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Indiana/6 July 2009
From 2009.igem.org
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The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made. | The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made. | ||
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Kan media: | Kan media: | ||
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50 uL KAN | 50 uL KAN | ||
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Amp media: | Amp media: | ||
Latest revision as of 19:07, 19 July 2009
Grow liquid culture to prepare for mini prep
Preparation of Media:
The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made.
Kan media:
50 mL LB
50 uL KAN
Amp media:
50 mL LB
50 uL amp
add 3 mL of media to a tube
transfer 1 colony into each tube
shake @ 37 C o/n
