Team:Freiburg bioware/cloning1

From 2009.igem.org

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  href="https://2009.igem.org/Team:Freiburg_bioware/Project#Summary">Summary</a>
  href="https://2009.igem.org/Team:Freiburg_bioware/Project#Summary">Summary</a>
       </li>
       </li>
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       <li><a
+
        
-
href="https://2009.igem.org/Team:Freiburg_bioware/Project#Subprojects">Subprojects</a></li>
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       <li><a
       <li><a
  href="https://2009.igem.org/Team:Freiburg_bioware/Project#Highlights">Highlights</a></li>
  href="https://2009.igem.org/Team:Freiburg_bioware/Project#Highlights">Highlights</a></li>
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  class="t">Human
  class="t">Human
Practice</span></a>
Practice</span></a>
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        <ul>
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      <li><a
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href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice/Ethics">Ethics</a>
 +
      </li>
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      <li><a
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href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice/Safety">Safety</a></li>
 +
     </ul>
 +
 
   </li>
   </li>
   <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Notebook"><span class="l"></span><span
   <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Notebook"><span class="l"></span><span
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  class="l"></span><span class="r"></span><span
  class="l"></span><span class="r"></span><span
  class="t">Collaboration</span></a></li>
  class="t">Collaboration</span></a></li>
 +
<li><a
 +
href="https://2009.igem.org/Team:Freiburg_bioware/Modeling"><span
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class="l"></span><span class="r"></span><span
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class="t">Modeling</span></a></li>
</ul>
</ul>
</div>
</div>
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</a><br>
</a><br>
*<a href="#15">YFP(Venus)_pGA14  
*<a href="#15">YFP(Venus)_pGA14  
 +
</a><br>
 +
*<a href="#19">Fos_pMA
</a><br>
</a><br>
*<a href="#16">pJS418 Vector
*<a href="#16">pJS418 Vector
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</a><br>
</a><br>
*<a href="#18">pBAD Vector
*<a href="#18">pBAD Vector
 +
</a><br>
 +
*<a href="#20">pEX Vector
</a><br>
</a><br>
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</td></tr></table>
</td></tr></table>
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<li style=" text-align:center;"><a href="https://2009.igem.org/Team:Freiburg_bioware/oligos">list of oligonucleotides</a></li>
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<br>
<br>
<br>
<br>
 +
 +
 +
<a name="19"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Fos_pMA (iGEM team Freiburg 08) <span class="art-PostHeader"></span> </h3>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 +
<table border="5" style= "width: 100%;">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/c/ca/Freiburg09_Fos_pMA.jpg/600px-Freiburg09_Fos_pMA.jpg" name="Fos in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Fos) in pMA Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243027">link to part registry</a>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
ATGGCCGGCCACCATCACCACCACCATGGATCCGGTGAAGAAAAACGCCG
 +
TATCCGTCGTGAGCGTAACAAAATGGCAGCAGCCAAATCCCGTAATCGCC
 +
GTCGTGAGCTGACAGACACACTGCAAGCCGAAACCGATCAACTGGAGGAC
 +
GAGAAAAGTGCTCTGCAAACTGAGATTGCCAATCTGCTGAAAGAGAAAGA
 +
AAAACTGGAGTTCATCCTGGCAGCAACCGGTTAA
 +
 +
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGHHHHHHGSGEEKRRIRRERNKMAAAKSRNRRRELTDTLQAETDQLEDEKSALQTEIA
 +
NLLKEKEKLEFILAATG
 +
 +
 +
 +
<br><br></p></td></table>
 +
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/8/80/Freiburg09_Fos_pMA.txt">Fos_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 +
 +
 +
 +
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<h4>Commented GenBank file</h4>
<h4>Commented GenBank file</h4>
<li> <a href="https://static.igem.org/mediawiki/2009/1/1c/Freiburg09_PBAD_dummy.txt">pBAD_dummy.txt</a></li>
<li> <a href="https://static.igem.org/mediawiki/2009/1/1c/Freiburg09_PBAD_dummy.txt">pBAD_dummy.txt</a></li>
 +
</td>
 +
</tr>
 +
</table>
 +
 +
<br>
 +
<br>
 +
<br>
 +
 +
 +
<a name="20"></a>
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;pEX_CatInsert <span class="art-PostHeader"></span> </h3>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 +
 +
<table border="5" style= "width: 100%;">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/a/a6/Freiburg09_PEX_CATInsert.jpg/600px-Freiburg09_PEX_CATInsert.jpg" name="pEX Vector" width="480" height="480" />
 +
<br><p align="center">pEX Vector</p>
 +
<li> <a href="http://partsregistry.org/Part:BBa_K243033">link to part registry</a>
 +
</td>
 +
<td align="center">
 +
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/7/7a/Freiburg09_PEX_CATInsert.txt">pEX_CatInsert.txt</a></li>
</td>
</td>
</tr>
</tr>

