|
|
| (5 intermediate revisions not shown) |
| Line 2: |
Line 2: |
| | | | |
| | | | |
| - | <div title="Arsie Says UP TO ACCUMULATION" style="float:right" >{{linkedImage|Next.JPG|Team:Groningen/Parts/Submitted_Parts}}</div> | + | <div style="float:left" >{{linkedImage|GroningenPrevious.png|Team:Groningen/Ethics/Survey}}</div> |
| | + | <div title="Arsie Says UP TO ACCUMULATION" style="float:right">{{linkedImage|Next.JPG|Team:Groningen/Parts/Submitted_Parts}}</div> |
| | | | |
| | | | |
| - | ==Parts==
| |
| | | | |
| - | {{todo}}Here we need to give some genereal information about our parts.
| |
| | | | |
| | *For a comprehensive list of all the parts we used, have a look at our [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen '''parts registry''']. | | *For a comprehensive list of all the parts we used, have a look at our [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen '''parts registry''']. |
| | *Here you can find an informative overview of our [[Team:Groningen/Modelling/Submitted Parts|'''submitted parts''']]. | | *Here you can find an informative overview of our [[Team:Groningen/Modelling/Submitted Parts|'''submitted parts''']]. |
| | *Experience we had with parts from the registry can be found under [[Team:Groningen/Modelling/Used Parts|'''used parts''']]. | | *Experience we had with parts from the registry can be found under [[Team:Groningen/Modelling/Used Parts|'''used parts''']]. |
| | + | *Need anything specific? Take a look in our [[Team:Groningen/Parts/Freezer|freezer]] |
| | | | |
| | | | |
| - | <!--- The Mission, Experiments --->
| |
| - |
| |
| - | ===Note===
| |
| - |
| |
| - | If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
| |
| - |
| |
| - | :::::::::::::::http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
| |
| - |
| |
| - | <b><h2>[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]</h2></b>
| |
| - |
| |
| - |
| |
| - | ===Part List -80===
| |
| - |
| |
| - | {|cellpadding="10" cellspacing="1" border="10" style="font-size: small;"
| |
| - | |'''Component'''
| |
| - | |'''Description'''
| |
| - | |'''Part or Accession #'''
| |
| - | |'''Base Pairs (bp)'''
| |
| - | |'''Plasmid (backbone)'''
| |
| - | |'''Resistance'''
| |
| - | |'''Well'''
| |
| - | |'''Quality control'''
| |
| - | |-
| |
| - | |GVP
| |
| - | |Gas Vesicle Proteins
| |
| - | |<partinfo>BBa_I750016</partinfo>
| |
| - | |6064
| |
| - | |<partinfo>BBa_J61035</partinfo>
| |
| - | |Ampicillin/Gentamycin
| |
| - | |plate 3, pos.1
| |
| - | |?
| |
| - | |-
| |
| - | |GVP (2nd batch)
| |
| - | |Gas Vesicle Proteins
| |
| - | |<partinfo>BBa_I750016</partinfo>
| |
| - | |6064
| |
| - | |<partinfo>BBa_J61035</partinfo>
| |
| - | |Ampicillin/Gentamycin
| |
| - | |plate 3, pos.2
| |
| - | |?
