Team:Wash U/Calendar-Home/14 July 2009

From 2009.igem.org

(Difference between revisions)

Latest revision as of 16:43, 15 July 2009

Tuesday, July 14, 2009

-Colony PCR of cells transformed with previous ligations
-PCR to add KpnI and BamHI sites to GFP
-Spectrophotometry of dbcOmega strains in ATCC media
-PCR to add BioBrick prefix and suffix to Cph8 and pRKCBC3
-Transformed S17 competent cells to check efficiency. OD = 0.115
-Performed Triligation and Silver Lab Ligations to fuse OmpC+GFP
-Started cultures of dbcOmega in LB+Strep in lieu of ATCC

Gel #1: Silver digestion protocol

1) Ladder

2) OmpC + pSB1A2 plasmid (2187 bp)

3) OmpC + pSB1A2 plasmid

4) GFP (876 bp)

5) GFP

6) Ladder

Gel #2: BioBrick (Triligation) digestion protocol

1) Ladder

2) OmpC (108 bp)

3) OmpC

4) Destination plasmid, pSB1K3 (2206 bp)

5) Dest. plasmid, pSB1K3

6) GFP (876 bp)

7) GFP

8) Ladder

Back to Calendar

@@ Line 10: / Line 10: @@
 -Started cultures of dbcOmega in LB+Strep in lieu of ATCC <br>
-Gel #1: Silver
+Gel #1: Silver digestion protocol
 ) Ladder
-) #1 OmpC
+) OmpC + pSB1A2 plasmid (2187 bp)
-) #1 OmpC
+) OmpC + pSB1A2 plasmid
-) #1 GFP
+) GFP (876 bp)
-) #1 GFP
+) GFP
 ) Ladder
-Gel #2: BioBrick
+Gel #2: BioBrick (Triligation) digestion protocol
 ) Ladder
-) #2 OmpC
+) OmpC (108 bp)
-) #2 OmpC
+) OmpC
-) #2 Destination plasmid
+) Destination plasmid, pSB1K3 (2206 bp)
-) #2 Dest. plasmid
+) Dest. plasmid, pSB1K3
-) #2 GFP
+) GFP (876 bp)
-) #2 GFP
+) GFP
 ) Ladder
@@ Line 45: / Line 45: @@
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-[[Team:Wash_U/Calendar-Home/9 July 2009|Previous entry]]</div>
+[[Team:Wash_U/Calendar-Home/13 July 2009|Previous entry]]</div>
-  [[Team:Wash_U/Calendar-Home/14 July 2009|Next entry]]</div>
+  [[Team:Wash_U/Calendar-Home/15 July 2009|Next entry]]</div>
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