AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC
| + | |
- | CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG
| + | |
- | TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA
| + | |
- | CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC
| + | |
- | GGTTAA
| + | |
- | </td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <h4>AA sequence of ORF-1:<br></h4>
| + | |
- | <table border="0">
| + | |
- | <p align="justify"><td align="left">
| + | |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP<br>
| + | |
- | EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY<br>
| + | |
- | IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA<br>
| + | |
- | CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ<br>
| + | |
- | DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG<br>
| + | |
- | QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN<br>
| + | |
- | SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG<br><br></p></td></table>
| + | |
- | | + | |
- | <h4>Commented GenBank file</h4>
| + | |
- | <li> <a href="https://static.igem.org/mediawiki/2009/d/d8/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.txt">His+FluA+Split+Foki_pMA.txt</a></li>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | </div><a name="3"></a> | + | |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
| + | |
- | | + | |
- | <div class="art-PostContent"> | + | |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| + | |
- | | + | |
- | <table border="5">
| + | |
- | <tr>
| + | |
- | <td align="center">
| + | |
- | <img src="https://static.igem.org/mediawiki/2009/3/34/Freiburg09_His%2BDig%2BSplit%2BFokA_pMA.JPG" name="His+Dig+Split+FokA in pMA Vector" width="480" height="480" />
| + | |
- | <br><p align="center">His+Dig+Split+FokA in pMA Vector</p>
| + | |
- | </td>
| + | |
- | <td align="left">
| + | |
- | nucleotid sequence of ORF-1:<br>
| + | |
- | | + | |
- | ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
| + | |
- | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| + | |
- | ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
| + | |
- | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| + | |
- | GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
| + | |
- | CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
| + | |
- | ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
| + | |
- | CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
| + | |
- | GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| + | |
- | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| + | |
- | CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
| + | |
- | ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC
| + | |
- | TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA
| + | |
- | ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG
| + | |
- | TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA
| + | |
- | GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG
| + | |
- | CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT
| + | |
- | ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC
| + | |
- | TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG
| + | |
- | TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT
| + | |
- | GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG
| + | |
- | AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA
| + | |
- | TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG
| + | |
- | GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT
| + | |
- | AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| + | |
- | | + | |
- | </td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <h4>AA sequence of ORF-1:<br></h4>
| + | |
- | <table border="0">
| + | |
- | <p align="justify"><td align="left">
| + | |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
| + | |
- | EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
| + | |
- | IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| + | |
- | CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE
| + | |
- | LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA
| + | |
- | YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA
| + | |
- | QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | <br><br></p></td></table>
| + | |
- | | + | |
- | <h4>Commented GenBank file</h4>
| + | |
- | <li> <a href="https://static.igem.org/mediawiki/2009/d/dd/Freiburg09_His%2BDig%2BSplit%2BFokA_pMA.txt">His+Dig+Split+FokA_pMA.txt</a></li>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | </div><a name="4"></a> | + | |
- | <h3 class="art-PostHeaderIcon-wrapper"> DsbA+His+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
| + | |
| </div> | | </div> |
- | <div class="art-PostContent">
| |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
- |
| |
- |
| |
- | <table border="5">
| |
- | <tr>
| |
- | <td align="center">
| |
- | <img src="https://static.igem.org/mediawiki/2009/4/4f/Freiburg09_DsbA%2BHis%2BDig%2BSplit%2BFokA_pMA.JPG" name="DsbA+His+Dig+Split+FokA in pMA Vector" width="480" height="480" />
| |
- | <br><p align="center">DsbA+His+Dig+Split+FokA in pMA Vector</p>
| |
- | </td>
| |
- | <td align="left">
| |
- | nucleotid sequence of ORF-1:<br>
| |
- |
| |
- | ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC
| |
- | ATCGGCGACCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACG
| |
- | ACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAG
| |
- | TACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAA
| |
- | GTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGA
| |
- | AGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGC
| |
- | ACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTA
| |
- | CACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCG
| |
- | ACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAG
| |
- | AAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGAC
| |
- | CGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGG
| |
- | TGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAA
| |
- | GTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACC
| |
- | AGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCG
| |
- | GCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAA
| |
- | TATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTAC
| |
- | CCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCT
| |
- | ATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCC
| |
- | ATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAA
| |
- | AGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGC
| |
- | AGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAAC
| |
- | GAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTT
| |
- | CGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGA
| |
- | ACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTG
| |
- | ATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCG
| |
- | CCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| |
- |
| |
- |
| |
- | </td>
| |
- | </tr>
| |
- | </table>
| |
- | <br>
| |
- | <h4>AA sequence of ORF-1:<br></h4>
| |
- | <table border="0">
| |
- | <p align="justify"><td align="left">
| |
- | MAGMKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA
| |
- | YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG
| |
- | GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG
| |
- | SPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEE
| |
- | KKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGA
| |
- | IYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSV
| |
- | TEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKF
| |
- | NNGEINFTG
| |
- |
| |
- | <br><br></p></td></table>
| |
- |
| |
- | <h4>Commented GenBank file</h4>
| |
- | <li> <a href="https://static.igem.org/mediawiki/2009/4/4d/Freiburg09_DsbA%2BHis%2BDig%2BSplit%2BFokA_pMA.txt">DsbA+His+Dig+Split+FokA_pMA.txt</a></li>
| |
- | <br>
| |
- | <br>
| |
- | <br>
| |
- | </div><a name="5"></a>
| |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+ShortLinker+FokI_pMA<span class="art-PostHeader"></span> </h3>
| |
| </div> | | </div> |
- | <div class="art-PostContent">
| |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
- |
| |
- |
| |
- | <table border="5">
| |
- | <tr>
| |
- | <td align="center">
| |
- | <img src="https://static.igem.org/mediawiki/2009/0/03/Freiburg09_Strep%2BFluA%2BShortLinker%2BFokI_pMA.jpg" name="Strep+FluA+ShortLinker+FokI in pMA Vector" width="480" height="480" />
| |
- | <br><p align="center">Strep+FluA+ShortLinker+FokI in pMA Vector</p>
| |
- | </td>
| |
- | <td align="left">
| |
- | nucleotid sequence of ORF-1:<br>
| |
- |
| |
- | ATGGCC
| |
- | GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
| |
- | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| |
- | ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
| |
- | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| |
- | GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
| |
- | CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
| |
- | GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
| |
- | CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
| |
- | GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| |
- | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| |
- | CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
| |
- | ACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAA
| |
- | AAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGA
| |
- | ACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGA
| |
- | AAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTG
| |
- | GGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGAT
| |
- | CGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATC
| |
- | TGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAG
| |
- | ACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTC
| |
- | AAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCA
| |
- | ACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGG
| |
- | GCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGC
| |
- | AGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGA
| |
- | TCAATTTTACCGGTTAA
| |
- |
| |
- |
| |
- |
| |
- | </td>
| |
- | </tr>
| |
- | </table>
| |
- | <br>
| |
- | <h4>AA sequence of ORF-1:<br></h4>
| |
- | <table border="0">
| |
- | <p align="justify"><td align="left">
| |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
| |
- | EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
| |
- | IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| |
- | CKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM
| |
- | KVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQ
| |
- | TRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLI
| |
- | GGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
- |
| |
- |
| |
- | <br><br></p></td></table>
| |
- | <h4>Commented GenBank file</h4>
| |
- | <li> <a href="https://static.igem.org/mediawiki/2009/7/7e/Freiburg09_Strep%2BFluA%2BShortLinker%2BFokI_pMA.txt">Strep+FluA+ShortLinker+FokI_pMA.txt</a></li>
| |
- | <br>
| |
- | <br>
| |
- | <br>
| |
- |
| |
- | </div><a name="6"></a>
| |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
| </div> | | </div> |
- | <div class="art-PostContent">
| |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| | | |
| | | |
- | <table border="5">
| |
- | <tr>
| |
- | <td align="center">
| |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/a/ad/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg" name="His+FluA+middellinker+FokA in pMA Vector" width="480" height="480" />
| |
- | <br><p align="center">His+FluA+middellinker+FokA in pMA Vector</p>
| |
- | </td>
| |
- | <td align="left">
| |
- | nucleotid sequence of ORF-1:<br>
| |
| | | |
- | ATGGCCGGC
| + | <div class="art-contentLayout"> |
- | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| + | <div class="art-content"> |
- | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| + | <div class="art-Post"> |
- | GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
| + | <div class="art-Post-tl"></div> |
- | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
| + | <div class="art-Post-tr"></div> |
- | CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
| + | <div class="art-Post-bl"></div> |
- | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
| + | <div class="art-Post-br"></div> |
- | TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
| + | <div class="art-Post-tc"></div> |
- | CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
| + | <div class="art-Post-bc"></div> |
- | ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| + | <div class="art-Post-cl"></div> |
- | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| + | <div class="art-Post-cr"></div> |
- | ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
| + | <div class="art-Post-cc"></div> |
- | GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG
| + | <div class="art-Post-body"> |
- | AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT
| + | <div class="art-Post-inner"> |
- | CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA
| + | <div class="art-PostMetadataHeader"> |
- | TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT
| + | |
- | CTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCC
| + | |
- | GATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATA
| + | |
- | ACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAAC
| + | |
- | CAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCC
| + | |
- | GTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAG
| + | |
- | GCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAAT
| + | |
- | GGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAA
| + | |
- | AGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGG
| + | |
- | AGATCAATTTTACCGGTTAA
| + | |
| | | |
| | | |
- | | + | <h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> Parts<span |
- | </td> | + | class="art-PostHeader"></span> </h2> |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <h4>AA sequence of ORF-1:<br></h4>
| + | |
- | <table border="0">
| + | |
- | <p align="justify"><td align="left">
| + | |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
| + | |
- | SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
| + | |
- | YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| + | |
- | NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF
| + | |
- | MKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKEN
| + | |
- | QTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELL
| + | |
- | IGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | <br><br></p></td></table>
| + | |
- | | + | |
- | <h4>Commented GenBank file</h4>
| + | |
- | <li> <a href="https://static.igem.org/mediawiki/2009/7/78/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.txt">His+FluA+middellinker+FokA_pMA.txt</a></li>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | </div><a name="7"></a>
| + | |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| + | |
| </div> | | </div> |
- | <div class="art-PostContent">
| |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| | | |
- |
| |
- | <table border="5">
| |
- | <tr>
| |
- | <td align="center">
