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| {{Team:Groningen/Header}} | | {{Team:Groningen/Header}} |
| | | |
- | <!--- The Mission, Experiments --->
| |
| | | |
- | ===Note=== | + | <div style="float:left" >{{linkedImage|GroningenPrevious.png|Team:Groningen/Ethics/Survey}}</div> |
| + | <div title="Arsie Says UP TO ACCUMULATION" style="float:right">{{linkedImage|Next.JPG|Team:Groningen/Parts/Submitted_Parts}}</div> |
| | | |
- | If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
| |
| | | |
- | :::::::::::::::http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
| |
| | | |
- | <b><h2>[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]</h2></b>
| |
| | | |
| + | *For a comprehensive list of all the parts we used, have a look at our [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen '''parts registry''']. |
| + | *Here you can find an informative overview of our [[Team:Groningen/Modelling/Submitted Parts|'''submitted parts''']]. |
| + | *Experience we had with parts from the registry can be found under [[Team:Groningen/Modelling/Used Parts|'''used parts''']]. |
| + | *Need anything specific? Take a look in our [[Team:Groningen/Parts/Freezer|freezer]] |
| | | |
- | ===Part List -80===
| |
| | | |
- | {|cellpadding="10" cellspacing="1" border="10" style="font-size: large;"
| + | |
- | |'''Component'''
| + | {{Team:Groningen/Footer}} |
- | |'''Description'''
| + | |
- | |'''Part or Accession #'''
| + | |
- | |'''Base Pairs (bp)'''
| + | |
- | |'''Plasmid (backbone)'''
| + | |
- | |'''Resistance'''
| + | |
- | |'''Well'''
| + | |
- | |'''Quality control'''
| + | |
- | |-
| + | |
- | |GVP
| + | |
- | |Gas Vesicle Proteins
| + | |
- | |[http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
| + | |
- | |6064
| + | |
- | |[http://partsregistry.org/Part:BBa_J61035 BBa_J61035]
| + | |
- | |Ampicillin/Gentamycin
| + | |
- | |plate 3, pos.1
| + | |
- | |?
| + | |
- | |-
| + | |
- | |GVP (2nd batch)
| + | |
- | |Gas Vesicle Proteins
| + | |
- | |[http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
| + | |
- | |6064
| + | |
- | |[http://partsregistry.org/Part:BBa_J61035 BBa_J61035]
| + | |
- | |Ampicillin/Gentamycin
| + | |
- | |plate 3, pos.2
| + | |
- | |?
| + | |
- | |-
| + | |
- | |pHigh in low copy nr vector
| + | |
- | |High consitutive promoter
| + | |
- | |<partinfo>J23100</partinfo>
| + | |
- | |35bp
| + | |
- | |<partinfo>pSB3K3</partinfo>
| + | |
- | |Kanamycin
| + | |
- | |Plate 3, pos. 4
| + | |
- | |PCR(?), restriction(+)
| + | |
- | |-
| + | |
- | |pMed in low copy nr vector
| + | |
- | |Medium consitutive promoter
| + | |
- | |<partinfo>J23106</partinfo>
| + | |
- | |35bp
| + | |
- | |<partinfo>pSB3K3</partinfo>
| + | |
- | |Kanamycin
| + | |
- | |Plate 3, pos. 5
| + | |
- | |PCR(?), restriction(+)
| + | |
- | |-
| + | |
- | |pLow in low copy nr vector
| + | |
- | |Low consitutive promoter
| + | |
- | |<partinfo>J23109</partinfo>
| + | |
- | |35bp
| + | |
- | |<partinfo>pSB3K3</partinfo>
| + | |
- | |Kanamycin
| + | |
- | |Plate 3, pos. 6
| + | |
- | |PCR(?), restriction(+)
| + | |
- | |-
| + | |
- | |pHigh in high copy nr vector
| + | |
- | |High consitutive promoter
| + | |
- | |<partinfo>J23100</partinfo>
| + | |
- | |35bp
| + | |
- | |<partinfo>pSB1AC3</partinfo>
| + | |
- | |Ampicillin/Chloramphenicol
| + | |
- | |Plate 3, pos. 7
| + | |
- | |PCR(?), restriction(+)
| + | |
- | |-
| + | |
- | |pMed in high copy nr vector
| + | |
- | |Medium consitutive promoter
| + | |
- | |<partinfo>J23106</partinfo>
| + | |
- | |35bp
| + | |
- | |<partinfo>pSB1AC3</partinfo>
| + | |
- | |Ampicillin/Chloramphenicol
| + | |
- | |Plate 3, pos. 8
| + | |
- | |PCR(?), restriction(+)
| + | |
- | |-
| + | |
- | |pLow in high copy nr vector
| + | |
- | |High consitutive promoter
| + | |
- | |<partinfo>J23109</partinfo>
| + | |
- | |35bp
| + | |
- | |<partinfo>pSB1AC3</partinfo>