| |
| - | |-
| |
| - | |pHigh in low copy nr vector
| |
| - | |High consitutive promoter
| |
| - | |<partinfo>J23100</partinfo>
| |
| - | |35
| |
| - | |<partinfo>pSB3K3</partinfo>
| |
| - | |Kanamycin
| |
| - | |Plate 3, pos. 4
| |
| - | |PCR(?), restriction(+)
| |
| - | |-
| |
| - | |pMed in low copy nr vector
| |
| - | |Medium consitutive promoter
| |
| - | |<partinfo>J23106</partinfo>
| |
| - | |35
| |
| - | |<partinfo>pSB3K3</partinfo>
| |
| - | |Kanamycin
| |
| - | |Plate 3, pos. 5
| |
| - | |PCR(?), restriction(+)
| |
| - | |-
| |
| - | |pLow in low copy nr vector
| |
| - | |Low consitutive promoter
| |
| - | |<partinfo>J23109</partinfo>
| |
| - | |35
| |
| - | |<partinfo>pSB3K3</partinfo>
| |
| - | |Kanamycin
| |
| - | |Plate 3, pos. 6
| |
| - | |PCR(?), restriction(+)
| |
| - | |-
| |
| - | |pHigh in high copy nr vector
| |
| - | |High consitutive promoter
| |
| - | |<partinfo>J23100</partinfo>
| |
| - | |35
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/Chloramphenicol
| |
| - | |Plate 3, pos. 7
| |
| - | |PCR(?), restriction(+)
| |
| - | |-
| |
| - | |pMed in high copy nr vector
| |
| - | |Medium consitutive promoter
| |
| - | |<partinfo>J23106</partinfo>
| |
| - | |35
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/Chloramphenicol
| |
| - | |Plate 3, pos. 8
| |
| - | |PCR(?), restriction(+)
| |
| - | |-
| |
| - | |pLow in high copy nr vector
| |
| - | |High consitutive promoter
| |
| - | |<partinfo>J23109</partinfo>
| |
| - | |35
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/Chloramphenicol
| |
| - | |Plate 3, pos. 9
| |
| - | |PCR(?), restriction(+)
| |
| - | |-
| |
| - | |Promotor (low expression)
| |
| - | |Constitutive promotor
| |
| - | |<partinfo>BBa_J23109</partinfo>
| |
| - | |35
| |
| - | |<partinfo>BBa_J61002</partinfo>
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.10
| |
| - | |No
| |
| - | |-
| |
| - | |Promotor (medium expression)
| |
| - | |Constitutive promotor
| |
| - | |<partinfo>BBa_J23106</partinfo>
| |
| - | |35
| |
| - | |<partinfo>BBa_J61002</partinfo>
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.11
| |
| - | |No
| |
| - | |-
| |
| - | |Promotor (high expression)
| |
| - | |Constitutive promotor
| |
| - | |<partinfo>BBa_J23100</partinfo>
| |
| - | |35
| |
| - | |<partinfo>BBa_J61002</partinfo>
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.12
| |
| - | |No
| |
| - | |-
| |
| - | |Inducible promoter
| |
| - | |Lactose inducible promoter nr1
| |
| - | |<partinfo>R0010</partinfo>
| |
| - | |200
| |
| - | |<partinfo>pSB1A2</partinfo>
| |
| - | |Ampicillin
| |
| - | |Plate 3, pos. 14
| |
| - | |PCR(?), restriction(+)
| |
| - | |-
| |
| - | |Inducible promoter
| |
| - | |Lactose inducible promoter nr2
| |
| - | |<partinfo>R0010</partinfo>
| |
| - | |200
| |
| - | |<partinfo>pSB1A2</partinfo>
| |
| - | |Ampicillin
| |
| - | |Plate 3, pos. 15
| |
| - | |PCR(?), restriction(+)
| |
| - | |-
| |
| - | |RBS
| |
| - | |Ribosomal Binding Site
| |
| - | |<partinfo>BBa_B0034</partinfo>
| |
| - | |12
| |
| - | |<partinfo>pSB1A2</partinfo>
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.19
| |
| - | |No
| |
| - | |-
| |
| - | |Terminator
| |
| - | |Double terminator consisting of <partinfo>BBa_B0012</partinfo> and <partinfo>BBa_B0011</partinfo>
| |
| - | |<partinfo>BBa_B0014</partinfo>
| |
| - | |~90
| |
