"
Illinois/17 June 2009
From 2009.igem.org
(Difference between revisions)
(→June 17) |
|||
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{UIUC09Temp}} | {{UIUC09Temp}} | ||
{{Illinois}} | {{Illinois}} | ||
| + | <html> | ||
| + | <div id="uicontentbox"> | ||
| + | </html> | ||
{{IllinoisCalendar}} | {{IllinoisCalendar}} | ||
== June 17 == | == June 17 == | ||
| - | The four groups added restriction endonucleases to their synthesized DNA and | + | The four groups successfully added restriction endonucleases to their synthesized DNA and purified it by gel electrophoresis. The MicC gene was synthesized by PCR and verified to be successful by gel electrophoresis. |
[[Image:UI09MicCGel.jpg]] | [[Image:UI09MicCGel.jpg]] | ||
| + | |||
| + | We performed a PCR on the pJU-334 plasmid and ran a gel, which indicated a faint band at ~3.1kbp (the expected size of the plasmid) and a strong band at ~1.9kbp. | ||
| + | |||
| + | [[Image:Illinois-_Gel_3_of_pJU-334.jpg]] | ||
[https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page] | [https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page] | ||
| + | <html> | ||
| + | </div> | ||
| + | </html> | ||
| + | {{IllinoisBottomNav}} | ||
Latest revision as of 21:17, 20 July 2009
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
June 17
The four groups successfully added restriction endonucleases to their synthesized DNA and purified it by gel electrophoresis. The MicC gene was synthesized by PCR and verified to be successful by gel electrophoresis.
We performed a PCR on the pJU-334 plasmid and ran a gel, which indicated a faint band at ~3.1kbp (the expected size of the plasmid) and a strong band at ~1.9kbp.
