Illinois/17 June 2009

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We performed a PCR on the pJU-334 plasmid and ran a gel, which indicated a faint band at ~3.1kbp (the expected size of the plasmid) and a strong band at ~1.9kbp. 
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[[Image:Illinois-_Gel_3_of_pJU-334.jpg]]
[https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page]
[https://2009.igem.org/Team:Illinois/Notebook Back to Notebook page]
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Latest revision as of 21:17, 20 July 2009

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June
MTWTFSS
[http://2009.igem.org/Illinois/1_June_2009 1] [http://2009.igem.org/wiki/index.php?title=Illinois/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Illinois/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Illinois/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Illinois/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Illinois/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Illinois/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Illinois/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Illinois/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Illinois/14_June_2009&action=edit 14]
[http://2009.igem.org/Illinois/15_June_2009 15] [http://2009.igem.org/Illinois/16_June_2009 16] [http://2009.igem.org/Illinois/17_June_2009 17] [http://2009.igem.org/Illinois/18_June_2009 18] [http://2009.igem.org/Illinois/19_June_2009 19] [http://2009.igem.org/wiki/index.php?title=Illinois/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Illinois/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Illinois/22_June_2009&action=edit 22] [http://2009.igem.org/Illinois/23_June_2009 23] [http://2009.igem.org/Illinois/24_June_2009 24] [http://2009.igem.org/Illinois/25_June_2009 25] [http://2009.igem.org/Illinois/26_June_2009 26] [http://2009.igem.org/wiki/index.php?title=Illinois/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_June_2009&action=edit 28]
[http://2009.igem.org/Illinois/29_June_2009 29] [http://2009.igem.org/Illinois/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Illinois/1_July_2009 1] [http://2009.igem.org/Illinois/2_July_2009 2] [http://2009.igem.org/Illinois/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_July_2009&action=edit 5]
[http://2009.igem.org/Illinois/6_July_2009 6] [http://2009.igem.org/Illinois/7_July_2009 7] [http://2009.igem.org/wiki/index.php?title=Illinois/8_July_2009&action=edit 8] [http://2009.igem.org/Illinois/9_July_2009 9] [http://2009.igem.org/Illinois/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_July_2009&action=edit 12]
[http://2009.igem.org/Illinois/13_July_2009 13] [http://2009.igem.org/Illinois/14_July_2009 14] [http://2009.igem.org/Illinois/15_July_2009 15] [http://2009.igem.org/Illinois/16_July_2009 16] [http://2009.igem.org/Illinois/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Illinois/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Illinois/19_July_2009&action=edit 19]
[http://2009.igem.org/Illinois/20_July_2009 20] [http://2009.igem.org/Illinois/21_July_2009 21] [http://2009.igem.org/Illinois/22_July_2009 22] [http://2009.igem.org/Illinois/23_July_2009 23] [http://2009.igem.org/Illinois/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Illinois/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Illinois/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Illinois/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Illinois/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Illinois/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Illinois/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Illinois/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Illinois/2_August_2009&action=edit 2]
[http://2009.igem.org/wiki/index.php?title=Illinois/3_August_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_August_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_August_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Illinois/6_August_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Illinois/7_August_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Illinois/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Illinois/9_August_2009&action=edit 9]
[http://2009.igem.org/wiki/index.php?title=Illinois/10_August_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_August_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_August_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Illinois/13_August_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Illinois/14_August_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Illinois/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Illinois/16_August_2009&action=edit 16]
[http://2009.igem.org/wiki/index.php?title=Illinois/17_August_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Illinois/18_August_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Illinois/19_August_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Illinois/20_August_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Illinois/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Illinois/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Illinois/23_August_2009&action=edit 23]
[http://2009.igem.org/wiki/index.php?title=Illinois/24_August_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Illinois/25_August_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Illinois/26_August_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Illinois/27_August_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_August_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Illinois/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Illinois/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=Illinois/31_August_2009&action=edit 31]

June 17

The four groups successfully added restriction endonucleases to their synthesized DNA and purified it by gel electrophoresis. The MicC gene was synthesized by PCR and verified to be successful by gel electrophoresis.

UI09MicCGel.jpg

We performed a PCR on the pJU-334 plasmid and ran a gel, which indicated a faint band at ~3.1kbp (the expected size of the plasmid) and a strong band at ~1.9kbp.

Illinois- Gel 3 of pJU-334.jpg


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