| - | |<partinfo>pSB1AK3</partinfo>
| |
| - | |Ampicillin/Kanamycin
| |
| - | |plate 3, pos.20
| |
| - | |No
| |
| - | |-
| |
| - | |Const. Promoter + GVP
| |
| - | |Low constitutive promoter with GVP cluster
| |
| - | |<partinfo>BBa_J23109</partinfo> + <partinfo>BBa_I750016</partinfo>
| |
| - | |~6100
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/Chloramphenicol
| |
| - | |plate 3, pos.21
| |
| - | |<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
| |
| - | |-
| |
| - | |Const. Promoter + GVP (no.2)
| |
| - | |Low constitutive promoter with GVP cluster
| |
| - | |<partinfo>BBa_J23109</partinfo> + <partinfo>BBa_I750016</partinfo>
| |
| - | |~6100
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/Chloramphenicol
| |
| - | |plate 3, pos.22
| |
| - | |<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
| |
| - | |-
| |
| - | |Const. Promoter + GVP
| |
| - | |Medium constitutive promoter with GVP cluster
| |
| - | |<partinfo>BBa_J23106</partinfo> + <partinfo>BBa_I750016</partinfo>
| |
| - | |~6100
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/Chloramphenicol
| |
| - | |plate 3, pos.23
| |
| - | |<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
| |
| - | |-
| |
| - | |Const. Promoter + GVP (no.2)
| |
| - | |Medium constitutive promoter with GVP cluster
| |
| - | |<partinfo>BBa_J23106</partinfo> + <partinfo>BBa_I750016</partinfo>
| |
| - | |~6100
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/Chloramphenicol
| |
| - | |plate 3, pos.24
| |
| - | |<i>Eco</i>RI/<i>Pst</i>I + {{done|Sequenced Okay}}
| |
| - | |-
| |
| - | |HmtA (1.1)
| |
| - | |HmtA-His in pBAD(a)
| |
| - | |None (to become <partinfo>BBa_K190018</partinfo>)
| |
| - | |2004
| |
| - | |[http://ecoliwiki.net/colipedia/index.php/pBAD/myc-His_A pBAD]
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.28
| |
| - | |Restriction(+)
| |
| - | |-
| |
| - | |HmtA (2.1)
| |
| - | |HmtA-His in pBAD(a)
| |
| - | |None (to become <partinfo>BBa_K190018</partinfo>)
| |
| - | |2004
| |
| - | |[http://ecoliwiki.net/colipedia/index.php/pBAD/myc-His_A pBAD]
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.29
| |
| - | |Restriction(+)
| |
| - | |-
| |
| - | |RBS MBPArsR
| |
| - | | <partinfo>BBa_B0034</partinfo> + <partinfo>BBa_K190027</partinfo>
| |
| - | |None (to become <partinfo>BBa_K190018</partinfo>)
| |
| - | |~1600
| |
| - | |<partinfo>pSB1A2</partinfo>
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.30,31,32
| |
| - | |Restriction(+)PCR
| |
| - | |-
| |
| - | |pLac-fMT + Ars-RFP
| |
| - | |pArs testing
| |
| - | |<partinfo>BBa_K190032</partinfo> <partinfo>BBa_K190023</partinfo> <partinfo>BBa_J61002</partinfo> (without the backbone)
| |
| - | |~1400
| |
| - | |<partinfo>pSB1A2</partinfo>
| |
| - | |Ampicillin
| |
| - | |plate 3, pos. 33,34,35
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |CueO- + GVP in J61035
| |
| - | |CueO promoter without RBS in front of the GVP cluster
| |
| - | |None
| |
| - | |6149
| |
| - | |<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I)
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.37
| |
| - | |Restriction(+) + {{done|Sequenced Okay}}
| |
| - | |-
| |
| - | |ZntR- + GVP in J61035
| |
| - | |ZntR promoter without RBS in front of the GVP cluster
| |
| - | |None
| |
| - | |~6150
| |
| - | |<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I)
