Team:Wash U/Biological Parts
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<br><br><br><br><br><br><br><br><br>[[Image:Slide4.jpg|480px|left]] Part three of our characterization measures the effectiveness of the red light sensor in phosphorylating ompR. This setup is the exact same as in part two except that we have added in the red light sensor. Now, ompR may be phosphorylated by the puc promoter or the red light sensor instead of solely by the puc promoter. GFP is still attached to the end product, the ompC promoter. By comparing the fluorescence of GFP in this scenario compared with the second scenario we will see how much more ompR was phosphorylated with the red light sensor that without. | <br><br><br><br><br><br><br><br><br>[[Image:Slide4.jpg|480px|left]] Part three of our characterization measures the effectiveness of the red light sensor in phosphorylating ompR. This setup is the exact same as in part two except that we have added in the red light sensor. Now, ompR may be phosphorylated by the puc promoter or the red light sensor instead of solely by the puc promoter. GFP is still attached to the end product, the ompC promoter. By comparing the fluorescence of GFP in this scenario compared with the second scenario we will see how much more ompR was phosphorylated with the red light sensor that without. | ||
<br><br><br><br><br><br><br><br><br><br><br><br><br>[[Image:Slide5.jpg|480px|left]] This is the final construct that will be our actual functioning model in ''Rhodobacter sphaeroides''. This finished product will be compared to the wild type over various intensities of light and cell culture densities can be compared to see which strain, the wild type or the modified strain with the red light sensor was more efficient in harvesting light with varying intensities. | <br><br><br><br><br><br><br><br><br><br><br><br><br>[[Image:Slide5.jpg|480px|left]] This is the final construct that will be our actual functioning model in ''Rhodobacter sphaeroides''. This finished product will be compared to the wild type over various intensities of light and cell culture densities can be compared to see which strain, the wild type or the modified strain with the red light sensor was more efficient in harvesting light with varying intensities. | ||
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Revision as of 03:09, 15 July 2009