Team:Paris

From 2009.igem.org

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== iGEM Paris Team ==
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==Message in a Bubble==
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Hello everyone! welcome to our wiki. It's the 3rd year for the Paris Team and we're still motivated to do our best. This year we have a team of 13 students (what a luck). We are 6 biologists, 2 mathematicians, 1 computer scientist, 1 sociologist, 1 infectiologist, 1 geneticist, and 1 chemist. We've got the best of the best of the best supervisors like Ariel Lindner, Guillaume Cambray and Samuel Bottani. So... let's the contest begin! (See our team [[Team:Paris/Team#top | here]]).
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==A Message in a Bubble==
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<center style="color:#b0310e; font-weight: bold; font-size: 2em;"> Interactive schema </center>
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===Abstract===
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'''Message in a Bubble: a robust inter-cellular communication system based on outer membrane vesicles.'''
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Sending a message across the ocean… Outer membrane vesicles (OMV), naturally produced by gram negative bacteria such as ''E. Coli'', are strong candidates for long-distance messaging. Our engineered communication platform consists in controlling OMV production by destabilizing membrane integrity through over-expression of specific periplasmic proteins (''e.g.'' TolR). In order to control and modulate message content, we used fusions with OmpA signal sequence and the ClyA hemolysin as delivery tags. A targeting system was developed, based on the outer-membrane expression of Jun/Fos leucine zippers to control the vesicle flux between donor and recipient cells. Once received, the signal from incoming vesicles is transduced through a modified Fec pathway, whereby the receptor is provided by the OMV. Computational models provided insight to all of the above steps. Such reliable communications systems have wide biotechnological implications, ranging from targeted drugs delivery and detoxification to advanced division of labor or even cell-based computing.
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===Strategy===
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We aimed developing a long distance communication system between gram-negative bacteria that is based on the ability of these organisms to produce Outer-Membrane Vesicles (OMVs). We designed a framework which can be easily expanded to a lot of different inputs/ouputs. We hope this standardized approach will increase our capacity to manipulate information and/or exchange it between bacteria. This can be used in any engineered biological process requiring transformation or a system of information transfer, including medical applications and bio-remediation.
We aimed developing a long distance communication system between gram-negative bacteria that is based on the ability of these organisms to produce Outer-Membrane Vesicles (OMVs). We designed a framework which can be easily expanded to a lot of different inputs/ouputs. We hope this standardized approach will increase our capacity to manipulate information and/or exchange it between bacteria. This can be used in any engineered biological process requiring transformation or a system of information transfer, including medical applications and bio-remediation.
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# the increase of vesicle production;  
# the increase of vesicle production;  
# the system to address proteins to these vesicles;
# the system to address proteins to these vesicles;
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# their fusion with target bacteria. You can find detailed information in our [[Team:Paris/Project#top | OMV Project]] description or in our different parts.
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# their fusion with target bacteria.  
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You can find detailed information in our [[Team:Paris/Project#top | OMV Project]] description or in our different parts.
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For more information on how we came up with this idea, please have a look at our [[Team:Paris/Brainstorm#top | brainstorming area]].  
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For more information on how we came up with this idea, please have a look at our [[Team:Paris/Brainstorm#top | brainstorming area]].
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===Navigation===
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<img src="https://static.igem.org/mediawiki/2009/b/ba/Paris01.png" height=500px>
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Here we are ! You have some problems to navigate in our wiki ?? Just look at [[Team:Paris/navigation#top | this page]], and we hope it will help you.  
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===Abstract===
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And, if your are interested in a guided visit of the iGEM Paris 2009 wiki,..., just open the book...
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'''Developing an accurate, reusable and versatile communication system between bacteria populations.'''
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Sending a message across the ocean….Outer membrane vesicles naturally produced by gram negative bacteria such as E. Coli are perfect candidates to send long-distance messages. They can be artificially produced by disturbing membrane integrity thanks to the overexpression of soluble parts of periplasmic proteins. Such vesicles contain periplasmic and outer-membrane proteins, thereby providing the opportunity to modulate their content through engineered targeting devices. To do so, we used the OmpA sequence signal and the ClyA hemolysin. An addressing system based on the jun/fos leucin zippers was developed to control the vesicle flux between donor and recipient cells. Once received, the signal from incoming vesicles is transduced down to the cytoplasm through a modified Fec pathway. The setup of a reliable communications systems such as the one we sought to develop has wide biotechnological implications, ranging from targeted drugs delivery and detoxification to advanced division of labor or even cell-based computing.
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== Our sponsors ==
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== Extended Team ==
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=== iGEM Paris Team ===
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'''Hello everyone and welcome on our wiki webpage !'''
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We are a team of 13 highly motivated students ready to give our best in the iGEM competition. We decided to involve our different backgrounds (6 biologists, 2 mathematicians, a computer scientist, a sociologist, an infectiologist, a geneticist, and a chemist) and our enthusiasm in this scientific and human adventure.
 +
Regarding the team's supervisors, we have the honnor to have been adviced by some great scientists/professors like Ariel Lindner, Guillaume Cambray and Samuel Bottani among others, almost always at the [http://www.cri-paris.org CRI].<br>
 +
 
