Team:UNC Chapel Hill/Project Needs

From 2009.igem.org

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m (Project Needs moved to Team:UNC Chapel Hill/Project Needs: Put in team directory)
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==Basic Lab Supplies==
==Basic Lab Supplies==
# Blank Petri Dishes
# Blank Petri Dishes
 +
# Electric cuvettes
 +
# Spin column tubes for MiniPrep
# Disposable Gloves (S, M, L)
# Disposable Gloves (S, M, L)
# Screw Top Microcentrifuge Tubes (1.5 mL)
# Screw Top Microcentrifuge Tubes (1.5 mL)
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# PCR Supermix
# PCR Supermix
# Competent Cells (DH10B, Top10, or DH5a.  DB3.1)
# Competent Cells (DH10B, Top10, or DH5a.  DB3.1)
 +
==Equipment==
==Equipment==

Revision as of 22:01, 10 July 2009

Basic Lab Supplies

  1. Blank Petri Dishes
  2. Electric cuvettes
  3. Spin column tubes for MiniPrep
  4. Disposable Gloves (S, M, L)
  5. Screw Top Microcentrifuge Tubes (1.5 mL)
  6. Cell Culture Tubes (10 mL)
  7. Eppendorf Tubes (1.5 mL)
  8. Glass Pasteur pipets (9 mL)
  9. Parafilm
  10. Micropipets (P2, P20, P200, P1000)
  11. Micropipet Tips (for all sizes listed above)
  12. Restriction Enzymes-if from Fermentas, should be FastDigest versions. If NEB, we need EcoRI-HF:
    1. EcoRI
    2. Xbal
    3. SpeI
    4. PstI
    5. MunI (MfeI)
    6. Mph1103I (NsiI)
  13. T4 DNA Ligase
  14. Agarose Powder
  15. Luria Broth and/or Super Optimal Broth
  16. Buffer for Gel Electrophoresis
  17. Antibiotics:
    1. Carbenicillin
    2. Kanamycin
  18. Gel Extraction Kit
  19. PCR Supermix
  20. Competent Cells (DH10B, Top10, or DH5a. DB3.1)


Equipment

  1. Thermal Cycler
  2. Fluorometer

Other

  1. Plane tickets to Boston for Jamboree weekend (October 31 to November 2nd, 2009)
  2. Hotel stay in Boston for Jamboree
  3. Attendance fee for Jamboree ($175 per student; $375 per advisor)
  4. Custom T-shirts
Retrieved from "http://2009.igem.org/Team:UNC_Chapel_Hill/Project_Needs"