Team:Tokyo-Nokogen/Project

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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:Tokyo-Nokogen | Team Example 2]]
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<!--- The Mission, Experiments --->
 
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<script type="text/JavaScript">
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!align="center"|[[Team:Tokyo-Nokogen|Home]]
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!align="center"|[[Team:Tokyo-Nokogen/Team|The Team]]
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!align="center"|[[Team:Tokyo-Nokogen/Project|The Project]]
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!align="center"|[[Team:Tokyo-Nokogen/Parts|Parts Submitted to the Registry]]
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}
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!align="center"|[[Team:Tokyo-Nokogen/Modeling|Modeling]]
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!align="center"|[[Team:Tokyo-Nokogen/Notebook|Notebook]]
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
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<td style="height:400px; background-color: #C00000;" ><img src="https://static.igem.org/mediawiki/2009/4/49/Mainpage5.png" width="750" align="middle" style="margin-left:15px"><br>
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Your abstract
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<td><a href="https://2009.igem.org/Team:Tokyo-Nokogen/Project" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('Image8','','https://static.igem.org/mediawiki/2009/f/f9/Project2.png',1)"><div align="center"><img src="https://static.igem.org/mediawiki/2009/9/9d/Project3.png" alt="" name="Image8" width="120" height="35" border="0"></div></td>
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<th scope="row"><a href="https://2009.igem.org/Team:Tokyo-Nokogen/Parts"
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<td><a href="https://2009.igem.org/Team:Tokyo-Nokogen/notebook" onMouseOut="MM_swapImgRestore()"onMouseOver="MM_swapImage('Image14','','https://static.igem.org/mediawiki/2009/b/bd/Notebook2.png',1)"><div align="center"><img src="https://static.igem.org/mediawiki/2009/0/0b/Notebook3.png" alt="" name="Image14" width="130" height="35" border="0"></div></td>
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<td><a href="https://2009.igem.org/Team:Tokyo-Nokogen/Safety" onMouseOut="MM_swapImgRestore()"onMouseOver="MM_swapImage('Image14','','https://static.igem.org/mediawiki/2009/b/bd/Notebook2.png',1)"><div align="center"><img src="https://static.igem.org/mediawiki/2009/e/eb/Safety3.png" alt="" name="Image14" width="110" height="35" border="0"></div></td>
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== Project Details==
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<img src="https://static.igem.org/mediawiki/2009/5/59/Overview.png" style="margin-left:45px"><div style="margin-left:45px; margin-right:15px">
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<h3><p style="margin-left:50px; margin-right:50px"> The production of recombinant protein is an important step to many experiments. Unfortunately, procedures can often be quite laborious and annoying (e.g., centrifugation, sonication,…). Our goal is to use synthetic biology to provide an easy solution. We named our system the <I>Escherichia coli</I> auto protein synthesizer (ESCAPES). It takes advantage of four concepts: 1) light switches, 2) cellular self-aggregation, 3) autolysis, and 4) transcriptional signal counter. Click on the labels in the figure below for further details. With our ESCAPES system, we would only need to irradiate the cells twice to produce a crude extract of our target protein.
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<table width="730" height="600" border="0" style="background:url(https://static.igem.org/mediawiki/2009/4/48/ESCAPeS1.png) no-repeat; margin-left:50px">
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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<a href="https://2009.igem.org/Team:Tokyo-Nokogen/Project/Lysis"><p style="text-indent: 33em"><img src="https://static.igem.org/mediawiki/2009/f/fb/Paper2.png" width="160" height="74" border="0">
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<br>
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<a href="https://2009.igem.org/Team:Tokyo-Nokogen/Project/Aggregation"><p style="text-indent: 22em"><img src="https://static.igem.org/mediawiki/2009/f/fb/Paper2.png" width="170" height="74" border="0">
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<a href="https://2009.igem.org/Team:Tokyo-Nokogen/Project/Light-receptor"><p style="text-indent: 5em"><img src="https://static.igem.org/mediawiki/2009/f/fb/Paper2.png" width="240" height="74" border="0"></a>
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<a href="https://2009.igem.org/Team:Tokyo-Nokogen/Project/Counter"><img src="https://static.igem.org/mediawiki/2009/f/fb/Paper2.png" width="180" height="74" border="0" style="margin-left:200px; margin-bottom:0px"></td></a>
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</table>
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=== Part 2 ===
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<p style="margin-left:50px; margin-right:50px"> The first induction with green light induces the expression of antigen 43, which causes the <I>E.coli</I> to self-aggregate and settle to the bottom. The medium is then decanted and replaced with a small volume of buffer. The aggregated cells are once again irradiated with green light, inducing the expression of holin and endolysin, which causes the autolysis of the bacterial cells. ESCAPES will allow us to escape from the annoying centrifugation and lysis steps, to conveniently produce crude extracts of our target protein.
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<p align="center"><a href="https://2009.igem.org/Team:Tokyo-Nokogen/Project">
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                                  <img src="https://static.igem.org/mediawiki/2009/9/92/TOP2.png" width="63" height="50" border="0"></a></p>
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=== The Experiments ===
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=== Part 3 ===
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<td  style="height:80px">
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== Results ==
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<div class=""footer>   <br style="line-height:9px"><div align="center">
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<strong class="footer">Copyright 2009 &copy; Tokyo-Nokogen. All rights reserved.</strong><br>
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Latest revision as of 03:57, 22 October 2009




The production of recombinant protein is an important step to many experiments. Unfortunately, procedures can often be quite laborious and annoying (e.g., centrifugation, sonication,…). Our goal is to use synthetic biology to provide an easy solution. We named our system the Escherichia coli auto protein synthesizer (ESCAPES). It takes advantage of four concepts: 1) light switches, 2) cellular self-aggregation, 3) autolysis, and 4) transcriptional signal counter. Click on the labels in the figure below for further details. With our ESCAPES system, we would only need to irradiate the cells twice to produce a crude extract of our target protein.



















The first induction with green light induces the expression of antigen 43, which causes the E.coli to self-aggregate and settle to the bottom. The medium is then decanted and replaced with a small volume of buffer. The aggregated cells are once again irradiated with green light, inducing the expression of holin and endolysin, which causes the autolysis of the bacterial cells. ESCAPES will allow us to escape from the annoying centrifugation and lysis steps, to conveniently produce crude extracts of our target protein.



Copyright 2009 © Tokyo-Nokogen. All rights reserved.

Retrieved from "http://2009.igem.org/Team:Tokyo-Nokogen/Project"