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More details on our team [[Team:Paris/Team#top | here]].<br>
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More details on the CRI at the bottom of our [[Team:Paris/Acknowledgements#top | Acknowledgement page]].<br>
 +
Additional information about all iGEM Paris teams since 2007 here : [http://www.igem-paris.org http://www.igem-paris.org]<br>
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=== Our Sponsors ===
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<a href="http://www.ensmp.fr/">
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<a href="http://www.inria.fr/">
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== Visitors & Collaborations==
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=== Collaborations and  Visitors  ===
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With the [https://2009.igem.org/Team:BCCS-Bristol  team BCCS-Bristol] , we shared our modeling part concerning the production of vesicles which is supposed to be complementary of their and we hope that we will be able to combine both approaches to make one big model of the production, adressing, and fusion of the vesicles. For more details, see our [[Team:Paris/Collaborations#top | Collaborations]] web page.
 +
 
 +
 
 +
We have also created a poll concerning the Iphone Software and we sent it to the other iGEM teams, among which four replied. Find the results and more details on our [[Team:Paris/Collaborations#top | Collaborations]] page.
 +
 
 +
 
 +
All members of our team answered to the Valencia ethics poll that is why you can see this gorgeous gold medal with our name on it. More seriously it was very interesting to see what the members answered to each question. Looking for more details ? see our [[Team:Paris/Collaborations#top | Collaborations]] web page.
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And we thank all our visitors, without them we would not have such an eclectic cluster map !
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<a href="https://2009.igem.org/Team:Valencia/Human" target="_blank"> <img src="https://static.igem.org/mediawiki/2009/5/50/V_Paris.JPG" width="110" height="110"></a> Thank you <a href="https://2009.igem.org/Team:Valencia"><a3>Valencia</a3></a> !
<a href="https://2009.igem.org/Team:Valencia/Human" target="_blank"> <img src="https://static.igem.org/mediawiki/2009/5/50/V_Paris.JPG" width="110" height="110"></a> Thank you <a href="https://2009.igem.org/Team:Valencia"><a3>Valencia</a3></a> !
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== News ==
 
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* 28th August : new menu \o/ and 'breadcrumb'
 
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* 16th August : 1st Biobrick ! [http://partsregistry.org/Part:BBa_K257001  g3p - BBa K257001 ]
 
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* 30th July : Input Primers and Strain on the Wiki page [[Team:Paris/Freezer#top | here ]]
 
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* 23th July : First participation of the team in a popularization of science event in Paris.
 
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* 22th July : Let's start our Informatic tool for Labs manipulation on iPhone. see [[Team:Paris/Tool_introduction#top | here]].
 
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* 21th July : template site seems not to work well with IE and FF&Co, so we've got to change some BG but it's not essential right now
 
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* 20th July : Team image presentation is finish, now let's see what everyone gonna put in it.... Beginning of Labs work !! (finaly)
 
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* 15th July : NoteBook works, now we've got to fill it with some writting
 
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* 14th July : Hello ! The Wiki is under construction you may see some funny things, don't worry we'll be functional soon.
 

