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Indiana/6 July 2009
From 2009.igem.org
(Difference between revisions)
(New page: Grow liquid culture to prepare for mini prep Preparation of Media: The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made...) |
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| - | Grow liquid culture to prepare for mini prep | + | '''Grow liquid culture to prepare for mini prep''' |
Preparation of Media: | Preparation of Media: | ||
The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made. | The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made. | ||
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Kan media: | Kan media: | ||
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50 mL LB | 50 mL LB | ||
| + | |||
50 uL KAN | 50 uL KAN | ||
| + | |||
| + | |||
Amp media: | Amp media: | ||
| + | |||
50 mL LB | 50 mL LB | ||
| + | |||
50 uL amp | 50 uL amp | ||
| + | |||
| + | |||
add 3 mL of media to a tube | add 3 mL of media to a tube | ||
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transfer 1 colony into each tube | transfer 1 colony into each tube | ||
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shake @ 37 C o/n | shake @ 37 C o/n | ||
Latest revision as of 19:07, 19 July 2009
Grow liquid culture to prepare for mini prep
Preparation of Media:
The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made.
Kan media:
50 mL LB
50 uL KAN
Amp media:
50 mL LB
50 uL amp
add 3 mL of media to a tube
transfer 1 colony into each tube
shake @ 37 C o/n