Latest revision as of 03:38, 22 October 2009

FREiGEM

 Index



 Parts

 FokA_pMA

back to index

FokA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGA TGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTG ATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGAT GAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAAT TCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACC CGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGA GCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAG AAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

    Commented GenBank file

  • FokA_pMA.txt



    •  Foki_pMA

    back to index

    Foki in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Foki_pMA.txt



    •  Dig_pMA

    back to index

    Dig in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGCAGGTGGCCG CTTATCCCGACCACATCACCAAGTACGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTACAGCGTGATCCACGG CAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCTACACCATCGGCGGCGTGACCCAGGTGGGC GTGAGCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACTT TTGCAGATACGACGAGGACAAGAAGGGCCACTGGGACGCCGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCAACTGGTCCCACCCCC AGTTCGAAAAGACCGGTTAA

    AA sequence of ORF-1:

    MAGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGKSVKVS RYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIGYFCRY DEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNSNW SHPQFEKTG

    Commented GenBank file

  • Dig_pMA.txt



    •  FluA_pMA

    back to index

    FluA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCA AGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGG CAAAGAATACTTCATGGAAGGCACCGCCTACCCCGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCCGGACCGTGGGCGGCTACACCAGAAAGACC GTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACAG CTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCGGTTAA

    AA sequence of ORF-1:

    MAGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGKSVKVS RYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIGYSCRY DEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNTG

    Commented GenBank file

  • FluA_pMA.txt



    •  DsbA_pMA

    back to index

    DsbA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGTTA

    AA sequence of ORF-1:

    MKKIWLALAGLVLAFSASATG

    Commented GenBank file

  • DsbA_pMA.txt



    •  StrepTag_pMA (iGEM team Freiburg 08)

    back to index

    StrepTag in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGTT

    AA sequence of ORF-1:

    MAGWSHPQFEKTG

    Commented GenBank file

  • StrepTag_pMA.txt



    •  HisTag_pMA (iGEM team Freiburg 08)

    back to index

    HisTag in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    GCCGGCCATCATCATCATCATCATACCGGT

    AA sequence of ORF-1:

    AGHHHHHHTG

    Commented GenBank file

  • HisTag_pMA.txt



    •  shortlinker26_pMA

    back to index

    shortlinker26 in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGTG

    Commented GenBank file

  • shortlinker26_pMA.txt



    •  Middellinker38_pMA

    back to index

    Middellinker38 in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGGGSGTG

    Commented GenBank file

  • Middellinker38_pMA.txt



    •  LongLinkerA50_pMA

    back to index

    LongLinkerA50 in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGGGSGTG

    Commented GenBank file

  • LongLinkerA50_pMA.txt



    •  GSAT_linker_pMA

    back to index

    GSAT_linker in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGCCGGT GGCTCCGGTTCTGGCTCCAGCGGTGGCAGCTCTGGTGCGTCCGGCACGGG TACTGCGGGTGGCACTGGCAGCGGTTCCGGTACTGGCTCTGGCACCGGTA ATACTAGTAGCGGCCGCTGCAGGGTACC

    AA sequence of ORF-1:

    ELEFAAASRWPAVVLPVAPVLAPAVAALVRPARVLRVALAAVPVLALAPVILVAAAAGY

    Commented GenBank file

  • GSAT_linker_pMA.txt



    •  SEG_Linker_pMA

    back to index

    SEG_Linker in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGGCGGC GGTTCTGAAGGTGGCGGCTCCGAAGGCGGCGGCAGCGAGGGCGGTGGTAG CGAAGGTGGTGGCTCCGAGGGTGGCGGTTCCGGCGGCGGTAGCACCGGTA ATACTAGTAGCGGCCGCTGCAGGGTACC

