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Team:KULeuven/Modelling
From 2009.igem.org
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The purpose of the sensor is to increase the production of the RIBOKEY under the control of the intensity of the incident blue light. | The purpose of the sensor is to increase the production of the RIBOKEY under the control of the intensity of the incident blue light. | ||
| - | Input: Blue light (<math>lambda = 470</math>) <br> | + | Input: Blue light (<math>\lambda = 470</math>) <br> |
Output: mRNA_RIBOKEY | Output: mRNA_RIBOKEY | ||
[[Image:black_box_light_sensor.JPG|center|thumb|300px|Sensor interface]] | [[Image:black_box_light_sensor.JPG|center|thumb|300px|Sensor interface]] | ||
| + | |||
| + | The biology works as follows, YcgF protein is believed to dimerize upon photo-excitation. | ||
| + | The YcgE protein is a repressor for the transcription of the mRNA_RIBOKEY, only the dimer form of YcgF is capable of | ||
| + | interacting with the operator-bound YcgE and to release it from the DNA. | ||
| + | |||
| + | The entire network of interactions is visualized in the diagram below | ||
[[Image:blue_light_sensor.jpg|center|thumb|630px|Regulation of the production of mRNA key by incident blue light]] | [[Image:blue_light_sensor.jpg|center|thumb|630px|Regulation of the production of mRNA key by incident blue light]] | ||
Revision as of 11:46, 16 July 2009
Blue Light Sensor
The purpose of the sensor is to increase the production of the RIBOKEY under the control of the intensity of the incident blue light.
Input: Blue light (<math>\lambda = 470</math>)
Output: mRNA_RIBOKEY
The biology works as follows, YcgF protein is believed to dimerize upon photo-excitation. The YcgE protein is a repressor for the transcription of the mRNA_RIBOKEY, only the dimer form of YcgF is capable of interacting with the operator-bound YcgE and to release it from the DNA.
The entire network of interactions is visualized in the diagram below
References
[1] Natalia Tschowri, Susan Busse and Regine Hengge, "The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli"
