Wiki/Team:Warsaw/primers

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<h1>Primers</h1>
<h1>Primers</h1>
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<h2>'One-armed bandit' strain preparation</h2>
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<h2>cloning primers</h2>
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<h4>AID and T7 cloning</h4>
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<h4>cloning primers</h4>
<pre>
<pre>
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<a name="T7lLinkB">T7lLinkB</a>  5' CGGATCCGGCGGTGGCGGTAGTGGAGGAGGTGGGTCTGGAGGCGGAGGGAACACGATTAACATCGCTAAGAAC 3'
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<a name="croboxF">croboxF</a>  5' ATCTAGATACCTCTGGCGGTGATACTAGTGT 3'
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<a name="T7lRBSHi">T7lRBSHi</a>  5' CACAAGCTTAGGAGGTGTACTAGATGAACACGATTAACATCGCTAAG 3'
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<a name="croboxR">croboxR</a>  5' ACACTAGTATCACCGCCAGAGGTATCTAGAT 3'
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<a name="T7pXbSal">T7pXbSal</a>   5' TCTGTCGACTCTAGATTACGCGAACGCGAAGTCC 3'
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<a name="invF">invF</a>     5' ATCTAGATTATATTGACAGCGCACAGAGCGGGAA 3'
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<a name="AIDlNrH">AIDlNrH</a>   5' CGTAAGCTTAGGAGGTGTAGACCATGGACAGCCTCTTGATG 3'
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<a name="invR">invR</a>     5' GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT 3'
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<a name="AIDpLinB">AIDpLinB</a>   5' CCACTACCGCCACCGCCGGATCCGCCACCTCCGCTGCCTCCACCACCAAGTCCCAAAGTACGAAATGC 3'
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<a name="hlyF">hlyF</a>     5' TGTCTAGAATGAAAAAAATAATGCTAGTT  3'
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<a name="hlyR">hlyR</a>      5' GACTAGTTTATTCGATTGGATTATCTA  3'
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<a name="croF">croF</a>      5' ATCTAGAATGGAACAACGCATAACC  3'
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<a name="croR">croR</a>      5' GTACTAGTGGGCTGGAATGTGTAAG  3'
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<a name="phoF">phoF</a>      5' ATCTAGAATGATGCGCGTACTGGTTGTAG  3'
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<a name="phoR">phoR</a>      5' TTACTAGTGATGCGTGGAAGAACGCACAG  3'
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<a name="mgtCF">mgtCF</a>    5' ATCTAGATCCATTTTTTCTGGAAGAATAAG  3'
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<a name="mgtCR">mgtCR</a>    5' GTACTAGTCCTCGTCATGTTGTTTTATTTTTT 3'
</pre>
</pre>

Latest revision as of 10:30, 18 July 2009


Return to notebookReturn to the previous page

Primers

cloning primers

cloning primers

croboxF   5' ATCTAGATACCTCTGGCGGTGATACTAGTGT  3'
croboxR   5' ACACTAGTATCACCGCCAGAGGTATCTAGAT  3'
invF      5' ATCTAGATTATATTGACAGCGCACAGAGCGGGAA  3'
invR      5' GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT  3'
hlyF      5' TGTCTAGAATGAAAAAAATAATGCTAGTT  3'
hlyR      5' GACTAGTTTATTCGATTGGATTATCTA  3'
croF      5' ATCTAGAATGGAACAACGCATAACC  3'
croR      5' GTACTAGTGGGCTGGAATGTGTAAG  3'
phoF      5' ATCTAGAATGATGCGCGTACTGGTTGTAG  3'
phoR      5' TTACTAGTGATGCGTGGAAGAACGCACAG  3'
mgtCF     5' ATCTAGATCCATTTTTTCTGGAAGAATAAG  3'
mgtCR     5' GTACTAGTCCTCGTCATGTTGTTTTATTTTTT  3'


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