| |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/c/ce/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg" name="His+FluA+middellinker+Foki in pMA Vector" width="480" height="480" />
| |
- | <br><p align="center">His+FluA+middellinker+Foki in pMA Vector</p><br>
| |
- | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243014">link to part registry</a>
| |
- | </td>
| |
- | <td align="left">
| |
- | nucleotid sequence of ORF-1:<br>
| |
- |
| |
- | ATGGCCGGC
| |
- | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
- | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
- | GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
| |
- | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
| |
- | CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
| |
- | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
| |
- | TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
| |
- | CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
| |
- | ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
- | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
- | ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
| |
- | GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG
| |
- | AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT
| |
- | CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA
| |
- | TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT
| |
- | CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC
| |
- | GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA
| |
- | ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT
| |
- | CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC
| |
- | GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG
| |
- | GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT
| |
- | GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA
| |
- | AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG
| |
- | AGATCAATTTTACCGGTTAA
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | </td>
| |
- | </tr>
| |
- | </table>
| |
- | <br>
| |
- | <h4>AA sequence of ORF-1:<br></h4>
| |
- | <table border="0">
| |
- | <p align="justify"><td align="left">
| |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
| |
- | SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
| |
- | YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| |
- | NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF
| |
- | MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN
| |
- | QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL
| |
- | IGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
- |
| |
- |
| |
- |
| |
- | <br><br></p></td></table>
| |
- |
| |
- | <h4>Commented GenBank file</h4>
| |
- | <li> <a href="https://static.igem.org/mediawiki/2009/9/92/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.txt">His+FluA+middellinker+Foki_pMA.txt</a></li>
| |
- | <br>
| |
- | <br>
| |
- | <br>
| |
- |
| |
- | </div><a name="8"></a>
| |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
- | </div>
| |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | + | <div style="text-align: center;"> |
| | | |
| | | |
- | <table border="5">
| + | <a name="2"></a> |
- | <tr>
| + | <h3 class="art-PostHeaderIcon-wrapper"> FokA_pMA<span class="art-PostHeader"></span> </h3> |
- | <td align="center">
| + | |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/d/d4/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.jpg" name="His+FluA+shortlinker+FokA in pMA Vector" width="480" height="480" />
| + | |
- | <br><p align="center">His+FluA+shortlinker+FokA in pMA Vector</p>
| + | |
- | </td>
| + | |
- | <td align="left">
| + | |
- | nucleotid sequence of ORF-1:<br>
| + | |
- | | + | |
- | ATGGCCGGC
| + | |
- | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| + | |
- | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| + | |
- | GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
| + | |
- | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
| + | |
- | CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
| + | |
- | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
| + | |
- | TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
| + | |
- | CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
| + | |
- | ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| + | |
- | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| + | |
- | ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
| + | |
- | GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG
| + | |
- | CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA
| + | |
- | GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG
| + | |
- | AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC
| + | |
- | CGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG
| + | |
- | CGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTG
| + | |
- | GTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAAC
| + | |
- | AAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTAC
| + | |
- | AGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAG
| + | |
- | CACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTG
| + | |
- | TCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCT
| + | |
- | GACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTA
| + | |
- | CCGGTTAA
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | </td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <h4>AA sequence of ORF-1:<br></h4>
| + | |
- | <table border="0">
| + | |
- | <p align="justify"><td align="left">
| + | |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
| + | |
- | SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
| + | |
- | YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| + | |
- | NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY
| + | |
- | GYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRN
| + | |
- | KHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGE
| + | |
- | MIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | <br><br></p></td></table>
| + | |
- | | + | |
- | <h4>Commented GenBank file</h4>
| + | |
- | <li> <a href="https://static.igem.org/mediawiki/2009/4/46/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.txt">His+FluA+shortlinker+FokA_pMA.txt</a></li>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | </div><a name="9"></a>
| + | |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3> | + | |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
| | | |
- | <table border="5"> | + | <table border="5" style= "width: 100%;"> |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/b/bc/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.jpg" name="His+FluA+shortlinker+Foki in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/d/d9/Freiburg09_FokA_pMA.jpg/600px-Freiburg09_FokA_pMA.jpg" name="FokA in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">His+FluA+shortlinker+Foki in pMA Vector</p><br> | + | <br><p align="center">FokA in pMA Vector</p> |
- | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243013">link to part registry</a> | + | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243000">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
| | | |
- | ATGGCCGGC
| + | ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA |
- | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| + | ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA |
- | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| + | ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG |
- | GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
| + | AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGA |
- | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
| + | TGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTG |
- | CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
| + | ATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGAT |
- | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
| + | GAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAA |
- | TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
| + | CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAAT |
- | CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
| + | TCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACC |
- | ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| + | CGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGA |
- | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| + | GCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAG |
- | ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
| + | AAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA |
- | GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG
| + | |
- | CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA
| + | |
- | GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG
| + | |
- | AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC
| + | |
- | CGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG
| + | |
- | CGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTG
| + | |
- | GTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAAC
| + | |
- | AAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCAC
| + | |
- | CGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAG
| + | |
- | CCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTG
| + | |
- | AGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCT
| + | |
- | GACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTA
| + | |
- | CCGGTTAA
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| </td> | | </td> |
Line 762: |
Line 239: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
| + | MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL |
- | SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
| + | GGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNE |
- | YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| + | WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTL |
- | NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY
| + | TLEEVRRKFNNGEINFTG |
- | GYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRN
| + | |
- | KHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGE
| + | |
- | MIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
| <br><br></p></td></table> | | <br><br></p></td></table> |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/7/7d/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.txt">His+FluA+shortlinker+Foki_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/3/3a/Freiburg09_FokA_pMA.txt">FokA_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
| <br> | | <br> |
| | | |
- | </div><a name="10"></a>
| |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
- | </div>
| |
- | <div class="art-PostContent">
| |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| | | |
| | | |
- | <table border="5">
| + | <a name="3"></a> |
- | <tr>
| + | <h3 class="art-PostHeaderIcon-wrapper"> Foki_pMA<span class="art-PostHeader"></span> </h3> |
- | <td align="center">
| + | |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/7/7d/Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.jpg" name="His+Dig+longlinker+FokA in pMA Vector" width="480" height="480" />
| + | |
- | <br><p align="center">His+Dig+longlinker+FokA in pMA Vector</p>
| + | |
- | </td>
| + | |
- | <td align="left">
| + | |
- | nucleotid sequence of ORF-1:<br>
| + | |
- | | + | |
- | ATGGCCGGC
| + | |
- | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| + | |
- | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| + | |
- | GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
| + | |
- | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
| + | |
- | CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
| + | |
- | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
| + | |
- | GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
| + | |
- | CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
| + | |
- | ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| + | |
- | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| + | |
- | ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
| + | |
- | ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
| + | |
- | TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC
| + | |
- | CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
| + | |
- | TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
| + | |
- | ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
| + | |
- | TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
| + | |
- | ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
| + | |
- | ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
| + | |
- | TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
| + | |
- | TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
| + | |
- | AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
| + | |
- | TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
| + | |
- | CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
| + | |
- | TTTACCGGTTAA
| + | |
- | | + | |
- | </td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <h4>AA sequence of ORF-1:<br></h4>
| + | |
- | <table border="0">
| + | |
- | <p align="justify"><td align="left">
| + | |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
| + | |
- | SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
| + | |
- | YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| + | |
- | NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
| + | |
- | TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
| + | |
- | IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
| + | |
- | TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
- | <br><br></p></td></table>
| + | |
- | | + | |
- | <h4>Commented GenBank file</h4>
| + | |
- | <li> <a href="https://static.igem.org/mediawiki/2009/0/07/Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.txt">His+Dig+longlinker+FokA_pMA.txt</a></li>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | </div><a name="11"></a>
| + | |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3> | + | |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
| | | |
- | <table border="5"> | + | <table border="5" style= "width: 100%;"> |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/6/64/Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.JPG/600px-Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.JPG" name="His+Dig+longlinker+Foki in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/6/68/Freiburg09_Foki_pMA.jpg/600px-Freiburg09_Foki_pMA.jpg" name="Foki in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">His+Dig+longlinker+Foki in pMA Vector</p> | + | <br><p align="center">Foki in pMA Vector</p> |
| + | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243001">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
| | | |
- | ATGGCCGGC
| + | ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA |
- | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| + | ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA |
- | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| + | ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG |
- | GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
| + | AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC |
- | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
| + | AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG |
- | CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
| + | CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT |
- | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
| + | GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA |
- | GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
| + | CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT |
- | CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
| + | TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT |
- | ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| + | CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA |
- | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| + | ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG |
- | ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
| + | AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA |
- | ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
| + | |
- | TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC
| + | |
- | CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
| + | |
- | TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
| + | |
- | ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
| + | |
- | TAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
| + | |
- | ATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCG
| + | |
- | ATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCG
| + | |
- | TAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTG
| + | |
- | TCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTAT
| + | |
- | AAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGT
| + | |
- | GCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCA
| + | |
- | CCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAAT
| + | |
- | TTTACCGGTTAA
| + | |
| | | |
| | | |
Line 900: |
Line 290: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
| + | MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL |
- | SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
| + | GGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNE |
- | YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| + | WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTL |
- | NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
| + | TLEEVRRKFNNGEINFTG |
- | TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLP
| + | |
- | IGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHI
| + | |
- | TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| <br><br></p></td></table> | | <br><br></p></td></table> |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/e/ef/Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.txt">His+Dig+longlinker+Foki_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/b/b8/Freiburg09_Foki_pMA.txt">Foki_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
Line 920: |
Line 304: |
| | | |
| | | |
- | </div><a name="12"></a>
| + | |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+Split+Foki_pMA<span class="art-PostHeader"></span> </h3> | + | <a name="4"></a> |
- | </div>
| + | <h3 class="art-PostHeaderIcon-wrapper"> Dig_pMA<span class="art-PostHeader"></span> </h3> |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
| | | |
- | <table border="5"> | + | <table border="5" style= "width: 100%;"> |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/f/fa/Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.jpg" name="Strep+FluA+Split+Foki in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/8/84/Freiburg09_Dig_pMA.jpg/600px-Freiburg09_Dig_pMA.jpg" name="Dig in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">Strep+FluA+Split+Foki in pMA Vector</p> | + | <br><p align="center">Dig in pMA Vector</p> |
| + | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243003">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
| | | |
- | ATGGCC
| + | ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT |
- | GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
| + | GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGCAGGTGGCCG |
- | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| + | CTTATCCCGACCACATCACCAAGTACGGCAAGTGCGGCTGGGCCGAGTAC |
- | ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
| + | ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTACAGCGTGATCCACGG |
- | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| + | CAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTGTGGGCGACAGCAAGA |
- | GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
| + | TCGGCAAGATCTACCACAGCTACACCATCGGCGGCGTGACCCAGGTGGGC |
- | CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
| + | GTGAGCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACTT |
- | GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
| + | TTGCAGATACGACGAGGACAAGAAGGGCCACTGGGACGCCGTGTGGGTGC |
- | CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
| + | TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC |
- | GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| + | TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA |
- | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| + | CTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCAACTGGTCCCACCCCC |
- | CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
| + | AGTTCGAAAAGACCGGTTAA |
- | ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA
| + | |
- | GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT
| + | |
- | GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA
| + | |
- | TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG
| + | |
- | TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG
| + | |
- | TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG
| + | |
- | TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT
| + | |
- | CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA
| + | |
- | ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG
| + | |
- | AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC
| + | |
- | CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG
| + | |
- | TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA
| + | |
- | CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC
| + | |
- | GGTTAA
| + | |
| | | |
| | | |
Line 972: |
Line 343: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
| + | MAGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGKSVKVS |
- | EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
| + | RYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIGYFCRY |
- | IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| + | DEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNSNW |
- | CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
| + | SHPQFEKTG |
- | DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
| + | |
- | QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
| + | |
- | SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| <br><br></p></td></table> | | <br><br></p></td></table> |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/8/8d/Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.txt">Strep+FluA+Split+Foki_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/3/3d/Freiburg09_Dig_pMA.txt">Dig_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
| <br> | | <br> |
| | | |
- | | + | <a name="5"></a> |
- | </div><a name="13"></a>
| + | <h3 class="art-PostHeaderIcon-wrapper"> FluA_pMA<span class="art-PostHeader"></span> </h3> |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3> | + | |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
| | | |
- | <table border="5"> | + | <table border="5" style= "width: 100%;"> |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/c/c7/Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.jpg" name="His+Dig+middellinker+FokA in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/2/2a/Freiburg09_FluA_pMA.jpg/600px-Freiburg09_FluA_pMA.jpg" name="FluA in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">His+Dig+middellinker+FokA in pMA Vector</p> | + | <br><p align="center">FluA in pMA Vector</p> |
| + | <li> <a href="http://partsregistry.org/Part:BBa_K157004">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
| | | |
- | ATGGCCGGC
| + | ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT |
- | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| + | GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCA |
- | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| + | AGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTAC |
- | GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
| + | ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGG |
- | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
| + | CAAAGAATACTTCATGGAAGGCACCGCCTACCCCGTGGGCGACAGCAAGA |
- | CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
| + | TCGGCAAGATCTACCACAGCCGGACCGTGGGCGGCTACACCAGAAAGACC |
- | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
| + | GTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACAG |
- | GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
| + | CTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGC |
- | CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
| + | TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC |
- | ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| + | TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA |
- | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| + | CTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCGGTTAA |
- | ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
| + | |
- | ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
| + | |
- | TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
| + | |
- | CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
| + | |
- | GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
| + | |
- | ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
| + | |
- | TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
| + | |
- | TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT
| + | |
- | GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT
| + | |
- | CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA
| + | |
- | AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG
| + | |
- | ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA
| + | |
- | AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG
| + | |
- | AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| + | |
| | | |
| | | |
Line 1,045: |
Line 394: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
| + | MAGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGKSVKVS |
- | SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
| + | RYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIGYSCRY |
- | YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| + | DEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNTG |
- | NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
| + | |
- | ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA
| + | |
- | DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN
| + | |
- | GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| | | |
Line 1,061: |
Line 402: |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/8/8a/Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.txt">His+Dig+middellinker+FokA_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/7/71/Freiburg09_FluA_pMA.txt">FluA_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
Line 1,067: |
Line 408: |
| | | |
| | | |
- | </div><a name="14"></a>
| |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
- | </div>
| |
- | <div class="art-PostContent">
| |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| | | |
| | | |
- | <table border="5">
| |
- | <tr>
| |
- | <td align="center">
| |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/3/3b/Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.jpg" name="His+Dig+middellinker+Foki in pMA Vector" width="480" height="480" />
| |
- | <br><p align="center">His+Dig+middellinker+Foki in pMA Vector</p>
| |
- | </td>
| |
- | <td align="left">
| |
- | nucleotid sequence of ORF-1:<br>
| |
| | | |
- | ATGGCCGGC
| |
- | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| |
- | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| |
- | GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
| |
- | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
| |
- | CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
| |
- | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
| |
- | GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
| |
- | CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
| |
- | ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| |
- | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| |
- | ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
| |
- | ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
| |
- | TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
| |
- | CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
| |
- | GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
| |
- | ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
| |
- | AGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
| |
- | TGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCT
| |
- | GATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCT
| |
- | GAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCA
| |
- | AATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTG
| |
- | ACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGA
| |
- | GGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGG
| |
- | AAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
| |
| | | |
| | | |
- | </td>
| + | <a name="6"></a> |
- | </tr>
| + | <h3 class="art-PostHeaderIcon-wrapper"> DsbA_pMA<span class="art-PostHeader"></span> </h3> |
- | </table>
| + | |
- | <br>
| + | |
- | <h4>AA sequence of ORF-1:<br></h4>
| + | |
- | <table border="0">
| + | |
- | <p align="justify"><td align="left">
| + | |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
| + | |
- | SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
| + | |
- | YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| + | |
- | NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
| + | |
- | ILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQA
| + | |
- | DEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSN
| + | |
- | GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | <br><br></p></td></table>
| + | |
- | | + | |
- | <h4>Commented GenBank file</h4>
| + | |
- | <li> <a href="https://static.igem.org/mediawiki/2009/9/9f/Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.txt">His+Dig+middellinker+Foki_pMA.txt</a></li>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | </div><a name="15"></a>
| + | |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3> | + | |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
| | | |
- | <table border="5"> | + | <table border="5" style= "width: 100%;"> |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/5/58/Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.jpg" name="His+Dig+shortlinker+FokA in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/d/db/Freiburg09_DsbA_pMA.jpg/600px-Freiburg09_DsbA_pMA.jpg" name="DsbA in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">His+Dig+shortlinker+FokA in pMA Vector</p> | + | <br><p align="center">DsbA in pMA Vector</p> |
| + | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243002">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
| | | |
- | ATGGCCGGC
| + | ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC |
- | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| + | ATCGGCGACCGGTTA |
- | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| + | |
- | GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
| + | |
- | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
| + | |
- | CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
| + | |
- | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
| + | |
- | GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
| + | |
- | CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
| + | |
- | ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| + | |
- | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| + | |
- | ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
| + | |
- | ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC
| + | |
- | AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA
| + | |
- | TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC
| + | |
- | AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT
| + | |
- | GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCAT
| + | |
- | TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAG
| + | |
- | CCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAG
| + | |
- | CGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGA
| + | |
- | ATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCG
| + | |
- | TGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAAC
| + | |
- | CATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGAT
| + | |
- | TGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCC
| + | |
- | GTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| + | |
- | | + | |
- | | + | |
- | | + | |
| </td> | | </td> |
| </tr> | | </tr> |
Line 1,184: |
Line 438: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
| + | MKKIWLALAGLVLAFSASATG |
- | SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
| + | |
- | YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| + | |
- | NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM
| + | |
- | KVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQ
| + | |
- | RYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVL
| + | |
- | SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
| <br><br></p></td></table> | | <br><br></p></td></table> |
- |
| |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/d/d2/Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.txt">His+Dig+shortlinker+FokA_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/1/1b/Freiburg09_DsbA_pMA.txt">DsbA_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
| <br> | | <br> |
| | | |
- | </div><a name="16"></a>
| + | |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3> | + | <a name="7"></a> |
- | </div>
| + | <h3 class="art-PostHeaderIcon-wrapper"> StrepTag_pMA (iGEM team Freiburg 08)<span class="art-PostHeader"></span> </h3> |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
| | | |
- | <table border="5"> | + | <table border="5" style= "width: 100%;"> |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/1/15/Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.jpg/600px-Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.jpg" name="His+Dig+shortlinker+Foki in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/7/74/Freiburg09_StrepTag_pMA.jpg/600px-Freiburg09_StrepTag_pMA.jpg" name="StrepTag in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">His+Dig+shortlinker+Foki in pMA Vector</p> | + | <br><p align="center">StrepTag in pMA Vector</p> |
| + | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K157012">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
- | | + | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGTT |
- | ATGGCCGGC
| + | |
- | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| + | |
- | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| + | |
- | GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
| + | |
- | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
| + | |
- | CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
| + | |
- | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
| + | |
- | GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
| + | |
- | CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
| + | |
- | ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| + | |
- | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| + | |
- | ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
| + | |
- | ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC
| + | |
- | AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA
| + | |
- | TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC
| + | |
- | AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT
| + | |
- | GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCAT
| + | |
- | TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAG
| + | |
- | CGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAA
| + | |
- | CGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGA
| + | |
- | ATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCG
| + | |
- | TGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAAC
| + | |
- | CATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGAT
| + | |
- | CGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCC
| + | |
- | GTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
| + | |
- | | + | |
- | | + | |
| | | |
| | | |
Line 1,254: |
Line 472: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
| + | MAGWSHPQFEKTG |
- | SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
| + | |
- | YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| + | |
- | NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM
| + | |
- | KVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEME
| + | |
- | RYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVL
| + | |
- | SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| <br><br></p></td></table> | | <br><br></p></td></table> |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/b/b5/Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.txt">His+Dig+shortlinker+Foki_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/2/22/Freiburg09_StrepTag_pMA.txt">StrepTag_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
Line 1,274: |
Line 483: |
| | | |
| | | |
- | </div><a name="17"></a>
| + | <a name="8"></a> |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+Dig+Split+Foki_pMA<span class="art-PostHeader"></span> </h3> | + | <h3 class="art-PostHeaderIcon-wrapper"> HisTag_pMA (iGEM team Freiburg 08)<span class="art-PostHeader"></span> </h3> |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
| | | |
- | <table border="5"> | + | <table border="5" style= "width: 100%;"> |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/d/d7/Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.jpg" name="His+Dig+Split+Foki in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/8/8a/Freiburg09_HisTag_pMA.jpg/600px-Freiburg09_HisTag_pMA.jpg" name="HisTag in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">His+Dig+Split+Foki in pMA Vector</p><br> | + | <br><p align="center">HisTag in pMA Vector</p> |
- | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243018">link to part registry</a> | + | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K157011">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
- | | + | GCCGGCCATCATCATCATCATCATACCGGT |
- | ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
| + | |
- | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| + | |
- | ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
| + | |
- | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| + | |
- | GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
| + | |
- | CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
| + | |
- | ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
| + | |
- | CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
| + | |
- | GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| + | |
- | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| + | |
- | CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
| + | |
- | ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC
| + | |
- | TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA
| + | |
- | ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG
| + | |
- | TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA
| + | |
- | GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG
| + | |
- | CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTT
| + | |
- | ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCG
| + | |
- | TATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACG
| + | |
- | TTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAAT
| + | |
- | GGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTG
| + | |
- | AGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCA
| + | |
- | TATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCG
| + | |
- | GTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGT
| + | |
- | AAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
| + | |
- | | + | |
- | | + | |
| | | |
| | | |
Line 1,328: |
Line 509: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
| + | AGHHHHHHTG |
- | EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
| + | |
- | IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| + | |
- | CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE
| + | |
- | LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKA
| + | |
- | YSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA
| + | |
- | QLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| | | |
Line 1,343: |
Line 515: |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/1/11/Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.txt">His+Dig+Split+Foki_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/3/34/Freiburg09_HisTag_pMA.txt">HisTag_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
Line 1,349: |
Line 521: |
| | | |
| | | |
- | </div><a name="18"></a>
| + | <a name="9"></a> |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3> | + | <h3 class="art-PostHeaderIcon-wrapper"> shortlinker26_pMA <span class="art-PostHeader"></span> </h3> |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
| | | |
- | <table border="5"> | + | <table border="5" style= "width: 100%;"> |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/9/9f/Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.jpg" name="His+FluA+longlinker+FokA in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/7/7d/Freiburg09_Shortlinker26_pMA.jpg/600px-Freiburg09_Shortlinker26_pMA.jpg" name="shortlinker26 in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">His+FluA+longlinker+FokA in pMA Vector</p> | + | <br><p align="center">shortlinker26 in pMA Vector</p> |
| + | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243004">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
- | | + | ATGGCCGGCGGTGGTTCTGGTACCGGT |
- | ATGGCCGGC
| + | |
- | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| + | |
- | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| + | |
- | GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
| + | |
- | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
| + | |
- | CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
| + | |
- | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
| + | |
- | TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
| + | |
- | CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
| + | |
- | ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| + | |
- | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| + | |
- | ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
| + | |
- | GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT
| + | |
- | GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC
| + | |
- | TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC
| + | |
- | GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT
| + | |
- | CGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATAC
| + | |
- | CGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATA
| + | |
- | GCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTAT
| + | |
- | GTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTG
| + | |
- | GAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCG
| + | |
- | GCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAA
| + | |
- | ACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGG
| + | |
- | TGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAAT
| + | |
- | TCAACAATGGGGAGATCAATTTTACCGGTTAA
| + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| | | |
Line 1,402: |
Line 546: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
| + | MAGGGSGTG |
- | SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
| + | |
- | YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| + | |
- | NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
| + | |
- | MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
| + | |
- | VKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSV
| + | |
- | EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| | | |
Line 1,418: |
Line 552: |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/b/be/Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.txt">His+FluA+longlinker+FokA_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/0/0b/Freiburg09_Shortlinker26_pMA.txt">shortlinker26_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
Line 1,424: |
Line 558: |
| | | |
| | | |
- | </div><a name="19"></a>
| + | <a name="10"></a> |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3> | + | <h3 class="art-PostHeaderIcon-wrapper"> Middellinker38_pMA <span class="art-PostHeader"></span> </h3> |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
- | | + | <table border="5" style= "width: 100%;"> |
- | <table border="5"> | + | |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/6/6c/Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.jpg" name="His+FluA+longlinker+Foki in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/f/f6/Freiburg09_Middellinker38_pMA.jpg/600px-Freiburg09_Middellinker38_pMA.jpg" name="Middellinker38 in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">His+FluA+longlinker+Foki in pMA Vector</p><br> | + | <br><p align="center">Middellinker38 in pMA Vector</p> |
- | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243016">link to part registry</a> | + | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243005">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
- | | + | ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT |
- | ATGGCCGGC
| + | |
- | CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
| + | |
- | CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
| + | |
- | GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
| + | |
- | GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