| + | |
- | |Ampicillin/Chloramphenicol
| + | |
- | |Plate 3, pos. 9
| + | |
- | |PCR(?), restriction(+)
| + | |
- | |-
| + | |
- | |Promotor (low expression)
| + | |
- | |Constitutive promotor
| + | |
- | |[http://partsregistry.org/Part:BBa_J23109 BBa_J23109]
| + | |
- | |35
| + | |
- | |[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.10
| + | |
- | |No
| + | |
- | |-
| + | |
- | |Promotor (medium expression)
| + | |
- | |Constitutive promotor
| + | |
- | |[http://partsregistry.org/Part:BBa_J23106 BBa_J23106]
| + | |
- | |35
| + | |
- | |[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.11
| + | |
- | |No
| + | |
- | |-
| + | |
- | |Promotor (high expression)
| + | |
- | |Constitutive promotor
| + | |
- | |[http://partsregistry.org/Part:BBa_J23100 BBa_J23100]
| + | |
- | |35
| + | |
- | |[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.12
| + | |
- | |No
| + | |
- | |-
| + | |
- | |Inducible promoter
| + | |
- | |Lactose inducible promoter nr1
| + | |
- | |<partinfo>R0010</partinfo>
| + | |
- | |200bp
| + | |
- | |<partinfo>pSB1A2</partinfo>
| + | |
- | |Ampicillin
| + | |
- | |Plate 3, pos. 14
| + | |
- | |PCR(?), restriction(+)
| + | |
- | |-
| + | |
- | |Inducible promoter
| + | |
- | |Lactose inducible promoter nr2
| + | |
- | |<partinfo>R0010</partinfo>
| + | |
- | |200bp
| + | |
- | |<partinfo>pSB1A2</partinfo>
| + | |
- | |Ampicillin
| + | |
- | |Plate 3, pos. 15
| + | |
- | |PCR(?), restriction(+)
| + | |
- | |-
| + | |
- | |RBS
| + | |
- | |Ribosomal Binding Site
| + | |
- | |[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]
| + | |
- | |12
| + | |
- | |[http://partsregistry.org/Part:pSB1A2 pSB1A2]
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.19
| + | |
- | |No
| + | |
- | |-
| + | |
- | |Terminator
| + | |
- | |Double terminator consisting of [http://partsregistry.org/Part:BBa_B0012 BBa_B0012] and [http://partsregistry.org/Part:BBa_B0011 BBa_B0011]
| + | |
- | |[http://partsregistry.org/Part:BBa_B0014 BBa_B0014]
| + | |
- | |~90
| + | |
- | |[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
| + | |
- | |Ampicillin/Kanamycin
| + | |
- | |plate 3, pos.20
| + | |
- | |No
| + | |
- | |-
| + | |
- | |Const. Pormoter + GVP
| + | |
- | |Low constitutive promoter with GVP cluster
| + | |
- | |[http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
| + | |
- | |~6100
| + | |
- | |[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
| + | |
- | |Ampicillin/Chloramphenicol
| + | |
- | |plate 3, pos.21
| + | |
- | |EcoRI/PstI + Sequenced
| + | |
- | |-
| + | |
- | |Const. Pormoter + GVP (no.2)
| + | |
- | |Low constitutive promoter with GVP cluster
| + | |
- | |[http://partsregistry.org/Part:BBa_J23109 BBa_J23109] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
| + | |
- | |~6100
| + | |
- | |[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
| + | |
- | |Ampicillin/Chloramphenicol
| + | |
- | |plate 3, pos.22
| + | |
- | |EcoRI/PstI + Sequenced
| + | |
- | |-
| + | |
- | |Const. Pormoter + GVP
| + | |
- | |Medium constitutive promoter with GVP cluster
| + | |
- | |[http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
| + | |
- | |~6100
| + | |
- | |[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
| + | |
- | |Ampicillin/Chloramphenicol
| + | |
- | |plate 3, pos.23
| + | |
- | |EcoRI/PstI + Sequenced
| + | |
- | |-
| + | |
- | |Const. Pormoter + GVP (no.2)
| + | |
- | |Medium constitutive promoter with GVP cluster
| + | |
- | |[http://partsregistry.org/Part:BBa_J23106 BBa_J23106] + [http://partsregistry.org/Part:BBa_I750016 BBa_I750016]
| + | |
- | |~6100
| + | |
- | |[http://partsregistry.org/Part:pSB1AC3 pSB1AC3]
| + | |
- | |Ampicillin/Chloramphenicol
| + | |
- | |plate 3, pos.24
| + | |
- | |EcoRI/PstI + Sequenced
| + | |
- | |-
| + | |
- | |HmtA (1.1)
| + | |
- | |HmtA-His in pBAD(a)
| + | |
- | |None
| + | |
- | |?
