"
Virginia Commonwealth/16 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: ==Saturday 06 June 2009== ===Results=== * Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation {| cellpadding="10" align="center" border="0.9" padd...) |
|||
| Line 51: | Line 51: | ||
===Tasks=== | ===Tasks=== | ||
''Maria and Afton'' | ''Maria and Afton'' | ||
| - | * Digest: E0240, pSB4C5, 110, 113, 116, | + | * Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040 |
| + | * Ligate promoters with backbone pSB4C5 and reporters E0240, J06702 | ||
| + | * Run an electrophoresis test on promoters and reporter Eo240 | ||
| + | * Pick colonies of promoters from transformation 100-107 (excluding 106) | ||
| + | * Transformation of NEB 10 beta to make sure new stock is electrocompetent | ||
| + | [[User:Trentay|Trentay]] 20:41, 16 July 2009 (UTC) | ||
---- | ---- | ||
====Wetlab==== | ====Wetlab==== | ||
''Maria and Afton'' | ''Maria and Afton'' | ||
| - | * | + | * Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040 |
| + | * Ligate promoters with backbone pSB4C5 and reporters E0240, J06702 | ||
| + | * Run an electrophoresis test on promoters and reporter Eo240 | ||
| + | [[User:Trentay|Trentay]] 20:41, 16 July 2009 (UTC) | ||
Revision as of 20:41, 16 July 2009
Contents |
Saturday 06 June 2009
Results
- Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation
| Promoter | Reporter | Resistance/Plate | Growth Observation | |
|---|---|---|---|---|
| J23101 | E0240 | Cm | no growth | |
| J23101 | J06702 | Cm | 21 colonies | |
| J23102 | E0240 | Cm | 3 colonies | |
| J23102 | J06702 | Cm | 8 colonies | |
| J23103 | E0240 | Cm | 2 colonies | |
| J23103 | J06702 | Cm | 23 colonies | |
| J23104 | E0240 | Cm | 9 colonies | |
| J23104 | J06702 | Cm | 12 colonies | |
| J23105 | E0240 | Cm | no growth | |
| J23105 | J06702 | Cm | 14 colonies | |
| J23107 | E0240 | Cm | 3 colonies | |
| J23107 | J06702 | Cm | 3 colonies |
- No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
- Vial may have thawed out too much after cells were taken out for the electrocompetency procedure
Trentay 14:31, 16 July 2009 (UTC)
Tasks
Maria and Afton
- Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
- Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
- Run an electrophoresis test on promoters and reporter Eo240
- Pick colonies of promoters from transformation 100-107 (excluding 106)
- Transformation of NEB 10 beta to make sure new stock is electrocompetent
Trentay 20:41, 16 July 2009 (UTC)
Wetlab
Maria and Afton
- Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
- Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
- Run an electrophoresis test on promoters and reporter Eo240
Trentay 20:41, 16 July 2009 (UTC)