Latest revision as of 03:46, 22 October 2009

iGEM > Paris > Home > Synopsis

Contents

Message in a Bubble


Interactive schema

Abstract

Message in a Bubble: a robust inter-cellular communication system based on outer membrane vesicles. Sending a message across the ocean… Outer membrane vesicles (OMV), naturally produced by gram negative bacteria such as E. Coli, are strong candidates for long-distance messaging. Our engineered communication platform consists in controlling OMV production by destabilizing membrane integrity through over-expression of specific periplasmic proteins (e.g. TolR). In order to control and modulate message content, we used fusions with OmpA signal sequence and the ClyA hemolysin as delivery tags. A targeting system was developed, based on the outer-membrane expression of Jun/Fos leucine zippers to control the vesicle flux between donor and recipient cells. Once received, the signal from incoming vesicles is transduced through a modified Fec pathway, whereby the receptor is provided by the OMV. Computational models provided insight to all of the above steps. Such reliable communications systems have wide biotechnological implications, ranging from targeted drugs delivery and detoxification to advanced division of labor or even cell-based computing.

Strategy

We aimed developing a long distance communication system between gram-negative bacteria that is based on the ability of these organisms to produce Outer-Membrane Vesicles (OMVs). We designed a framework which can be easily expanded to a lot of different inputs/ouputs. We hope this standardized approach will increase our capacity to manipulate information and/or exchange it between bacteria. This can be used in any engineered biological process requiring transformation or a system of information transfer, including medical applications and bio-remediation.


We did our best to standardize:

  1. the increase of vesicle production;
  2. the system to address proteins to these vesicles;
  3. their fusion with target bacteria.

You can find detailed information in our OMV Project description or in our different parts.


By analogy to the Internet Protocol, we called our process a Bacterial Protocol. We found that the title Message in a bubble described it quite nicely. In a simple way, the comprehension of vesicles process sounds like a build of a bacteria language.


For more information on how we came up with this idea, please have a look at our brainstorming area.

Navigation

Here we are ! You have some problems to navigate in our wiki ?? Just look at this page, and we hope it will help you.

And, if your are interested in a guided visit of the iGEM Paris 2009 wiki,..., just open the book...

Open book.gif

← Previous - Next →

Extended Team

iGEM Paris Team

Hello everyone and welcome on our wiki webpage !


We are a team of 13 highly motivated students ready to give our best in the iGEM competition. We decided to involve our different backgrounds (6 biologists, 2 mathematicians, a computer scientist, a sociologist, an infectiologist, a geneticist, and a chemist) and our enthusiasm in this scientific and human adventure. Regarding the team's supervisors, we have the honnor to have been adviced by some great scientists/professors like Ariel Lindner, Guillaume Cambray and Samuel Bottani among others, almost always at the [http://www.cri-paris.org CRI].


More details on our team here.
More details on the CRI at the bottom of our Acknowledgement page.
Additional information about all iGEM Paris teams since 2007 here : [http://www.igem-paris.org http://www.igem-paris.org]


Our Sponsors

Collaborations and Visitors

With the team BCCS-Bristol , we shared our modeling part concerning the production of vesicles which is supposed to be complementary of their and we hope that we will be able to combine both approaches to make one big model of the production, adressing, and fusion of the vesicles. For more details, see our Collaborations web page.


We have also created a poll concerning the Iphone Software and we sent it to the other iGEM teams, among which four replied. Find the results and more details on our Collaborations page.


All members of our team answered to the Valencia ethics poll that is why you can see this gorgeous gold medal with our name on it. More seriously it was very interesting to see what the members answered to each question. Looking for more details ? see our Collaborations web page.


And we thank all our visitors, without them we would not have such an eclectic cluster map !


Locations of visitors to this page Thank you Valencia !

Retrieved from "http://2009.igem.org/Team:Paris"