    AA sequence of ORF-1:

    ELEFAAASRWPAVVLAAVLKVAAPKAAAARAVVAKVVAPRVAVPAAVAPVILVAAAAGY

    Commented GenBank file

  • SEG_Linker_pMA.txt



    •  Split_Linker_pMA (iGEM team Freiburg 08)

    back to index

    Split_Linker in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGT TATGAATCACACCGGT

    AA sequence of ORF-1:

    MAGRPACKIPNDLKQKVMNHTG

    Commented GenBank file

  • Split_Linker_pMA.txt



    •  YFP(Venus)_pGA14 (iGEM team Freiburg 08)

    back to index

    YFP(Venus) in pGA14 Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCATGAGCAAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGAT TCTGGTGGAACTGGATGGTGATGTGAACGGCCATAAATTTAGCGTGAGCG GCGAAGGCGAAGGTGATGCGACCTATGGCAAACTGACCCTGAAACTGATT TGCACCACCGGCAAACTGCCGGTTCCGTGGCCGACCCTGGTTACCACCCT GGGCTATGGCCTGCAATGCTTTGCGCGTTATCCGGATCATATGAAACAGC ACGATTTCTTTAAAAGCGCCATGCCGGAAGGCTATGTGCAGGAACGCACC ATCTTTTTTAAAGATGATGGCAACTATAAAACCCGTGCGGAAGTGAAATT TGAAGGCGATACCCTGGTGAACCGTATTGAACTGAAAGGCATCGATTTCA AAGAAGATGGCAACATTCTGGGCCATAAACTGGAATATAACTACAACAGC CATAACGTGTATATCACCGCGGATAAACAGAAAAACGGCATCAAAGCGAA CTTTAAAATCCGCCACAACATTGAAGATGGCGGCGTGCAGCTGGCCGATC ATTATCAGCAGAACACCCCGATTGGTGATGGCCCGGTGCTGCTGCCGGAT AACCATTATCTGAGCTACCAGAGCAAACTGAGCAAAGATCCGAACGAAAA ACGTGACCATATGGTGCTGCTGGAATTTGTGACCGCGGCCGGTATTACCC ATGGCATGGATGAACTGTATAAAACCGGTTAA

    AA sequence of ORF-1:

    MAGMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPW PTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD TLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQ LADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELY KTG

    Commented GenBank file

  • YFP(Venus)_pGA14.txt



    •  Fos_pMA (iGEM team Freiburg 08)

    back to index

    Fos) in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCCACCATCACCACCACCATGGATCCGGTGAAGAAAAACGCCG TATCCGTCGTGAGCGTAACAAAATGGCAGCAGCCAAATCCCGTAATCGCC GTCGTGAGCTGACAGACACACTGCAAGCCGAAACCGATCAACTGGAGGAC GAGAAAAGTGCTCTGCAAACTGAGATTGCCAATCTGCTGAAAGAGAAAGA AAAACTGGAGTTCATCCTGGCAGCAACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHGSGEEKRRIRRERNKMAAAKSRNRRRELTDTLQAETDQLEDEKSALQTEIA NLLKEKEKLEFILAATG

    Commented GenBank file

  • Fos_pMA.txt



    •  pJS418_Phagemid-dummy

    back to index

    pJS418 Vector with strong SD sequence

  • link to part registry

    • Commented GenBank file

  • pJS418.txt
    		•



     pJS419_Phagemid-dummy

    back to index

    pJS419 Vector with weak SD sequence

  • link to part registry

    • Commented GenBank file

  • pJS419.txt
    		•



     pBAD_dummy

    back to index

    pBAD Vector

  • link to part registry

    • Commented GenBank file

  • pBAD_dummy.txt
    		•



     pEX_CatInsert

    back to index

    pEX Vector

  • link to part registry

    • Commented GenBank file

  • pEX_CatInsert.txt
    		•





    contact:  freigem09@googlemail.com

    Retrieved from "http://2009.igem.org/Team:Freiburg_bioware/cloning1"