| + | |
- | CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
| + | |
- | TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
| + | |
- | TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
| + | |
- | CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
| + | |
- | ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
| + | |
- | ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
| + | |
- | ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
| + | |
- | GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT
| + | |
- | GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC
| + | |
- | TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC
| + | |
- | GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT
| + | |
- | CGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATAC
| + | |
- | CGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATT
| + | |
- | CTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTAT
| + | |
- | GTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTG
| + | |
- | GAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCG
| + | |
- | GCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATC
| + | |
- | ACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGG
| + | |
- | AGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAAT
| + | |
- | TCAACAATGGCGAGATCAATTTTACCGGTTAA
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| </td> | | </td> |
Line 1,479: |
Line 581: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
| + | MAGGGSGGGSGTG |
- | SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
| + | |
- | YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
| + | |
- | NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
| + | |
- | MEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERY
| + | |
- | VEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSV
| + | |
- | EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| <br><br></p></td></table> | | <br><br></p></td></table> |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/7/77/Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.txt">His+FluA+longlinker+Foki_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/d/df/Freiburg09_Middellinker38_pMA.txt">Middellinker38_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
Line 1,501: |
Line 594: |
| | | |
| | | |
- | | + | <a name="11"></a> |
- | </div><a name="20"></a>
| + | <h3 class="art-PostHeaderIcon-wrapper"> LongLinkerA50_pMA <span class="art-PostHeader"></span> </h3> |
- | <h3 class="art-PostHeaderIcon-wrapper"> His+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3> | + | |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
- | | + | <table border="5" style= "width: 100%;"> |
- | <table border="5"> | + | |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/e/ef/Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.jpg" name="His+FluA+Split+FokA in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/e/ec/Freiburg09_LongLinkerA50_pMA.jpg/600px-Freiburg09_LongLinkerA50_pMA.jpg" name="LongLinkerA50 in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">His+FluA+Split+FokA in pMA Vector</p> | + | <br><p align="center">LongLinkerA50 in pMA Vector</p> |
| + | <li> <a href="http://partsregistry.org/Part:BBa_K243006">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
- | | + | ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT |
- | ATGGCC
| + | |
- | GGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTG
| + | |
- | CCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCA
| + | |
- | AGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAG
| + | |
- | TGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAG
| + | |
- | ATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACC
| + | |
- | CCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
| + | |
- | GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAA
| + | |
- | CTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACT
| + | |
- | GGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCC
| + | |
- | AAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCA
| + | |
- | GAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACA
| + | |
- | CCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATG
| + | |
- | AATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
| + | |
- | CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
| + | |
- | GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
| + | |
- | ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
| + | |
- | TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
| + | |
- | TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT
| + | |
- | GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT
| + | |
- | CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA
| + | |
- | AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG
| + | |
- | ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA
| + | |
- | AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG
| + | |
- | AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| + | |
- | | + | |
| | | |
| </td> | | </td> |
Line 1,552: |
Line 617: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEG
| + | MAGGGSGGGSGTG |
- | KSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYII
| + | |
- | GYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACK
| + | |
- | VNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
| + | |
- | ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA
| + | |
- | DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN
| + | |
- | GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
| | | |
| <br><br></p></td></table> | | <br><br></p></td></table> |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/5/53/Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.txt">His+FluA+Split+FokA_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/d/d2/Freiburg09_LongLinkerA50_pMA.txt">LongLinkerA50_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
Line 1,571: |
Line 629: |
| | | |
| | | |
- | </div><a name="21"></a>
| + | <a name="12"></a> |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3> | + | <h3 class="art-PostHeaderIcon-wrapper"> GSAT_linker_pMA <span class="art-PostHeader"></span> </h3> |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
- | | + | <table border="5" style= "width: 100%;"> |
- | <table border="5"> | + | |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/8/8b/Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.jpg" name="Strep+Dig+longlinker+FokA in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/1/11/Freiburg09_GSAT_linker_pMA.jpg/600px-Freiburg09_GSAT_linker_pMA.jpg" name="GSAT_linker in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">Strep+Dig+longlinker+FokA in pMA Vector</p> | + | <br><p align="center">GSAT_linker in pMA Vector</p> |
| + | <li> <a href="http://partsregistry.org/Part:BBa_K243029">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
- | | + | GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGCCGGT |
- | ATGGCC
| + | GGCTCCGGTTCTGGCTCCAGCGGTGGCAGCTCTGGTGCGTCCGGCACGGG |
- | GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
| + | TACTGCGGGTGGCACTGGCAGCGGTTCCGGTACTGGCTCTGGCACCGGTA |
- | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| + | ATACTAGTAGCGGCCGCTGCAGGGTACC |
- | ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
| + | |
- | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| + | |
- | GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
| + | |
- | CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
| + | |
- | ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
| + | |
- | CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
| + | |
- | GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| + | |
- | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| + | |
- | CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
| + | |
- | ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT
| + | |
- | GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA
| + | |
- | GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA
| + | |
- | TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA
| + | |
- | ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA
| + | |
- | TCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCC
| + | |
- | CGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTAT
| + | |
- | AACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAA
| + | |
- | CCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATC
| + | |
- | CGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAA
| + | |
- | GGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAA
| + | |
- | TGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCA
| + | |
- | AAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGG
| + | |
- | GAGATCAATTTTACCGGTTAA
| + | |
- | | + | |
| | | |
| | | |
Line 1,623: |
Line 656: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
| + | ELEFAAASRWPAVVLPVAPVLAPAVAALVRPARVLRVALAAVPVLALAPVILVAAAAGY |
- | EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
| + | |
- | IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| + | |
- | CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA
| + | |
- | RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGY
| + | |
- | NLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL
| + | |
- | NHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
| | | |
| | | |
Line 1,636: |
Line 662: |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/2/22/Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.txt">Strep+Dig+longlinker+FokA_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/1/14/Freiburg09_GSAT_linker_pMA.txt">GSAT_linker_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
| <br> | | <br> |
| | | |
- | </div><a name="22"></a>
| |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
- | </div>
| |
- | <div class="art-PostContent">
| |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| | | |
- | | + | <a name="13"></a> |
- | <table border="5">
| + | <h3 class="art-PostHeaderIcon-wrapper"> SEG_Linker_pMA <span class="art-PostHeader"></span> </h3> |
- | <tr>
| + | |
- | <td align="center">
| + | |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/6/65/Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.jpg" name="Strep+Dig+longlinker+Foki in pMA Vector" width="480" height="480" />
| + | |
- | <br><p align="center">Strep+Dig+longlinker+Foki in pMA Vector <br>
| + | |
- | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243011">link to part registry</a></li>
| + | |
- | </p>
| + | |
- | </td>
| + | |
- | <td align="left">
| + | |
- | nucleotid sequence of ORF-1:<br>
| + | |
- | | + | |
- | ATGGCC
| + | |
- | GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
| + | |
- | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| + | |
- | ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
| + | |
- | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| + | |
- | GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
| + | |
- | CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
| + | |
- | ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
| + | |
- | CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
| + | |
- | GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| + | |
- | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| + | |
- | CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
| + | |
- | ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT
| + | |
- | GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA
| + | |
- | GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA
| + | |
- | TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA
| + | |
- | ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA
| + | |
- | TCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCC
| + | |
- | CGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTAT
| + | |
- | AATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAA
| + | |
- | TCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATC
| + | |
- | CGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAA
| + | |
- | GGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAA
| + | |
- | TGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCA
| + | |
- | AAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGC
| + | |
- | GAGATCAATTTTACCGGTTAA
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | </td>
| + | |
- | </tr>
| + | |
- | </table>
| + | |
- | <br>
| + | |
- | <h4>AA sequence of ORF-1:<br></h4>
| + | |
- | <table border="0">
| + | |
- | <p align="justify"><td align="left">
| + | |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
| + | |
- | EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
| + | |
- | IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| + | |
- | CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA
| + | |
- | RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGY
| + | |
- | NLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL
| + | |
- | NHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | <br><br></p></td></table>
| + | |
- | | + | |
- | <h4>Commented GenBank file</h4>
| + | |
- | <li> <a href="https://static.igem.org/mediawiki/2009/4/4c/Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.txt">Strep+Dig+longlinker+Foki_pMA.txt</a></li>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | | + | |
- | | + | |
- | </div><a name="23"></a>
| + | |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3> | + | |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
- | | + | <table border="5" style= "width: 100%;"> |
- | <table border="5"> | + | |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/9/94/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+Dig+middellinker+FokA in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/0/09/Freiburg09_SEG_Linker.jpg/600px-Freiburg09_SEG_Linker.jpg" name="SEG_Linker in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">Strep+Dig+middellinker+FokA in pMA Vector</p> | + | <br><p align="center">SEG_Linker in pMA Vector</p> |
| + | <li> <a href="http://partsregistry.org/Part:BBa_K243030">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
- | | + | GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGGCGGC |
- | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| + | GGTTCTGAAGGTGGCGGCTCCGAAGGCGGCGGCAGCGAGGGCGGTGGTAG |
- | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| + | CGAAGGTGGTGGCTCCGAGGGTGGCGGTTCCGGCGGCGGTAGCACCGGTA |
- | AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
| + | ATACTAGTAGCGGCCGCTGCAGGGTACC |
- | AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| + | |
- | GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
| + | |
- | GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| + | |
- | TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
| + | |
- | CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
| + | |
- | AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| + | |
- | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| + | |
- | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
| + | |
- | AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
| + | |
- | GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA
