Team:PKU Beijing

From 2009.igem.org

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<span style="font-family: Arial; font-size: 18pt">Project Summary</span><hr>
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'''A Genetic Circuit for Directed Evolution ''in vivo'''''
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==='''Conditioned Reflex Mimicking in ''E.coli'''''===
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<br><br>
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Directed evolution is a powerful tool for answering scientific questions or constructing novel biological systems. Here we present a simple genetic circuit for ''in vivo'' directed evolution which comprises minimal elements for random mutation and artificial selection. We engineer yeast to generate the DNA mutator hAID, an essential protein in adaptive immunity, and target it specifically to a gene of interest. The target gene will be mutated and consequently promptly evolves. By linking the expression of hAID repressor LacI and favorite gene functionality, the mutation rate inversely correlates between the functionality of the desired gene and hAID. This circuit may be adopted for ''in vivo'' evolution in eukaryotic system on genetically encoded targets. It has a variety of potential applications in academic and industrial contexts, theoretically most inter-molecular interaction that involves proteins and RNAs. <br>
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[https://2008.igem.org/Team:Peking_University/Project Learn More...]
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<span style="font-family: Arial; font-size: 18pt">Peking University</span><hr>
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We are engineering our E.coli cells to process the correlation information of two environmental signal, similar to the process of classical conditioning in higher organisms. In our circuits we use a bistable switch as the memory module to represent the memory state; we also constructed a series of AND gates which can sense conditioned and unconditioned signals, and output a repressor protein to change the memory state of the bistable switch. In this way, our E.coli cells can convert the information on the concurrence of two signals to its memory. After the memory module is switched, given the conditioned stimulus, E.coli will activate its reporter module and thus exhibit the "conditioned response".
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[[Image:PKU_Logic_Circuit.png|600px|center]]
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:Peking University is a comprehensive and National key university. The campus, known as "Yan Yuan"-- the gardens of Yan, is situated at the northeast of the Haidian District at the western suburbs of Beijing. It stands near the Yuan Ming Gardens and the Summer Palace.
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:The university has made an effective combination of the research on important scientific  issues with the training of personnel with high level specialized knowledge and professional skill as demanded by the country's socialist modernization. It strives not only for the simultaneous improvements in teaching and research work, but also for the promotion of interaction and mutual promotion among various subjects.
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:Peking University has become a center for teaching and research and a university of the new type,  consisting  of diverse branches of learning such as pure and applied sciences, social sciences and the humanities, and sciences of management and education. Its aim is to rank among the world's  best universities at the beginning of the next century.
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:[http://en.pku.edu.cn/ Learn More...]
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<span style="font-family: Arial; font-size: 18pt">Road to the Jamboree</span><hr>
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[[Team:PKU_Beijing/Project|Start your journey into the fancy world of Conditioned Reflex Mimicking in E.coli]]
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<div align="center"><img src="https://static.igem.org/mediawiki/2008/thumb/d/da/Time_axis2.jpg/800px-Time_axis2.jpg" align="center" width="500" height="290" border="0"  /></div>
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:[https://2008.igem.org/Team:Peking_University/Team Learn More...]
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==='''Road Map'''===
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Following is the road map of our project. We still need a second stage assembly in order to finish our project.
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You can click on the pictures to see the '''results''' of each module.So here it is.
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{{PKU_Beijing/RoadMap}}
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<span style="font-family: Arial; font-size: 18pt">Acknowledgement</span><hr>
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==='''Recommondations For Our Site'''===
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*'''Human Practice'''<BR>
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You would really want to read our report from a survey we carried out on DIYbio.
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Many similar surveys focus on what people think about, and what people would '''possibly''' do if faced with such problems. Our survey shows what people '''really''' do to DIYbio. It directly reflects the reality of the present situation that DIYbio is in.<br>
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|[[Image:1labcenter.JPG|300px]]
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[[Team:PKU_Beijing/Human/Survey|'''[Learn More]''']].
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*'''Improvement on AND Gate'''<BR>
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Our original AND Gate is from Voigt Lab. We make it into standard parts and use a combination of promoter, which leads to several AND Gates that has different properties. The most wonderful thing in the summer holiday that we achieved a greatly improved AND Gate.<br>
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|[[Image:4continetal_airlines.png|300px]]
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[[Team:PKU_Beijing/Parts_Characterization/BBa_K228258|'''[Learn More]''']].
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|[[Image:5friends.JPG|300px]]
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|[[Image:6dongsheng.JPG|300px]]
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*'''Assembly'''<BR>
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The most difficult work in the construction of a multi-Module system is assembly. After we have accomplished all the individual modules from Jul to Sept, we made a breakthrough during the National Day (we did experiments during the holiday). That is the first stage assembly is accomplished.<br>
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|[[Image:Logo_mathworks.gif]]||[[Image:NIBS.jpg|300px]]
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[[Team:PKU_Beijing/Project/Assemble|'''[Learn More]''']].
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{{PKU_Beijing/Foot}}
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__NOTOC__

Latest revision as of 12:13, 22 May 2010

News

  • We will bring our seal to jamboree, also Chinese knots as souvenir.
PKU Seal.png
  • Our T-shirt has come out:
  • First Stage Assembly accomplished, moving on!
  • Assembly between the first two Modules has been recently accomplished by Shuke Wu! More detailed work is being carried on by him!
  • Our new site is up! Feel free to send any comment to c.z.tian AT gmail.com

Countdown




Visitors

Locations of visitors to this page

 

Conditioned Reflex Mimicking in E.coli

We are engineering our E.coli cells to process the correlation information of two environmental signal, similar to the process of classical conditioning in higher organisms. In our circuits we use a bistable switch as the memory module to represent the memory state; we also constructed a series of AND gates which can sense conditioned and unconditioned signals, and output a repressor protein to change the memory state of the bistable switch. In this way, our E.coli cells can convert the information on the concurrence of two signals to its memory. After the memory module is switched, given the conditioned stimulus, E.coli will activate its reporter module and thus exhibit the "conditioned response".

PKU Logic Circuit.png


Start your journey into the fancy world of Conditioned Reflex Mimicking in E.coli

Road Map

Following is the road map of our project. We still need a second stage assembly in order to finish our project. You can click on the pictures to see the results of each module.So here it is.

Recommondations For Our Site

  • Human Practice

You would really want to read our report from a survey we carried out on DIYbio. Many similar surveys focus on what people think about, and what people would possibly do if faced with such problems. Our survey shows what people really do to DIYbio. It directly reflects the reality of the present situation that DIYbio is in.
[Learn More].


  • Improvement on AND Gate

Our original AND Gate is from Voigt Lab. We make it into standard parts and use a combination of promoter, which leads to several AND Gates that has different properties. The most wonderful thing in the summer holiday that we achieved a greatly improved AND Gate.
[Learn More].


  • Assembly

The most difficult work in the construction of a multi-Module system is assembly. After we have accomplished all the individual modules from Jul to Sept, we made a breakthrough during the National Day (we did experiments during the holiday). That is the first stage assembly is accomplished.
[Learn More].




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