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.38
| |
| - | |Restriction(+) + {{done|Sequenced (3 mutations)}}
| |
| - | |-
| |
| - | |ArsR- + GVP in J61035
| |
| - | |ArsR promoter without RBS in front of the GVP cluster
| |
| - | |None
| |
| - | |~6150
| |
| - | |<partinfo>BBa_J61035</partinfo> (without the part between <i>Eco</i>RI and <i>Xba</i>I)
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.39
| |
| - | |Restriction(+) + {{done|Sequenced Okay}}
| |
| - | |-
| |
| - | |ArsR- in J61002
| |
| - | |pArsR (promoter without RBS)
| |
| - | |<partinfo>BBa_K190015</partinfo>
| |
| - | |71
| |
| - | |<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.40
| |
| - | |<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
| |
| - | |-
| |
| - | |ZntR- in J61002
| |
| - | |pZntR (promoter without RBS)
| |
| - | |<partinfo>BBa_K190016</partinfo>
| |
| - | |65
| |
| - | |<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.41
| |
| - | |<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced (3 mutations)}}
| |
| - | |-
| |
| - | |CueO- in J61002
| |
| - | |pCueO (promoter without RBS)
| |
| - | |<partinfo>BBa_K190017</partinfo>
| |
| - | |43
| |
| - | |<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.42
| |
| - | |<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
| |
| - | |-
| |
| - | |ZntR+RBS in J61002
| |
| - | |pZntR (promoter with own RBS)
| |
| - | |<partinfo>BBa_K190022</partinfo>
| |
| - | |91
| |
| - | |<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.43
| |
| - | |<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
| |
| - | |-
| |
| - | |ArsR+RBS in J61002
| |
| - | |pArsR (promoter with own RBS)
| |
| - | |<partinfo>BBa_K190023</partinfo>
| |
| - | |81
| |
| - | |<partinfo>BBa_J61002</partinfo> (without the part between <i>Eco</i>RI and <i>Spe</i>I)
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.44
| |
| - | |<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
| |
| - | |-
| |
| - | |CueO+RBS in J61002
| |
| - | |pCueO (promoter with own RBS)
| |
| - | |<partinfo>BBa_K190024</partinfo>
| |
| - | |71
| |
| - | |<partinfo>BBa_J61002</partinfo> (without the part between EcoRI and SpeI)
| |
| - | |Ampicillin
| |
| - | |plate 3, pos.45
| |
| - | |<i>Eco</i>RI/<i>Spe</i>I(+), <i>Eco</i>RI/<i>Pst</i>I(+), {{done|Sequenced Okay}}
| |
| - | |-
| |
| - | |SmtA-GST in pGEX-3x
| |
| - | |SmtA-GST fusion
| |
| - | |no biobrick primers available
| |
| - | |1400
| |
| - | |[http://www.ecoliwiki.net/colipedia/index.php/pGEX-3X pGEX-3x]
| |
| - | |Ampicillin
| |
| - | |plate 3, pos. 46
| |
| - | |PCR, restriction
| |
| - | |-
| |
| - | |SmtA in pET29a
| |
| - | |SmtA
| |
| - | |<partinfo>BBa_K190021</partinfo>
| |
| - | |900
| |
| - | |[http://www.merckbiosciences.co.uk/product/69871 pET29a]
| |
| - | |Kanamycin
| |
| - | |plate 3, pos. 47
| |
| - | |PCR, restriction
| |
| - | |-
| |
| - | |MymT in pGB68
| |
| - | |MymT
| |
| - | |<partinfo>BBa_K190020</partinfo>
| |
| - | |215
| |
| - | |[http://www.neb.com/nebecomm/products/productN6707.asp pTXB1] (NEB)
| |
| - | |Ampicillin
| |
| - | |plate 3, pos. 48
| |
| - | |PCR, restriction
| |
| - | |-
| |
| - | |GlpF and fMT in pMAL-MT
| |
| - | |GlpF and fMT on a synthetic operon in the vector
| |
| - | |<partinfo>BBa_K190019</partinfo> and <partinfo>BBa_K190028</partinfo>
| |
| - | |fMT = 200, GlpF = 900?
