Team:Utah State/Parts

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         <td width="172" id="ana"><span class="currentPage"><font size = 4>HOME</font></span><a href="#LaLa">Introduction</a><br />
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         <td id="nav"><a href="https://2009.igem.org/Team:Utah_State"><font size = 4>HOME</font></a></td>
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         <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Project"><font size = 4>PROJECT</font></a></td>
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    <td width="172" id="ana"><span class="currentPage"><font size = 4>BIOBRICKS</font></span>
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      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Modeling"><font size = 4>MODELING</font></a></td>
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        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Parts"><font size = 4>PARTS</font></a></td>
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      <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/ETHICS"><font size = 4>ETHICS</font></a></td>
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               <b><i>BioBricks without Borders:</b></i></font>
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               <b><i>BioBricks</b></i></font>
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              <p><font face="Helvetica, Arial, San Serif" color =green>Investigating a multi-host BioBrick vector and secretion of cellular products</font></p><HR>
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<p class="class">The USU iGEM team has successfully constructed 49 BioBrick parts and devices. Of these, we have had time to demonstrate the functionality of 8 BioBricks, and had 41 of them sequenced. Check out our <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Utah_State"><font color=#009900>BioBrick Registry</font></b></a> page to see all of these parts and to find more detailed information about them. The following figures shows all of the potential different combinations of pieces used to make composite devices for this project.
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<p> <font size="2.5" face=" Tahoma, Helvetica, Arial" color =#000000>The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like <i>Pseudomonas putida</i>, <i>Rhodobacter sphaeroides</i>, and <i>Synechocystis</i> PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation.  Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions.  To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.</p>
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            <p> <font size="4" face="Helvetica, Arial, San Serif" color =green>OUR SITE IS STILL UNDER CONSTRUCTION AND OUR INFORMATION IS BEING ADDED.  PLEASE COME BACK IN A FEW WEEKS TO SEE OUR PROJECT!</font></p>
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<div align="center"> <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Utah_State"><img src="https://static.igem.org/mediawiki/2009/c/ca/Slide5USU.jpg " align = "middle" height="350" style="float:left; alt="SBC"> </a> </div>
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Latest revision as of 22:11, 20 November 2009

USU iGem Untitled Document

BIOBRICKS
BioBricks

The USU iGEM team has successfully constructed 49 BioBrick parts and devices. Of these, we have had time to demonstrate the functionality of 8 BioBricks, and had 41 of them sequenced. Check out our BioBrick Registry page to see all of these parts and to find more detailed information about them. The following figures shows all of the potential different combinations of pieces used to make composite devices for this project.



Retrieved from "http://2009.igem.org/Team:Utah_State/Parts"