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13 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: '''Aim:''' * Ligation of tetR promoter and YFP, GFP, CFP. '''Materials:''' {| border="1" align="center" !align="center"| !! align="center"|Control 1 !! align="center"|Control 2 !! al...) |
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| + | # Add all the contents above into labelled eppendorfs, and spin down. | ||
| + | # Leave at room temperature for 30 min in order to fully ligate. | ||
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Latest revision as of 12:40, 16 July 2009
Aim:
- Ligation of tetR promoter and YFP, GFP, CFP.
Materials:
| Control 1 | Control 2 | YFP | GFP | CFP | |
|---|---|---|---|---|---|
| Ligase Buffer | 10 | 10 | 10 | 10 | 10 |
| Quick Ligase | 1 | 0 | 1 | 1 | 1 |
| dH2O | 6 | 7 | --- | --- | --- |
| Backbone | 3 | 3 | 3 | 3 | 3 |
| Promoter | --- | --- | 3 | 3 | 3 |
| FP | --- | --- | 3 | 3 | 3 |
| Total Volume | 20 | 20 | 20 | 20 | 20 |
Method:
- Add all the contents above into labelled eppendorfs, and spin down.
- Leave at room temperature for 30 min in order to fully ligate.
