Team:Wash U/Calendar-Home/9 July 2009

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==Thursday July 9, 2009==
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-Ran 2% agarose gel at 115 volts to visualize OmpC-GFP ligation product and proper digests
-Ran 2% agarose gel at 115 volts to visualize OmpC-GFP ligation product and proper digests
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-Transformed cph8 from the Voight Lab into GC5 E. coli
-Transformed cph8 from the Voight Lab into GC5 E. coli
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Latest revision as of 18:02, 10 July 2009


Thursday July 9, 2009

-Ran 2% agarose gel at 115 volts to visualize OmpC-GFP ligation product and proper digests

-Performed fluorescence spectrophotometry to verify OmpC-GFP ligation success

      -Results were not significantly different from wild-type E. coli

-Submitted OmpC-GFP ligation plasmid for sequencing to verify success of biobrick assembly protocol

-Prepared LB media and autoclaved glassware

-Transformed cph8 from the Voight Lab into GC5 E. coli


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