| + | |
- | ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC
| + | |
- | TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA
| + | |
- | GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG
| + | |
- | TGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCG
| + | |
- | ATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTG
| + | |
- | CCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGAC
| + | |
- | TCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA
| + | |
- | GCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAAC
| + | |
- | TATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGC
| + | |
- | CGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCG
| + | |
- | GGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATC
| + | |
- | AATTTTACCGGTTAA
| + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| | | |
Line 1,770: |
Line 695: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
| + | ELEFAAASRWPAVVLAAVLKVAAPKAAAARAVVAKVVAPRVAVPAAVAPVILVAAAAGY |
- | TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
| + | |
- | NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| + | |
- | AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN
| + | |
- | STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNL
| + | |
- | PIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH
| + | |
- | KTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| <br><br></p></td></table> | | <br><br></p></td></table> |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/9/9f/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.txt">Strep+Dig+middellinker+FokA_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/d/d7/Freiburg09_SEG_Linker.txt">SEG_Linker_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
Line 1,791: |
Line 706: |
| | | |
| | | |
- | | + | <a name="14"></a> |
- | </div><a name="24"></a>
| + | <h3 class="art-PostHeaderIcon-wrapper"> Split_Linker_pMA (iGEM team Freiburg 08)<span class="art-PostHeader"></span> </h3> |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3> | + | |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
- | | + | <table border="5" style= "width: 100%;"> |
- | <table border="5"> | + | |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/7/79/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.jpg" name="Strep+Dig+middellinker+Foki in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/1/13/Freiburg09_Split-Linker_pMA.jpg/600px-Freiburg09_Split-Linker_pMA.jpg" name="Split_Linker in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">Strep+Dig+middellinker+Foki in pMA Vector<br> | + | <br><p align="center">Split_Linker in pMA Vector</p> |
- | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243012">link to part registry</a> | + | <li> <a href="http://partsregistry.org/Part:BBa_K157009">link to part registry</a> |
- | </p>
| + | |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
- | | + | ATGGCCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGT |
- | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| + | TATGAATCACACCGGT |
- | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| + | |
- | AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
| + | |
- | AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| + | |
- | GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
| + | |
- | GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| + | |
- | TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
| + | |
- | CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
| + | |
- | AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| + | |
- | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| + | |
- | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
| + | |
- | AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
| + | |
- | GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA
| + | |
- | ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC
| + | |
- | TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA
| + | |
- | GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG
| + | |
- | TGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCG
| + | |
- | ATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTG
| + | |
- | CCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGAC
| + | |
- | CCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA
| + | |
- | GTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAAC
| + | |
- | TATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGC
| + | |
- | AGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAG
| + | |
- | GCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATC
| + | |
- | AATTTTACCGGTTAA
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| </td> | | </td> |
Line 1,847: |
Line 730: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
| + | MAGRPACKIPNDLKQKVMNHTG |
- | TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
| + | |
- | NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| + | |
- | AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN
| + | |
- | STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNL
| + | |
- | PIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH
| + | |
- | ITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
| | | |
| <br><br></p></td></table> | | <br><br></p></td></table> |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/7/73/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.txt">Strep+Dig+middellinker+Foki_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/2/21/Freiburg09_Split-Linker_pMA.txt">Split_Linker_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
Line 1,864: |
Line 741: |
| | | |
| | | |
- | | + | <a name="15"></a> |
- | </div><a name="25"></a>
| + | <h3 class="art-PostHeaderIcon-wrapper"> YFP(Venus)_pGA14 (iGEM team Freiburg 08) <span class="art-PostHeader"></span> </h3> |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3> | + | |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
- | | + | <table border="5" style= "width: 100%;"> |
- | <table border="5"> | + | |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/9/97/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg" name="Strep+FluA+Split+FokA in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/6/61/Freiburg09_PGA14_YFP%28Venus%29.jpg/600px-Freiburg09_PGA14_YFP%28Venus%29.jpg" name="YFP(Venus) in pGA14 Vector" width="480" height="480" /> |
- | <br><p align="center">Strep+FluA+Split+FokA in pMA Vector</p> | + | <br><p align="center">YFP(Venus) in pGA14 Vector</p> |
| + | <li> <a href="http://partsregistry.org/Part:BBa_K157007">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
- | | + | ATGGCCGGCATGAGCAAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGAT |
- | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| + | TCTGGTGGAACTGGATGGTGATGTGAACGGCCATAAATTTAGCGTGAGCG |
- | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| + | GCGAAGGCGAAGGTGATGCGACCTATGGCAAACTGACCCTGAAACTGATT |
- | AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
| + | TGCACCACCGGCAAACTGCCGGTTCCGTGGCCGACCCTGGTTACCACCCT |
- | AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| + | GGGCTATGGCCTGCAATGCTTTGCGCGTTATCCGGATCATATGAAACAGC |
- | GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
| + | ACGATTTCTTTAAAAGCGCCATGCCGGAAGGCTATGTGCAGGAACGCACC |
- | GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| + | ATCTTTTTTAAAGATGATGGCAACTATAAAACCCGTGCGGAAGTGAAATT |
- | CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
| + | TGAAGGCGATACCCTGGTGAACCGTATTGAACTGAAAGGCATCGATTTCA |
- | CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
| + | AAGAAGATGGCAACATTCTGGGCCATAAACTGGAATATAACTACAACAGC |
- | AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| + | CATAACGTGTATATCACCGCGGATAAACAGAAAAACGGCATCAAAGCGAA |
- | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| + | CTTTAAAATCCGCCACAACATTGAAGATGGCGGCGTGCAGCTGGCCGATC |
- | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
| + | ATTATCAGCAGAACACCCCGATTGGTGATGGCCCGGTGCTGCTGCCGGAT |
- | AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG
| + | AACCATTATCTGAGCTACCAGAGCAAACTGAGCAAAGATCCGAACGAAAA |
- | CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC
| + | ACGTGACCATATGGTGCTGCTGGAATTTGTGACCGCGGCCGGTATTACCC |
- | CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
| + | ATGGCATGGATGAACTGTATAAAACCGGTTAA |
- | TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
| + | |
- | ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
| + | |
- | TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
| + | |
- | ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
| + | |
- | ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
| + | |
- | TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
| + | |
- | TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
| + | |
- | AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
| + | |
- | TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
| + | |
- | CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
| + | |
- | TTTACCGGTTAA
| + | |
- | | + | |
- | | + | |
| | | |
| </td> | | </td> |
Line 1,916: |
Line 778: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
| + | MAGMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPW |
- | TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
| + | PTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD |
- | NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| + | TLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQ |
- | AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
| + | LADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELY |
- | TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
| + | KTG |
- | IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
| + | |
- | TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
| | | |
| | | |
Line 1,928: |
Line 788: |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/7/73/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.txt">Strep+Dig+middellinker+Foki_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/2/2e/Freiburg09_PGA14_YFP%28Venus%29.txt">YFP(Venus)_pGA14.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
Line 1,934: |
Line 794: |
| | | |
| | | |
- | | + | <a name="19"></a> |
- | </div><a name="26"></a>
| + | <h3 class="art-PostHeaderIcon-wrapper"> Fos_pMA (iGEM team Freiburg 08) <span class="art-PostHeader"></span> </h3> |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3> | + | |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
- | | + | <table border="5" style= "width: 100%;"> |
- | <table border="5"> | + | |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/9/97/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg" name="Strep+FluA+Split+FokA in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/c/ca/Freiburg09_Fos_pMA.jpg/600px-Freiburg09_Fos_pMA.jpg" name="Fos in pMA Vector" width="480" height="480" /> |
- | <br><p align="center">Strep+FluA+Split+FokA in pMA Vector</p> | + | <br><p align="center">Fos) in pMA Vector</p> |
| + | <li> <a href="http://partsregistry.org/Part:BBa_K243027">link to part registry</a> |
| </td> | | </td> |
| <td align="left"> | | <td align="left"> |
| nucleotid sequence of ORF-1:<br> | | nucleotid sequence of ORF-1:<br> |
- | | + | ATGGCCGGCCACCATCACCACCACCATGGATCCGGTGAAGAAAAACGCCG |
- | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| + | TATCCGTCGTGAGCGTAACAAAATGGCAGCAGCCAAATCCCGTAATCGCC |
- | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| + | GTCGTGAGCTGACAGACACACTGCAAGCCGAAACCGATCAACTGGAGGAC |
- | AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
| + | GAGAAAAGTGCTCTGCAAACTGAGATTGCCAATCTGCTGAAAGAGAAAGA |
- | AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| + | AAAACTGGAGTTCATCCTGGCAGCAACCGGTTAA |
- | GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
| + | |
- | GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| + | |
- | CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
| + | |
- | CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
| + | |
- | AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| + | |
- | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| + | |
- | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
| + | |
- | AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG
| + | |
- | CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC
| + | |
- | CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
| + | |
- | TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
| + | |
- | ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
| + | |
- | TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
| + | |
- | ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
| + | |
- | ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
| + | |
- | TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
| + | |
- | TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
| + | |
- | AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
| + | |
- | TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
| + | |
- | CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
| + | |
- | TTTACCGGTTAA
| + | |
| | | |
| | | |
Line 1,985: |
Line 822: |
| <table border="0"> | | <table border="0"> |
| <p align="justify"><td align="left"> | | <p align="justify"><td align="left"> |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
| + | MAGHHHHHHGSGEEKRRIRRERNKMAAAKSRNRRRELTDTLQAETDQLEDEKSALQTEIA |
- | TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
| + | NLLKEKEKLEFILAATG |
- | NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| + | |
- | AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
| + | |
- | TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
| + | |
- | IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
| + | |
- | TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| + | |
| | | |
| | | |
Line 1,998: |
Line 830: |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/6/6a/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.txt">Strep+FluA+Split+FokA_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/8/80/Freiburg09_Fos_pMA.txt">Fos_pMA.txt</a></li> |
| <br> | | <br> |
| <br> | | <br> |
Line 2,004: |
Line 836: |
| | | |
| | | |
- | </div><a name="27"></a>
| |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
- | </div>
| |
- | <div class="art-PostContent">
| |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
| | | |
| | | |
- | <table border="5">
| |
- | <tr>
| |
- | <td align="center">
| |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/f/fb/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.jpg" name="Strep+Dig+shortlinker+FokA in pMA Vector" width="480" height="480" />
| |
- | <br><p align="center">Strep+Dig+shortlinker+FokA in pMA Vector</p>
| |
- | </td>
| |
- | <td align="left">
| |
- | nucleotid sequence of ORF-1:<br>
| |
| | | |
- | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
- | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
- | AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
| |
- | AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
- | GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
| |
- | GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
- | TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
