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Indiana/6 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: Grow liquid culture to prepare for mini prep Preparation of Media: The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made...) |
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Grow liquid culture to prepare for mini prep | Grow liquid culture to prepare for mini prep | ||
| - | Preparation of Media | + | '''Preparation of Media''' |
The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made. | The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made. | ||
Kan media: | Kan media: | ||
| + | |||
50 mL LB | 50 mL LB | ||
| + | |||
50 uL KAN | 50 uL KAN | ||
Amp media: | Amp media: | ||
| + | |||
50 mL LB | 50 mL LB | ||
| + | |||
50 uL amp | 50 uL amp | ||
| + | |||
| + | |||
add 3 mL of media to a tube | add 3 mL of media to a tube | ||
| + | |||
transfer 1 colony into each tube | transfer 1 colony into each tube | ||
| + | |||
shake @ 37 C o/n | shake @ 37 C o/n | ||
Revision as of 19:06, 19 July 2009
Grow liquid culture to prepare for mini prep
Preparation of Media
The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made.
Kan media:
50 mL LB
50 uL KAN
Amp media:
50 mL LB
50 uL amp
add 3 mL of media to a tube
transfer 1 colony into each tube
shake @ 37 C o/n
