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Team:UCL London/Project/Description
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'''“Happy Living”''' | '''“Happy Living”''' | ||
| - | Our project is aiming to produce a series of biosensors which can improve the traditional mechanical measurements in bio-processing by using the different fluorescent proteins as indicators of different stresses for bacteria during the bio-processing, particularly on oxygen level, acetate production, shear stress, and cell growth phase. | + | Our project is aiming to produce a series of biosensors which can improve the traditional mechanical measurements in bio-processing by using the different fluorescent proteins as indicators of different stresses for bacteria during the bio-processing, particularly on oxygen level, acetate production/concentration, shear stress, and cell growth phase. |
| - | Experiments are based on manipulating ''E.coli'' cells. Standard parts from the registry will be joined with different promoters from natural/modified E.coli genome which respond to reducing oxygen level, mis-folding proteins, and cell growth. The modified standard plasmids are then transformed into ''E.coli K12'' cells | + | Experiments are based on manipulating ''E.coli'' cells. Standard parts from the registry will be joined with different promoters from natural/modified E.coli genome, or alternatively response mechanisms from other organisms, which respond to reducing oxygen level, mis-folding proteins, and cell growth. The modified standard plasmids are then transformed into ''E.coli K12'' cells for subsequent investigation in both wet lab and fermentation processes. Once the individual operons are functioning well, related operons can be assembled as a cassette for detection of a range of stresses for bacteria. |
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| - | Although biosensors are now widely used in synthetic biology area, what make our project unique is that we focus on the ‘feeling’ of bacteria themselves | + | Although biosensors are now widely used in synthetic biology area, what make our project unique is that we focus on the ‘feeling’ of bacteria themselves. Detecting why a bacteria is not growing well or dying and with that knowledge change a parameter to make them happier can enable the bacterias to manufacture a lot more useful bioproducts for us humans. |
Revision as of 09:01, 16 July 2009
“Happy Living”
Our project is aiming to produce a series of biosensors which can improve the traditional mechanical measurements in bio-processing by using the different fluorescent proteins as indicators of different stresses for bacteria during the bio-processing, particularly on oxygen level, acetate production/concentration, shear stress, and cell growth phase.
Experiments are based on manipulating E.coli cells. Standard parts from the registry will be joined with different promoters from natural/modified E.coli genome, or alternatively response mechanisms from other organisms, which respond to reducing oxygen level, mis-folding proteins, and cell growth. The modified standard plasmids are then transformed into E.coli K12 cells for subsequent investigation in both wet lab and fermentation processes. Once the individual operons are functioning well, related operons can be assembled as a cassette for detection of a range of stresses for bacteria.
Apart from the wet lab, modelling is also a very important part in the project …
Although biosensors are now widely used in synthetic biology area, what make our project unique is that we focus on the ‘feeling’ of bacteria themselves. Detecting why a bacteria is not growing well or dying and with that knowledge change a parameter to make them happier can enable the bacterias to manufacture a lot more useful bioproducts for us humans.