| + | |
- | |None
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.28
| + | |
- | |Restriction(+)
| + | |
- | |-
| + | |
- | |HmtA (2.1)
| + | |
- | |HmtA-His in pBAD(a)
| + | |
- | |None
| + | |
- | |?
| + | |
- | |None
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.29
| + | |
- | |Restriction(+)
| + | |
- | |-
| + | |
- | |CueO- + GVP in J61035
| + | |
- | |CueO promoter without RBS in front of the GVP cluster
| + | |
- | |None
| + | |
- | |6149
| + | |
- | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.37
| + | |
- | |Restriction(+) + Sequenced
| + | |
- | |-
| + | |
- | |ZntR- + GVP in J61035
| + | |
- | |ZntR promoter without RBS in front of the GVP cluster
| + | |
- | |None
| + | |
- | |~6150
| + | |
- | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.38
| + | |
- | |Restriction(+) + Sequenced (3 mutations)
| + | |
- | |-
| + | |
- | |ArsR- + GVP in J61035
| + | |
- | |ArsR promoter without RBS in front of the GVP cluster
| + | |
- | |None
| + | |
- | |~6150
| + | |
- | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61035 BBa_J61035] (without the part between EcoRI and XbaI)
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.39
| + | |
- | |Restriction(+) + Sequenced
| + | |
- | |-
| + | |
- | |ArsR- in J61002
| + | |
- | |pArsR (promoter without RBS)
| + | |
- | |BBa_K190015
| + | |
- | |71
| + | |
- | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.40
| + | |
- | |EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
| + | |
- | |-
| + | |
- | |ZntR- in J61002
| + | |
- | |pZntR (promoter without RBS)
| + | |
- | |BBa_K190016
| + | |
- | |65
| + | |
- | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.41
| + | |
- | |EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations)
| + | |
- | |-
| + | |
- | |CueO- in J61002
| + | |
- | |pCueO (promoter without RBS)
| + | |
- | |BBa_K190017
| + | |
- | |43
| + | |
- | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.42
| + | |
- | |EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
| + | |
- | |-
| + | |
- | |ZntR+RBS in J61002
| + | |
- | |pZntR (promoter with own RBS)
| + | |
- | |BBa_K190022
| + | |
- | |?
| + | |
- | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.43
| + | |
- | |EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
| + | |
- | |-
| + | |
- | |ArsR+RBS in J61002
| + | |
- | |pArsR (promoter with own RBS)
| + | |
- | |BBa_K190023
| + | |
- | |=
| + | |
- | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.44
| + | |
- | |EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
| + | |
- | |-
| + | |
- | |CueO~RBS in J61002
| + | |
- | |pCueO (promoter with own RBS)
| + | |
- | |BBa_K190024
| + | |
- | |
| + | |
- | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos.45
| + | |
- | |EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced
| + | |
- | |-
| + | |
- | |SmtA-GST in pGEX-3x
| + | |
- | |SmtA-GST fusion
| + | |
- | |no biobrick primers available
| + | |
- | |1400bp
| + | |
- | |pGEX-3x (NEB)
| + | |
- | |Ampicillin
| + | |
- | |plate 3, pos. 43
| + | |
- | |PCR, restriction
| + | |
- | |-
| + | |
- | |SmtA in pET29a
| + | |
- | |SmtA
| + | |
- | |<partinfo>BBa_K190021</partinfo>
| + | |
- | |900bp
| + | |
- | |pET29a (NEB)
| + | |
- | |Kanamycin
| + | |
- | |plate 3, pos. 44
| + | |
- | |PCR, restriction
| + | |
- | |-
| + | |
- | |MymT in pGB68
| + | |
- | |MymT
| + | |
- | |<partinfo>BBa_K190020</partinfo>
| + | |
- | |215bp
| + | |
- | |Ampicillin
| + | |
- | |pTXB1 (NEB)
| + | |
- | |plate 3, pos. 45
| + | |
- | |PCR, restriction
| + | |
- | |-
| + | |
- | |GlpF and fMT in pMAL-MT
| + | |
- | |GlpF and fMT on a synthetic operon in the vector
| + | |
- | |<partinfo>BBa_K190019</partinfo> and BBa_K1900?? (GlpF has no BioBrick nr yet)
| + | |
- | |fMT = 200bp, GlpF = 900bp?