| |
- | CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
| |
- | AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
- | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
- | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
| |
- | AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
| |
- | GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG
| |
- | CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG
| |
- | CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC
| |
- | TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA
| |
- | ACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA
| |
- | TCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAG
| |
- | GCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACA
| |
- | CATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGT
| |
- | TCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAG
| |
- | CTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGT
| |
- | AGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACAC
| |
- | TGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGT
| |
- | TAA
| |
| | | |
| | | |
- | </td>
| |
- | </tr>
| |
- | </table>
| |
- | <br>
| |
- | <h4>AA sequence of ORF-1:<br></h4>
| |
- | <table border="0">
| |
- | <p align="justify"><td align="left">
| |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
| |
- | TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
| |
- | NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
- | AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD
| |
- | RILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQ
| |
- | ADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNS
| |
- | NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| | | |
- | | + | <a name="16"></a> |
- | <br><br></p></td></table>
| + | <h3 class="art-PostHeaderIcon-wrapper"> pJS418_Phagemid-dummy <span class="art-PostHeader"></span> </h3> |
- | | + | |
- | <h4>Commented GenBank file</h4>
| + | |
- | <li> <a href="https://static.igem.org/mediawiki/2009/4/45/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.txt">Strep+Dig+shortlinker+FokA_pMA.txt</a></li>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | | + | |
- | </div><a name="28"></a>
| + | |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3> | + | |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
- | | + | <table border="5" style= "width: 100%;"> |
- | <table border="5"> | + | |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/4/4a/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.jpg" name="Strep+Dig+shortlinker+Foki in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/8/84/Freiburg09_PJS418_dummy.jpg/600px-Freiburg09_PJS418_dummy.jpg" name="pJS418 Vector with strong SD sequence" width="480" height="480" /> |
- | <br><p align="center">Strep+Dig+shortlinker+Foki in pMA Vector</p> | + | <br><p align="center">pJS418 Vector with strong SD sequence</p> |
| + | <li> <a href="http://partsregistry.org/Part:BBa_K243034">link to part registry</a> |
| </td> | | </td> |
- | <td align="left"> | + | <td align="center"> |
- | nucleotid sequence of ORF-1:<br>
| + | |
- | | + | |
- | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| + | |
- | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| + | |
- | AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
| + | |
- | AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| + | |
- | GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
| + | |
- | GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| + | |
- | TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
| + | |
- | CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
| + | |
- | AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| + | |
- | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| + | |
- | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
| + | |
- | AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
| + | |
- | GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG
| + | |
- | CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG
| + | |
- | CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC
| + | |
- | TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA
| + | |
- | ACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA
| + | |
- | TCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAG
| + | |
- | GCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACA
| + | |
- | TCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGT
| + | |
- | TCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAG
| + | |
- | CTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGT
| + | |
- | TGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTC
| + | |
- | TGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGT
| + | |
- | TAA
| + | |
- | | + | |
- | | + | |
| | | |
| + | <h4>Commented GenBank file</h4> |
| + | <li> <a href="https://static.igem.org/mediawiki/2009/3/3a/Freiburg09_PJS418_dummy.txt">pJS418.txt</a></li> |
| </td> | | </td> |
| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
| |
- | <h4>AA sequence of ORF-1:<br></h4>
| |
- | <table border="0">
| |
- | <p align="justify"><td align="left">
| |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
| |
- | TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
| |
- | NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
- | AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD
| |
- | RILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQ
| |
- | ADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNS
| |
- | NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
- |
| |
- |
| |
- | <br><br></p></td></table>
| |
- |
| |
- | <h4>Commented GenBank file</h4>
| |
- | <li> <a href="https://static.igem.org/mediawiki/2009/0/0f/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.txt">Strep+Dig+shortlinker+Foki_pMA.txt</a></li>
| |
| <br> | | <br> |
| <br> | | <br> |
Line 2,141: |
Line 866: |
| | | |
| | | |
- | | + | <a name="17"></a> |
- | </div><a name="29"></a>
| + | <h3 class="art-PostHeaderIcon-wrapper"> pJS419_Phagemid-dummy <span class="art-PostHeader"></span> </h3> |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3> | + | |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
- | | + | <table border="5" style= "width: 100%;"> |
- | <table border="5"> | + | |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/f/ff/Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.jpg" name="Strep+Dig+Split+FokA in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/b/b1/Freiburg09_PJS419_dummy.jpg/600px-Freiburg09_PJS419_dummy.jpg" name="pJS419 Vector with weak SD sequence" width="480" height="480" /> |
- | <br><p align="center">Strep+Dig+Split+FokA in pMA Vector</p><br> | + | <br><p align="center">pJS419 Vector with weak SD sequence</p> |
- | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243017">link to part registry</a> | + | <li> <a href="http://partsregistry.org/Part:BBa_K243035">link to part registry</a> |
| </td> | | </td> |
- | <td align="left"> | + | <td align="center"> |
- | nucleotid sequence of ORF-1:<br>
| + | |
- | | + | |
- | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| + | |
- | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| + | |
- | AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
| + | |
- | AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| + | |
- | GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
| + | |
- | GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| + | |
- | TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
| + | |
- | CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
| + | |
- | AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| + | |
- | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| + | |
- | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
| + | |
- | AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA
| + | |
- | CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC
| + | |
- | CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA
| + | |
- | AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT
| + | |
- | ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT
| + | |
- | CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTG
| + | |
- | CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACC
| + | |
- | AAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGAT
| + | |
- | GCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGA
| + | |
- | ACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTG
| + | |
- | TTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCT
| + | |
- | GAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGC
| + | |
- | TGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTT
| + | |
- | CGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| + | |
- | | + | |
- | | + | |
- | | + | |
| | | |
| + | <h4>Commented GenBank file</h4> |
| + | <li> <a href="https://static.igem.org/mediawiki/2009/d/d9/Freiburg09_PJS419_dummy.txt">pJS419.txt</a></li> |
| </td> | | </td> |
| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
| |
- | <h4>AA sequence of ORF-1:<br></h4>
| |
- | <table border="0">
| |
- | <p align="justify"><td align="left">
| |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
| |
- | TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
| |
- | NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
- | AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY
| |
- | IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDT
| |
- | KAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNY
| |
- | KAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| | | |
- |
| |
- |
| |
- | <br><br></p></td></table>
| |
- |
| |
- | <h4>Commented GenBank file</h4>
| |
- | <li> <a href="https://static.igem.org/mediawiki/2009/9/95/Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.txt">Strep+Dig+Split+FokA_pMA.txt</a></li>
| |
| <br> | | <br> |
| <br> | | <br> |
Line 2,215: |
Line 892: |
| | | |
| | | |
- | </div><a name="30"></a>
| + | <a name="18"></a> |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+Dig+Split+Foki_pMA<span class="art-PostHeader"></span> </h3> | + | <h3 class="art-PostHeaderIcon-wrapper"> pBAD_dummy <span class="art-PostHeader"></span> </h3> |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
- | | + | <table border="5" style= "width: 100%;"> |
- | <table border="5"> | + | |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/f/fe/Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.jpg" name="Strep+Dig+Split+Foki in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/6/69/Freiburg09_PBAD_dummy.jpg/600px-Freiburg09_PBAD_dummy.jpg" name="pBAD Vector" width="480" height="480" /> |
- | <br><p align="center">Strep+Dig+Split+Foki in pMA Vector</p><br> | + | <br><p align="center">pBAD Vector</p> |
- | <li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243015">link to part registry</a> | + | <li> <a href="http://partsregistry.org/Part:BBa_K243037">link to part registry</a> |
| </td> | | </td> |
- | <td align="left">
| |
- | nucleotid sequence of ORF-1:<br>
| |
- |
| |
- | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
- | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
- | AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
| |
- | AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
- | GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
| |
- | GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
- | TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
| |
- | CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
| |
- | AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
- | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
- | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
| |
- | AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA
| |
- | CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC
| |
- | CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA
| |
- | AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT
| |
- | ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT
| |
- | CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTG
| |
- | CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACA
| |
- | AAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGAT
| |
- | GGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGA
| |
- | ACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTG
| |
- | TTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCT
| |
- | GAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGC
| |
- | TGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTT
| |
- | CGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
| |
- |
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | </td>
| |
- | </tr>
| |
- | </table>
| |
- | <br>
| |
- | <h4>AA sequence of ORF-1:<br></h4>
| |
- | <table border="0">
| |
- | <p align="justify"><td align="left">
| |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
| |
- | TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
| |
- | NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
- | AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY
| |
- | IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVAT
| |
- | KAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNY
| |
- | KAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
- |
| |
- |
| |
- |
| |
- |
| |
- | <br><br></p></td></table>
| |
- |
| |
- | <h4>Commented GenBank file</h4>
| |
- | <li> <a href="https://static.igem.org/mediawiki/2009/6/63/Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.txt">Strep+Dig+Split+Foki_pMA.txt</a></li>
| |
- | <br>
| |
- | <br>
| |
- | <br>
| |
- |
| |
- |
| |
- | </div><a name="31"></a>
| |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| |
- | </div>
| |
- | <div class="art-PostContent">
| |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
- |
| |
- |
| |
- | <table border="5">
| |
- | <tr>
| |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/2/22/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.jpg" name="Strep+FluA+longlinker+FokA in pMA Vector" width="480" height="480" />
| |
- | <br><p align="center">Strep+FluA+longlinker+FokA in pMA Vector</p>
| |
- | </td>
| |
- | <td align="left">
| |
- | nucleotid sequence of ORF-1:<br>
| |
- |
| |
- | GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
| |
- | CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
| |
- | ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
| |
- | GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
| |
- | GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
| |
- | CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
| |
- | GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
| |
- | CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
| |
- | GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
| |
- | GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
| |
- | CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
| |
- | ACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACC
| |
- | GGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAA
| |
- | ATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTA
| |
- | CCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTC
