Virginia Commonwealth/9 July 2009

From 2009.igem.org

(Difference between revisions)
(Wetlab)
(Tasks)
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''Kevin and Adam''
''Kevin and Adam''
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* Back bone pSB3K3 is a bad part. Cultivate backbone pSB1C3 from glycerol stock.
 
* Proceed with analytical work and ordering DNA.
* Proceed with analytical work and ordering DNA.
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Revision as of 16:35, 13 July 2009

Contents

Thursday 9 July 2009

Results

Afton and Maria

  • Electrotransformation was successful
    • Control (+) NEB 10 beta on LB has lawn growth
    • Control (+) NEB 10 beta on LB electroshocked has lawn growth
    • Control (-) NEB 10 beta on AMP has no growth
    • J23106 (promoter) on AMP has hundreds of little colonies
    • J06702 (RET) on AMP has about one hundred colonies

Trentay 14:06, 9 July 2009 (UTC)


Tasks

Afton and Maria

  • Pick cells for overnight culture

Trentay 19:45, 9 July 2009 (UTC)

Kevin and Adam

  • Proceed with analytical work and ordering DNA.

Wetlab

Afton and Maria

  • Put 5 colonies of J23106 in 5mL of LB (two test tube)
  • Put 5 colonies of J06702 in 5mL of LB (one test tube)

Trentay 21:13, 9 July 2009 (UTC)