Team:Todai-Tokyo/Notebook/bioclock

From 2009.igem.org

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preculture of following Biobrick parts for Miniprep<BR>
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preculture of the Biobrick parts for Miniprep<BR>
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Revision as of 07:49, 9 July 2009

Plan

Aim: Create E.coli that show oscillatory gene expression pattern

Methods:
1. Clone the following genes from Bacillus subtilis into a biobrick vector.

  1. plate1,7D
  2. plate1,13B
  3. plate1,13L
  4. plate2,1H

2. Make a gene network that express oscillatory pattern, using these genes.

Cloning the parts

7/7

preculture of the Biobrick parts for Miniprep

7/8

Miniprep of E.coli cells containing LDLR gene with Promega, Wizard Plus SV Miniprep DNA Purification System

failure

7/9

Miniprep again of E.coli cells containing LDLR gene with Promega, Wizard Plus SV Miniprep DNA Purification System




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