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Team:UNC Chapel Hill/2 July 2009
From 2009.igem.org
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| + | [[Team:UNC_Chapel_Hill/Notebook|Back]] | ||
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==Notes== | ==Notes== | ||
| - | *Pavel and Joe took the samples of the bacteria cultures with the RFP part and the LacY part, pelleted them, and ran them in gel. | + | *Pavel and Joe took the samples of the bacteria cultures with the RFP part and the LacY part, pelleted them, and ran them in gel. |
| + | **[http://uncbme.com/uploads/7209MiniprepRFPandLacY.jpg Image of gel under UV] | ||
| + | ***Ladder, Positive Control, RFP, LacY | ||
*Kar, Evan, Pavel, and Joe prepared stock of the parts as follow and then transformed them into the bacteria: | *Kar, Evan, Pavel, and Joe prepared stock of the parts as follow and then transformed them into the bacteria: | ||
| - | ** | + | **[http://partsregistry.org/Part:BBa_J23101 Promoter] - Plate 1 |
| - | ** | + | **[http://partsregistry.org/Part:BBa_C0012 LacI] - Plate 2 |
| - | ** | + | **[http://partsregistry.org/Part:BBa_R0011 LacI Promoter] - Plate 3 |
| + | **pBluescript (positive control) - Plate 4 | ||
Latest revision as of 14:46, 9 July 2009
Notes
- Pavel and Joe took the samples of the bacteria cultures with the RFP part and the LacY part, pelleted them, and ran them in gel.
- [http://uncbme.com/uploads/7209MiniprepRFPandLacY.jpg Image of gel under UV]
- Ladder, Positive Control, RFP, LacY
- [http://uncbme.com/uploads/7209MiniprepRFPandLacY.jpg Image of gel under UV]
- Kar, Evan, Pavel, and Joe prepared stock of the parts as follow and then transformed them into the bacteria:
- [http://partsregistry.org/Part:BBa_J23101 Promoter] - Plate 1
- [http://partsregistry.org/Part:BBa_C0012 LacI] - Plate 2
- [http://partsregistry.org/Part:BBa_R0011 LacI Promoter] - Plate 3
- pBluescript (positive control) - Plate 4