| |
| - | |[http://www.neb.com/nebecomm/products/productN8076.asp pMALc2x] (NEB)
| |
| - | |Ampicillin
| |
| - | |plate 3, pos. 49
| |
| - | |PCR, restriction
| |
| - | |-
| |
| - | |MBP-ArsR
| |
| - | |MBP-ArsR Fusion protein
| |
| - | |<partinfo>BBa_K190027</partinfo>
| |
| - | |15180
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/chloramphenicol
| |
| - | |plate 3, pos. 53
| |
| - | |[https://2009.igem.org/Team:Groningen/Notebook/18_August_2009#Metal_accumulation Restriction analysis] & [[Team:Groningen/Notebook/25_August_2009#Metal_Accumulation| PCR]]
| |
| - | |-
| |
| - | |pNL29
| |
| - | |GVP Gene cluster
| |
| - | |{{todo|}}
| |
| - | |6000
| |
| - | |[http://www.fermentas.com/techinfo/nucleicacids/mappbluescriptiiskks.htm pBluescriptIIKS]
| |
| - | |{{todo| Ampicillin}}
| |
| - | |plate 3, pos. 54
| |
| - | |{{todo|Buoyancy phenotype}}
| |
| - | |-
| |
| - | |pLac-fMT
| |
| - | |Metallothionein with inducible promotor
| |
| - | |<partinfo>BBa_K190032</partinfo>
| |
| - | |430
| |
| - | |<partinfo>pSB1A2</partinfo>
| |
| - | |Ampicillin/chloramphenicol
| |
| - | |plate 3, pos. 55
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |pLow-fMT #4
| |
| - | |Metallothionein with low constitutive promotor
| |
| - | |<partinfo>BBa_K190031</partinfo>
| |
| - | |265
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/chloramphenicol
| |
| - | |plate 3, pos. 56
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |pLow-fMT #3
| |
| - | |Metallothionein with low constitutive promotor
| |
| - | |<partinfo>BBa_K190031</partinfo>
| |
| - | |265
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/chloramphenicol
| |
| - | |plate 3, pos. 57
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |SmtA
| |
| - | |Metallothionein
| |
| - | |<partinfo>BBa_K190021</partinfo>
| |
| - | |678
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/chloramphenicol
| |
| - | |plate 3, pos. 58
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |fMT #6
| |
| - | |Metallothionein for As<sup>3+</sup>
| |
| - | |<partinfo>BBa_K190019</partinfo>
| |
| - | |222
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/chloramphenicol
| |
| - | |plate 3, pos. 59
| |
| - | |{{todo|Sequence 2 deletions between <i>Eco</i>RI and <i>Xba</i>I sites}}
| |
| - | |-
| |
| - | |fMT #4
| |
| - | |Metallothionein for As<sup>3+</sup>
| |
| - | |<partinfo>BBa_K190019</partinfo>
| |
| - | | 222
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/chloramphenicol
| |
| - | |plate 3, pos. 60
| |
| - | |{{done|Sequence Okay!}}
| |
| - | |-
| |
| - | |pZntR-GVP
| |
| - | |Metal promoter
| |
| - | |<partinfo>BBa_K190034</partinfo>
| |
| - | |{{todo|Base Pairs (bp)}}
| |
| - | |<partinfo>pSB2K3</partinfo>
| |
| - | |Kanamycin
| |
| - | |plate 3, pos. 69
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |pCueO-GVP
| |
| - | |Metal promoter
| |
| - | |<partinfo>BBa_K190035</partinfo>
| |
| - | |{{todo|Base Pairs (bp)}}
| |
| - | |<partinfo>pSB2K3</partinfo>
| |
| - | |Kanamycin
| |
| - | |plate 3, pos. 70
