|
|
(35 intermediate revisions not shown) |
Line 1: |
Line 1: |
| {{Team:Groningen/Header}} | | {{Team:Groningen/Header}} |
| | | |
- | <!--- The Mission, Experiments --->
| |
| | | |
- | ===Note=== | + | <div style="float:left" >{{linkedImage|GroningenPrevious.png|Team:Groningen/Ethics/Survey}}</div> |
| + | <div title="Arsie Says UP TO ACCUMULATION" style="float:right">{{linkedImage|Next.JPG|Team:Groningen/Parts/Submitted_Parts}}</div> |
| | | |
- | If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
| |
| | | |
- | :::::::::::::::http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
| |
| | | |
- | <b><h2>[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]</h2></b>
| |
| | | |
| + | *For a comprehensive list of all the parts we used, have a look at our [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen '''parts registry''']. |
| + | *Here you can find an informative overview of our [[Team:Groningen/Modelling/Submitted Parts|'''submitted parts''']]. |
| + | *Experience we had with parts from the registry can be found under [[Team:Groningen/Modelling/Used Parts|'''used parts''']]. |
| + | *Need anything specific? Take a look in our [[Team:Groningen/Parts/Freezer|freezer]] |
| | | |
- | ===Part List -80===
| |
| | | |
- | <div style="text-align: left;"> <font size="4" style="color:grey">
| |
- | <center>
| |
- | {|cellpadding="10" cellspacing="1" border="10"
| |
- | |'''Component'''
| |
- | |'''Description'''
| |
- | |'''Part or Accession #'''
| |
- | |'''Base Pairs (bp)'''
| |
- | |'''Plasmid (backbone)'''
| |
- | |'''Resistance'''
| |
- | |'''Well'''
| |
- | |'''Quality control'''
| |
- | |-
| |
- | |GVP
| |
- | |Gas Vesicle Proteins
| |
- | |[http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
| |
- | |6064
| |
- | |[http://partsregistry.org/Part:BBa_J61035 BBa_J61035]
| |
- | |Ampicillin/Gentamycin
| |
- | |plate 3, pos.1
| |
- | |?
| |
- | |-
| |
- | |GVP (2nd batch)
| |
- | |Gas Vesicle Proteins
| |
- | |[http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
| |
- | |6064
| |
- | |[http://partsregistry.org/Part:BBa_J61035 BBa_J61035]
| |
- | |Ampicillin/Gentamycin
| |
- | |plate 3, pos.2
| |
- | |?
| |
- | |-
| |
- | |pHigh in low copy nr vector
| |
- | |High consitutive promoter
| |
- | |<partinfo>J23100</partinfo>
| |
- | |35bp
| |
- | |<partinfo>pSB3K3</partinfo>
| |
- | |Kanamycin
| |
- | |Plate 3, pos. 4
| |
- | |PCR(?), restriction(+)
| |
- | |-
| |
- | |pMed in low copy nr vector
| |
- | |Medium consitutive promoter
| |
- | |<partinfo>J23106</partinfo>
| |
- | |35bp
| |
- | |<partinfo>pSB3K3</partinfo>
| |
- | |Kanamycin
| |
- | |Plate 3, pos. 5
| |
- | |PCR(?), restriction(+)
| |
- | |-
| |
- | |pLow in low copy nr vector
| |
- | |Low consitutive promoter
| |
- | |<partinfo>J23109</partinfo>
| |
- | |35bp
| |
- | |<partinfo>pSB3K3</partinfo>
| |
- | |Kanamycin
| |
- | |Plate 3, pos. 6
| |
- | |PCR(?), restriction(+)
| |
- | |-
| |
- | |pHigh in high copy nr vector
| |
- | |High consitutive promoter
| |
- | |<partinfo>J23100</partinfo>
| |
- | |35bp
| |
- | |<partinfo>pSB1AC3</partinfo>
| |
- | |Ampicillin/Chloramphenicol
| |
- | |Plate 3, pos. 7
| |
