Team:Wash U/Calendar-Home/7 July 2009

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== Tuesday July 7, 2009 ==
== Tuesday July 7, 2009 ==
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- Ran [https://static.igem.org/mediawiki/2009/f/f0/7-7_ligation_products.jpg Gel] of digestion and ligation products from Biobrick Assembly <br>
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- Made glycerol stock solutions: <br>
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- Performed colony PCR on colonies transformed with plasmid from ligation reaction <br>
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Results: [https://static.igem.org/mediawiki/2009/b/b6/Colony_PCR_7-8.jpg Gel] [https://static.igem.org/mediawiki/2009/9/9f/8_colonies_from_colony_PCR.jpg Colonies] <br>
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Red colonies indicate incorrect ligation <br>
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- Made [https://2009.igem.org/Team:Wash_U/Protocol#Glycerol_Stock_Solution glycerol stock solutions]: <br>
1.  pSB1AC3 <br>
1.  pSB1AC3 <br>
2.  puc19 E. coli <br>
2.  puc19 E. coli <br>
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14. pSB1K3 <br>
14. pSB1K3 <br>
15. R. sphaeroides 2.4.1 <br>
15. R. sphaeroides 2.4.1 <br>
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-Purified genomic DNA of E. coli and R. sphaeroides for PCR <br>
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DNA Quantities: <br>
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1. E.coli (pellet from 1.5 mL of culture): 54.5 ng/uL <br>
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2. E.coli (pellet from 3.0 mL of culture): 70.0 ng/uL <br>
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3. R.sphaeroides (from 3.0 mL of culture): 62.5 ng/uL <br>
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4. R.sphaeroides (from 1.5 mL of culture): 40.5 ng/uL <br>
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Latest revision as of 16:45, 9 July 2009


Tuesday July 7, 2009

- Ran Gel of digestion and ligation products from Biobrick Assembly

- Performed colony PCR on colonies transformed with plasmid from ligation reaction
Results: Gel Colonies
Red colonies indicate incorrect ligation

- Made glycerol stock solutions:
1. pSB1AC3
2. puc19 E. coli
3. BBa_I15010 pSB2K3
4. S17-1 E. coli
5. BBa_R0082 pSB1A2
6. pSB1T3
7. BBa_E0240 pSB1A2
8. BBa_B0034 pSB1A2
9. pSB1AT3
10. pRKCBC3
11. pSB1A3
12. BBa_B0015 pSB1AK3
13. pucHS27
14. pSB1K3
15. R. sphaeroides 2.4.1

-Purified genomic DNA of E. coli and R. sphaeroides for PCR
DNA Quantities:
1. E.coli (pellet from 1.5 mL of culture): 54.5 ng/uL
2. E.coli (pellet from 3.0 mL of culture): 70.0 ng/uL
3. R.sphaeroides (from 3.0 mL of culture): 62.5 ng/uL
4. R.sphaeroides (from 1.5 mL of culture): 40.5 ng/uL



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