Team:Wash U/Calendar-Home/14 July 2009

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== Tuesday, July 14, 2009 ==
-Colony PCR of cells transformed with previous ligations<br>
-Colony PCR of cells transformed with previous ligations<br>
-PCR to add KpnI and BamHI sites to GFP <br>
-PCR to add KpnI and BamHI sites to GFP <br>

Revision as of 20:16, 14 July 2009

Tuesday, July 14, 2009

-Colony PCR of cells transformed with previous ligations
-PCR to add KpnI and BamHI sites to GFP
-Spectrophotometry of dbcOmega strains in ATCC media
-PCR to add BioBrick prefix and suffix to Cph8 and pRKCBC3
-Transformed S17 competent cells to check efficiency. OD = 0.115
-Performed Triligation and Silver Lab Ligations to fuse OmpC+GFP
-Started cultures of dbcOmega in LB+Strep in lieu of ATCC


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