| |
- | TATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGC
| |
- | CATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCA
| |
- | AAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATG
| |
- | CAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAA
| |
- | CGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGT
| |
- | TCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTG
| |
- | AACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCT
| |
- | GATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTC
| |
- | GCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| |
- | </td>
| |
- | </tr>
| |
- | </table>
| |
- | <br>
| |
- | <h4>AA sequence of ORF-1:<br></h4>
| |
- | <table border="0">
| |
- | <p align="justify"><td align="left">
| |
- | GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
| |
- | EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
| |
- | IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
| |
- | CKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILE
| |
- | MKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEM
| |
- | QRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAV
| |
- | LSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
- |
| |
- |
| |
- |
| |
- | <br><br></p></td></table>
| |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/d/d4/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.txt">Strep+FluA+longlinker+FokA_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/1/1c/Freiburg09_PBAD_dummy.txt">pBAD_dummy.txt</a></li> |
- | <br>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | | + | |
- | </div><a name="32"></a>
| + | |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| + | |
- | </div>
| + | |
- | <div class="art-PostContent">
| + | |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| + | |
- | | + | |
- | | + | |
- | <table border="5">
| + | |
- | <tr>
| + | |
- | <td align="center">
| + | |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/4/47/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.jpg" name="Strep+FluA+longlinker+Foki in pMA Vector" width="480" height="480" />
| + | |
- | <br><p align="center">Strep+FluA+longlinker+Foki in pMA Vector</p>
| + | |
- | </td>
| + | |
- | <td align="left">
| + | |
- | nucleotid sequence of ORF-1:<br>
| + | |
- | | + | |
- | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| + | |
- | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| + | |
- | AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
| + | |
- | AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| + | |
- | GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
| + | |
- | GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| + | |
- | CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
| + | |
- | CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
| + | |
- | AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| + | |
- | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| + | |
- | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
| + | |
- | AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCT
| + | |
- | GGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA
| + | |
- | ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA
| + | |
- | ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG
| + | |
- | AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC
| + | |
- | AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG
| + | |
- | CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT
| + | |
- | GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA
| + | |
- | CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT
| + | |
- | TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT
| + | |
- | CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA
| + | |
- | ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG
| + | |
- | AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
| + | |
- | | + | |
- | | + | |
| </td> | | </td> |
| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
| |
- | <h4>AA sequence of ORF-1:<br></h4>
| |
- | <table border="0">
| |
- | <p align="justify"><td align="left">
| |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
| |
- | TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
| |
- | NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
- | AACKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRI
| |
- | LEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQAD
| |
- | EMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNG
| |
- | AVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| | | |
- | <br><br></p></td></table>
| |
- |
| |
- | <h4>Commented GenBank file</h4>
| |
- | <li> <a href="https://static.igem.org/mediawiki/2009/c/ce/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.txt">Strep+FluA+longlinker+Foki_pMA.txt</a></li>
| |
| <br> | | <br> |
| <br> | | <br> |
Line 2,421: |
Line 917: |
| | | |
| | | |
- | </div><a name="33"></a>
| + | <a name="20"></a> |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3> | + | <h3 class="art-PostHeaderIcon-wrapper"> pEX_CatInsert <span class="art-PostHeader"></span> </h3> |
- | </div>
| + | |
| <div class="art-PostContent"> | | <div class="art-PostContent"> |
| <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> | | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font> |
| | | |
- | | + | <table border="5" style= "width: 100%;"> |
- | <table border="5"> | + | |
| <tr> | | <tr> |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/d/d8/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+FluA+middellinker+FokA in pMA Vector" width="480" height="480" /> | + | <img src="https://static.igem.org/mediawiki/2009/thumb/a/a6/Freiburg09_PEX_CATInsert.jpg/600px-Freiburg09_PEX_CATInsert.jpg" name="pEX Vector" width="480" height="480" /> |
- | <br><p align="center">Strep+FluA+middellinker+FokA in pMA Vector</p> | + | <br><p align="center">pEX Vector</p> |
| + | <li> <a href="http://partsregistry.org/Part:BBa_K243033">link to part registry</a> |
| </td> | | </td> |
- | <td align="left">
| |
- | nucleotid sequence of ORF-1:<br>
| |
- |
| |
- | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
- | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
- | AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
| |
- | AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
- | GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
| |
- | GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
- | CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
| |
- | CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
| |
- | AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
- | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
- | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
| |
- | AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
| |
- | TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
| |
- | GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
| |
- | ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
| |
- | TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTA
| |
- | TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCT
| |
- | ATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGT
| |
- | TATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATG
| |
- | GTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGA
| |
- | GCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCAT
| |
- | AAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGG
| |
- | CGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTA
| |
- | AATTCAACAATGGGGAGATCAATTTTACCGGTTAA
| |
- |
| |
- |
| |
- | </td>
| |
- | </tr>
| |
- | </table>
| |
- | <br>
| |
- | <h4>AA sequence of ORF-1:<br></h4>
| |
- | <table border="0">
| |
- | <p align="justify"><td align="left">
| |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
| |
- | TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
| |
- | NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
- | AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
| |
- | VMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
| |
- | YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLS
| |
- | VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
- |
| |
- |
| |
- | <br><br></p></td></table>
| |
- |
| |
- | <h4>Commented GenBank file</h4>
| |
- | <li> <a href="https://static.igem.org/mediawiki/2009/4/48/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.txt">Strep+FluA+middellinker+FokA_pMA.txt</a></li>
| |
- | <br>
| |
- | <br>
| |
- | <br>
| |
- |
| |
- |
| |
- | </div><a name="34"></a>
| |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
| |
- | </div>
| |
- | <div class="art-PostContent">
| |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| |
- |
| |
- |
| |
- | <table border="5">
| |
- | <tr>
| |
| <td align="center"> | | <td align="center"> |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/b/b9/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.jpg" name="Strep+FluA+middellinker+Foki in pMA Vector" width="480" height="480" />
| |
- | <br><p align="center">Strep+FluA+middellinker+Foki in pMA Vector</p>
| |
- | </td>
| |
- | <td align="left">
| |
- | nucleotid sequence of ORF-1:<br>
| |
- |
| |
- | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| |
- | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| |
- | AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
| |
- | AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| |
- | GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
| |
- | GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| |
- | CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
| |
- | CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
| |
- | AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| |
- | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| |
- | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
| |
- | AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
| |
- | TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
| |
- | GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
| |
- | ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
| |
- | TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTA
| |
- | TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGT
| |
- | ATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGT
| |
- | TATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATG
| |
- | GTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGA
| |
- | GCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCAT
| |
- | ATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGG
| |
- | TGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTA
| |
- | AATTCAACAATGGCGAGATCAATTTTACCGGTTAA
| |
- |
| |
- |
| |
- |
| |
- | </td>
| |
- | </tr>
| |
- | </table>
| |
- | <br>
| |
- | <h4>AA sequence of ORF-1:<br></h4>
| |
- | <table border="0">
| |
- | <p align="justify"><td align="left">
| |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
| |
- | TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
| |
- | NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
- | AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
| |
- | VMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMER
| |
- | YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLS
| |
- | VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
- |
| |
- |
| |
- |
| |
- | <br><br></p></td></table>
| |
| | | |
| <h4>Commented GenBank file</h4> | | <h4>Commented GenBank file</h4> |
- | <li> <a href="https://static.igem.org/mediawiki/2009/a/a3/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.txt">Strep+FluA+middellinker+Foki_pMA.txt</a></li> | + | <li> <a href="https://static.igem.org/mediawiki/2009/7/7a/Freiburg09_PEX_CATInsert.txt">pEX_CatInsert.txt</a></li> |
- | <br>
| + | |
- | <br>
| + | |
- | <br>
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | </div><a name="35"></a>
| + | |
- | <h3 class="art-PostHeaderIcon-wrapper"> Strep+FluA+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
| + | |
- | </div>
| + | |
- | <div class="art-PostContent">
| + | |
- | <div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
| + | |
- | | + | |
- | | + | |
- | <table border="5">
| + | |
- | <tr>
| + | |
- | <td align="center">
| + | |
- | <img src="https://static.igem.org/mediawiki/2009/thumb/d/db/Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.jpg" name="Strep+FluA+shortlinker+FokA in pMA Vector" width="480" height="480" />
| + | |
- | <br><p align="center">Strep+FluA+shortlinker+FokA in pMA Vector</p>
| + | |
- | </td>
| + | |
- | <td align="left">
| + | |
- | nucleotid sequence of ORF-1:<br>
| + | |
- | | + | |
- | ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
| + | |
- | CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
| + | |
- | AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
| + | |
- | AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
| + | |
- | GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
| + | |
- | GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
| + | |
- | CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
| + | |
- | CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
| + | |
- | AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
| + | |
- | ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
| + | |
- | GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
| + | |
- | AAGTGAACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAG
| + | |
- | GAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTA
| + | |
- | TATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGG
| + | |
- | AAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAA
| + | |
- | CATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTC
| + | |
- | CCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTT
| + | |
- | ATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAG
| + | |
- | AACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTA
| + | |
- | TCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTA
| + | |
- | AAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGC
| + | |
- | AATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGAT
| + | |
- | CAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATG
| + | |
- | GGGAGATCAATTTTACCGGTTAA
| + | |
- | | + | |
| </td> | | </td> |
| </tr> | | </tr> |
| </table> | | </table> |
- | <br>
| |
- | <h4>AA sequence of ORF-1:<br></h4>
| |
- | <table border="0">
| |
- | <p align="justify"><td align="left">
| |
- | MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
| |
- | TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
| |
- | NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
| |
- | AACKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEF
| |
- | FMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKE
| |
- | NQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEEL
| |
- | LIGGEMIKAGTLTLEEVRRKFNNGEINFTG
| |
| | | |
- |
| |
- | <br><br></p></td></table>
| |
- |
| |
- | <h4>Commented GenBank file</h4>
| |
- | <li> <a href="https://static.igem.org/mediawiki/2009/4/49/Freiburg09_Strep%2BFluA%2Bshortlinker%2BFokA_pMA.txt">Strep+FluA+shortlinker+FokA_pMA.txt</a></li>
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