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |pLacI-GVP no.1
| |
| - | |Inducible promoter
| |
| - | |<partinfo>BBa_K190036</partinfo>
| |
| - | |{{todo|Base Pairs (bp)}}
| |
| - | |<partinfo>pSB1A2</partinfo>
| |
| - | |Ampicillin
| |
| - | |plate 3, pos. 71
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |pLacI-GVP no.2
| |
| - | |Inducible promoter
| |
| - | |<partinfo>BBa_K190036</partinfo>
| |
| - | |{{todo|Base Pairs (bp)}}
| |
| - | |<partinfo>pSB1A2</partinfo>
| |
| - | |Ampicillin
| |
| - | |plate 3, pos. 72
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |High consitutive promoter(with RFP)
| |
| - | |Constitutive promoter
| |
| - | |<partinfo>Bba_J23100</partinfo>
| |
| - | |{{todo|Base Pairs (bp)}}
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/chloramphenicol
| |
| - | |plate 3, pos. 75
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |Medium consitutive promoter(with RFP)
| |
| - | |Constitutive promoter
| |
| - | |<partinfo>Bba_J23106</partinfo>
| |
| - | |{{todo|Base Pairs (bp)}}
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/chloramphenicol
| |
| - | |plate 3, pos. 76
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |Low consitutive promoter(with RFP)
| |
| - | |Constitutive promoter
| |
| - | |<partinfo>Bba_J23109</partinfo>
| |
| - | |{{todo|Base Pairs (bp)}}
| |
| - | |<partinfo>pSB1AC3</partinfo>
| |
| - | |Ampicillin/chloramphenicol
| |
| - | |plate 3, pos. 77
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |High consitutive promoter(with RFP)
| |
| - | |Constitutive promoter
| |
| - | |<partinfo>Bba_J23100</partinfo>
| |
| - | |{{todo|Base Pairs (bp)}}
| |
| - | |<partinfo>pSB3K3</partinfo>
| |
| - | |Kanamycin
| |
| - | |plate 3, pos. 78
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |Medium consitutive promoter(with RFP)
| |
| - | |Constitutive promoter
| |
| - | |<partinfo>Bba_J23106</partinfo>
| |
| - | |{{todo|Base Pairs (bp)}}
| |
| - | |<partinfo>pSB3K3</partinfo>
| |
| - | |Kanamycin
| |
| - | |plate 3, pos. 79
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |Low consitutive promoter(with RFP)
| |
| - | |Constitutive promoter
| |
| - | |<partinfo>Bba_J23109</partinfo>
| |
| - | |{{todo|Base Pairs (bp)}}
| |
| - | |<partinfo>pSB3K3</partinfo>
| |
| - | |Kanamycin
| |
| - | |plate 3, pos. 80
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |Reference constitutive promoter)
| |
| - | |Constitutive promoter
| |
| - | |?
| |
| - | |{{todo|Base Pairs (bp)}}
| |
| - | |?
| |
| - | |?
| |
| - | |plate 3, pos. 81
| |
| - | |{{todo|Quality control}}
| |
| - | |-
| |
| - | |ArsR-GvP
| |
| - | |Ars promotor + GvP gas vesicle cluster
| |
| - | |
| |
| - | |?
| |
| - | |?
| |
| - | |Kanamycin
| |
| - | |plate 3, pos. 64
| |
| - | |?
| |
| - | |}
| |
| | | | |
| | {{Team:Groningen/Footer}} | | {{Team:Groningen/Footer}} |
[http://2009.igem.org/Team:Groningen/Ethics/Survey http://2009.igem.org/wiki/images/1/1f/GroningenPrevious.png]
[http://2009.igem.org/Team:Groningen/Parts/Submitted_Parts http://2009.igem.org/wiki/images/d/dd/Next.JPG]
"