- | |PCR(?), restriction(+)
| |
- | |-
| |
- | |pMed in high copy nr vector
| |
- | |Medium consitutive promoter
| |
- | |<partinfo>J23106</partinfo>
| |
- | |35bp
| |
- | |<partinfo>pSB1AC3</partinfo>
| |
- | |Ampicillin/Chloramphenicol
| |
- | |Plate 3, pos. 8
| |
- | |PCR(?), restriction(+)
| |
- | |-
| |
- | |pLow in high copy nr vector
| |
- | |High consitutive promoter
| |
- | |<partinfo>J23109</partinfo>
| |
- | |35bp
| |
- | |<partinfo>pSB1AC3</partinfo>
| |
- | |Ampicillin/Chloramphenicol
| |
- | |Plate 3, pos. 9
| |
- | |PCR(?), restriction(+)
| |
- | |-
| |
- | |Promotor (low expression)
| |
- | |Constitutive promotor
| |
- | |[http://partsregistry.org/Part:BBa_J23109 BBa_J23109]
| |
- | |35
| |
- | |[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
| |
- | |Ampicillin
| |
- | |plate 3, pos.10
| |
- | |No
| |
- | |-
| |
- | |Promotor (medium expression)
| |
- | |Constitutive promotor
| |
- | |[http://partsregistry.org/Part:BBa_J23106 BBa_J23106]
| |
- | |35
| |
- | |[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
| |
- | |Ampicillin
| |
- | |plate 3, pos.11
| |
- | |No
| |
- | |-
| |
- | |Promotor (high expression)
| |
- | |Constitutive promotor
| |
- | |[http://partsregistry.org/Part:BBa_J23100 BBa_J23100]
| |
- | |35
| |
- | |[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
| |
- | |Ampicillin
| |
- | |plate 3, pos.12
| |
- | |No
| |
- | |-
| |
- | |Inducible promoter
| |
- | |Lactose inducible promoter nr1
| |
- | |<partinfo>R0010</partinfo>
| |
- | |200bp
| |
- | |<partinfo>pSB1A2</partinfo>
| |
- | |Ampicillin
| |
- | |Plate 3, pos. 14
| |
- | |PCR(?), restriction(+)
| |
- | |-
| |
- | |Inducible promoter
| |
- | |Lactose inducible promoter nr2
| |
- | |<partinfo>R0010</partinfo>
| |
- | |200bp
| |
- | |<partinfo>pSB1A2</partinfo>
| |
- | |Ampicillin
| |
- | |Plate 3, pos. 15
| |
- | |PCR(?), restriction(+)
| |
- | |-
| |
- | |RBS
| |
- | |Ribosomal Binding Site
| |
- | |[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]
| |
- | |12
| |
- | |[http://partsregistry.org/Part:pSB1A2 pSB1A2]
| |
- | |Ampicillin
| |
- | |plate 3, pos.19
| |
- | |No
| |
- | |-
| |
- | |Terminator
| |
- | |Double terminator consisting of [http://partsregistry.org/Part:BBa_B0012 BBa_B0012] and [http://partsregistry.org/Part:BBa_B0011 BBa_B0011]
| |
- | |[http://partsregistry.org/Part:BBa_B0014 BBa_B0014]
| |
- | |~90
| |
- | |[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| |
- | |Ampicillin/Kanamycin
| |
- | |plate 3, pos.20
| |
- | |No
| |
- | |-
| |
- | |HmtA (1.1)
| |
- | |HmtA-His in pBAD(a)
| |
- | |None
| |
- | |?
| |
- | |None
| |
- | |Ampicillin
| |
- | |plate 3, pos.28
| |
- | |Restriction(+)
| |
- | |-
| |
- | |HmtA (2.1)
| |
- | |HmtA-His in pBAD(a)
| |
- | |None
| |
- | |?
| |
- | |None
| |
- | |Ampicillin
| |
- | |plate 3, pos.29
| |
- | |Restriction(+)
| |
- | |}
| |
| | | |
- | | + | {{Team:Groningen/Footer}} |
- | | + | |
- | | + | |
- | <div align="left">
| + | |
[http://2009.igem.org/Team:Groningen/Ethics/Survey http://2009.igem.org/wiki/images/1/1f/GroningenPrevious.png]
[http://2009.igem.org/Team:Groningen/Parts/Submitted_Parts http://2009.igem.org/wiki/images/d/dd/Next.JPG]