Virginia Commonwealth/9 July 2009
From 2009.igem.org
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===Tasks=== | ===Tasks=== | ||
''Afton and Maria'' | ''Afton and Maria'' | ||
- | * Pick cells for overnight culture | + | * Pick cells for overnight culture |
- | + | [[User:Trentay|Trentay]] 19:45, 9 July 2009 (UTC) | |
- | [[User:Trentay|Trentay]] | + | |
''Kevin and Adam'' | ''Kevin and Adam'' | ||
* Back bone pSB3K3 is a bad part. Cultivate backbone pSB1C3 from glycerol stock. | * Back bone pSB3K3 is a bad part. Cultivate backbone pSB1C3 from glycerol stock. |
Revision as of 19:45, 9 July 2009
Contents |
Thursday 9 July 2009
Results
Afton and Maria
- Electrotransformation was successful
- Control (+) NEB 10 beta on LB has lawn growth
- Control (+) NEB 10 beta on LB electroshocked has lawn growth
- Control (-) NEB 10 beta on AMP has no growth
- J23106 (promoter) on AMP has hundreds of little colonies
- J06702 (RET) on AMP has about one hundred colonies
Trentay 14:06, 9 July 2009 (UTC)
Tasks
Afton and Maria
- Pick cells for overnight culture
Trentay 19:45, 9 July 2009 (UTC)
Kevin and Adam
- Back bone pSB3K3 is a bad part. Cultivate backbone pSB1C3 from glycerol stock.
- Proceed with analytical work and ordering DNA.
Wetlab
Afton and Maria
- 1
- 2
Trentay 14:06, 9 July 2009 (UTC)