Week3

From 2009.igem.org

(Difference between revisions)
Line 1: Line 1:
1.M9 media preparation
1.M9 media preparation
-
2.IPTG stock preparation
+
2.1M IPTG stock  
3.First FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: blank contaminated)
3.First FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: blank contaminated)
4.Second FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: sample stored after pelleting)
4.Second FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: sample stored after pelleting)

Revision as of 09:33, 22 July 2009

1.M9 media preparation

2.1M IPTG stock

3.First FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: blank contaminated)

4.Second FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: sample stored after pelleting)

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