| + | |
- | |Ampicillin
| + | |
- | |pMALc2x (NEB)
| + | |
- | |plate 3, pos. 46
| + | |
- | |PCR, restriction
| + | |
- | |-
| + | |
- | |MBP-ArsR
| + | |
- | |MBP-ArsR Fusion protein
| + | |
- | |{{todo|}}
| + | |
- | |1500 bp
| + | |
- | |pSB1AC3
| + | |
- | |Ampicillin/chloramphenicol
| + | |
- | |plate 3, pos. 53
| + | |
- | |[https://2009.igem.org/Team:Groningen/Notebook/18_August_2009#Metal_accumulation| Restriction analysis] & [[Team:Groningen/Notebook/25_August_2009#Metal_Accumulation| PCR]]
| + | |
- | |-
| + | |
- | |pNL29
| + | |
- | |GVP Gene cluster
| + | |
- | |{{todo|}}
| + | |
- | |6000 bp
| + | |
- | |pBluescriptIIKS
| + | |
- | |{{todo| Ampicillin}}
| + | |
- | |plate 3, pos. 54
| + | |
- | |{{todo|Buoyancy phenotype}}
| + | |
- | |-
| + | |
- | |pLac-fMT
| + | |
- | |Metallothionein with inducible promotor
| + | |
- | |{{todo|Part or Accession #}}
| + | |
- | |{{todo|Base Pairs (bp)}}
| + | |
- | |pSB1AC3
| + | |
- | |Ampicillin/chloramphenicol
| + | |
- | |plate 3, pos. 55
| + | |
- | |{{todo|Quality control}}
| + | |
- | |-
| + | |
- | |pLow-fMT #4
| + | |
- | |Metallothionein with low constitutive promotor
| + | |
- | |{{todo|Part or Accession #}}
| + | |
- | |{{todo|Base Pairs (bp)}}
| + | |
- | |pSB1AC3
| + | |
- | |Ampicillin/chloramphenicol
| + | |
- | |plate 3, pos. 56
| + | |
- | |{{todo|Quality control}}
| + | |
- | |-
| + | |
- | |pLow-fMT #3
| + | |
- | |Metallothionein with low constitutive promotor
| + | |
- | |{{todo|Part or Accession #}}
| + | |
- | |{{todo|Base Pairs (bp)}}
| + | |
- | |pSB1AC3
| + | |
- | |Ampicillin/chloramphenicol
| + | |
- | |plate 3, pos. 57
| + | |
- | |{{todo|Quality control}}
| + | |
- | |-
| + | |
- | |SmtA
| + | |
- | |Metallothionein
| + | |
- | |{{todo|Part or Accession #}}
| + | |
- | |{{todo|Base Pairs (bp)}}
| + | |
- | |pSB1AC3
| + | |
- | |Ampicillin/chloramphenicol
| + | |
- | |plate 3, pos. 58
| + | |
- | |{{todo|Quality control}}
| + | |
- | |-
| + | |
- | |fMT #6
| + | |
- | |Metallothionein
| + | |
- | |{{todo|Part or Accession #}}
| + | |
- | |{{todo|Base Pairs (bp)}}
| + | |
- | |pSB1AC3
| + | |
- | |Ampicillin/chloramphenicol
| + | |
- | |plate 3, pos. 59
| + | |
- | |{{todo|Quality control}}
| + | |
- | |}
| + | |
[http://2009.igem.org/Team:Groningen/Ethics/Survey http://2009.igem.org/wiki/images/1/1f/GroningenPrevious.png]
[http://2009.igem.org/Team:Groningen/Parts/Submitted_Parts http://2009.igem.org/wiki/images/d/dd/Next.JPG]