Team:Wash U/Calendar-Home/1 July 2009

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== Wednesday July 1, 2009 ==
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-Miniprep #2 to obtain more biobrick DNA
-Miniprep #2 to obtain more biobrick DNA
-New gel with digested vs. undigested plasmids
-New gel with digested vs. undigested plasmids
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-Started new restriction digests on plasmids (single cut with enzyme #1, single cut with enzyme #2, double cut); will run these on gel tomorrow
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-Started new overnight cultures of biobrick parts and plasmids
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-Team meeting #4
Miniprep #2:
Miniprep #2:
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<table>
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<tr>
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<td>
1) BBa_I15010  pSB2K3
1) BBa_I15010  pSB2K3
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12) plasmid pRKCBC3
12) plasmid pRKCBC3
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<td>
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35 ng/ul
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45 ng/ul
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used
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62.5 ng/ul
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42 ng/ul
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28.5 ng/ul
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133.0 ng/ul
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62.5 ng/ul
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54.0 ng/ul
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64.5 ng/ul
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31.5 ng/ul
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45.5 ng/ul
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</table>
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<pre>
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Note:  Nanospec: Used 3ul on Lid 10, 320nm background ON, blank after each measurement with TE elution buffer (Sigma)
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*Measured again with EtOH wipe after each measurement 7-15
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</pre>
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<div style="text-align: right;">
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[[Team:Wash_U/Calendar-Home/30 June 2009|Previous entry]]</div>
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[[Team:Wash_U/Calendar-Home/2 July 2009|Next entry]]</div>
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<center>[[Team:Wash U/Notebook|Back to Calendar]]</center>
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{{WashUbottom}}

Latest revision as of 21:12, 15 July 2009



Wednesday July 1, 2009

-Miniprep #2 to obtain more biobrick DNA

-New gel with digested vs. undigested plasmids

-Started new restriction digests on plasmids (single cut with enzyme #1, single cut with enzyme #2, double cut); will run these on gel tomorrow

-Started new overnight cultures of biobrick parts and plasmids

-Team meeting #4

Miniprep #2:

1) BBa_I15010 pSB2K3

2) BBa_E0240 pSB1A2

3) BBa_R0082 pSB1A2

4) plasmid pSB1T3

5) plasmid pSB1K3

6) BBa_B0034 pSB1A2

7) plasmid pSB1AT3

8) plasmid pSB1A3

9) plasmid pucHS27

10) plasmid pSB1AC3

11) BBa_B0015 pSB1AK3

12) plasmid pRKCBC3

35 ng/ul

45 ng/ul

used

62.5 ng/ul

42 ng/ul

28.5 ng/ul

133.0 ng/ul

62.5 ng/ul

54.0 ng/ul

64.5 ng/ul

31.5 ng/ul

45.5 ng/ul 

Note:  Nanospec: Used 3ul on Lid 10, 320nm background ON, blank after each measurement with TE elution buffer (Sigma)
*Measured again with EtOH wipe after each measurement 7-15


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