Team:Illinois/Vectors

From 2009.igem.org

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== '''Gels'''==
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This is the first gel of the PCR that we did of the pJU-334 DNA sequence. The band shows up at ~1.9 kbp. We expected it to be at 3.1 kbp.
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[[Image:Illinois-_Gel_1_of_PCR_of_pJU-334.jpg]]
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This is the second gel of the PCR that we did of the pJU-334 DNA sequence. The band shows up at ~11 kbp --oops. Again, we expected a band at ~3.1 kbp.
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[[Image:Illinois-_Gel_2_of_PCR_of_pJU-334.jpg]]
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This is the third gel of the PCR that we did of the pJU-334 DNA sequence. Here we see two band. A faint one, (where we expected the band to show up) at 3.1 kbp. The second, more prominent band is at ~1.9 kpb again.
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[[Image:Illinois-_Gel_3_of_pJU-334.jpg]]
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This is the fourth gel of the PCR that we did of the pJU-334 DNA sequence. This time, we put in all 45 micro liters of the DNA. Again, we got fainter bands at 3.1 kbp and stronger ones at ~1.9. Then we purified the 3.1 kbp fragments of DNA. We then used this purified DNA for another PCR. Next, we intend to run a gel of this, isolate the 3.1 kbp fragment, and use this to proceed with our experiment.
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[[Image:Illinois-_Gel_4_of_pJU-334.jpg]]
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Update: We just decided that maybe they told us to use the worng primer for the anit-sense (they had gotten rid of part of the plasmid). They gave us the primer pLlac-OB and we were supposed to use plLac-OD.
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So the question becomes, how come we got DNA fragments that were about the right size? Maybe the primers similar?
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== '''Vectors''' ==
== '''Vectors''' ==
This page contains maps of the plasmids used in our project.
This page contains maps of the plasmids used in our project.
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== '''pJU-334 Plasmid''' ==
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== '''PJU-334 Plasmid''' ==
 
[[Image:pju-334_map.png]]
[[Image:pju-334_map.png]]
Note: The PZE12luc plasmid is the parental plasmid of the PJU-334 linear fragment.
Note: The PZE12luc plasmid is the parental plasmid of the PJU-334 linear fragment.
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== '''PXG-10 Plasmid''' ==
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== '''pXG-10 Plasmid''' ==
[[Image:pxg-10_map.png]]
[[Image:pxg-10_map.png]]
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== '''pXG-20 Plasmid''' ==
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== '''PXG-20 Plasmid''' ==
 
[[Image:pxg-20_map.png]]
[[Image:pxg-20_map.png]]
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== '''pXG-30 Plasmid''' ==
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== '''PXG-30 Plasmid''' ==
 
[[Image:pxg-30.png]]
[[Image:pxg-30.png]]
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Latest revision as of 01:51, 19 July 2009

Click to go to the Illinois home page



Home
Team
Project
Experiments
Biobricks Notebook


Vectors

This page contains maps of the plasmids used in our project.

pJU-334 Plasmid

Pju-334 map.png

Note: The PZE12luc plasmid is the parental plasmid of the PJU-334 linear fragment.

pXG-10 Plasmid

Pxg-10 map.png

pXG-20 Plasmid

Pxg-20 map.png

pXG-30 Plasmid

Pxg-30.png

Questions about our Wiki page? Please email us at illinoisiGEM@gmail.com.

Retrieved from "http://2009.igem.org/Team:Illinois/Vectors"