Virginia Commonwealth/22 July 2009

From 2009.igem.org

(Difference between revisions)
(New page: ==Wednesday 22 July 2009== ===Results=== * Overnight cultures were successful ** Vials had noticeable amounts of white E. coli at the bottom of the tube. * Flow Cytometer was repaired ** S...)
(→Wednesday 22 July 2009)
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====Wetlab====
====Wetlab====
* Samples from cryogenic stocks were grown up in LB + Cm:
* Samples from cryogenic stocks were grown up in LB + Cm:
-
** J23101
+
** J23101 w/ E0240
-
** J23101
+
** J23101 w/ J06702
-
** J23102
+
** J23102 w/ E0240
-
** J23102
+
** J23102 w/ J06702
-
** J23103
+
** J23103 w/ E0240
-
** J23103
+
** J23103 w/ J06702
-
** J23104
+
** J23104 w/ E0240
-
** J23104
+
** J23104 w/ J06702
-
** J23105
+
** J23105 w/ E0240
-
 
+
** J23105 w/ J06702
 +
** J23106 w/ E0240
 +
** J23106 w/ J06702
 +
** J23107 w/ E0240
 +
** J23107 w/ J06702
** J23110 w/ E0240
** J23110 w/ E0240
** J23110 w/ J06702
** J23110 w/ J06702
Line 43: Line 47:
** R0040 w/ E0240
** R0040 w/ E0240
** R0040 w/ J06702
** R0040 w/ J06702
 +
* Samples from cryogenic stocks were grown up in LB:
 +
** DB 3.1 (2 vials)
 +
* Samples from cryogenic stocks were grown up in LB + Amp:
 +
** pSB4C5

Revision as of 20:02, 22 July 2009

Contents

Wednesday 22 July 2009

Results

  • Overnight cultures were successful
    • Vials had noticeable amounts of white E. coli at the bottom of the tube.
  • Flow Cytometer was repaired
    • Samples cannot be tested until protocols for using and maintaining the machine are figured out
  • Plated bacteria are still not expressing GFP and RFP

Trentay

Tasks

  • Dilute E. coli into fresh LB + Cm in preparation for testing with the flow cytometer
    • nothing was tested today with the cytometer
  • Grow all BioBrick parts in 5 mL of LB + Cm overnight
    • 100 microliters was added to broth
    • plastic tubes had tops, so tops were loosened during the incubation period
    • cells were out of the -80 degree Celsius freezer for a prolonged amount of time, so viability is lower than usual.
      • If nothing grows, BioBrick DNA will be reinserted into fresh NEB 10 beta through electrotransformation.
  • Organize notebook and running draft

Trentay 19:57, 22 July 2009 (UTC)

  • Pick colonies from transformation of J23100 and J23106 with RET E0240

Wetlab

  • Samples from cryogenic stocks were grown up in LB + Cm:
    • J23101 w/ E0240
    • J23101 w/ J06702
    • J23102 w/ E0240
    • J23102 w/ J06702
    • J23103 w/ E0240
    • J23103 w/ J06702
    • J23104 w/ E0240
    • J23104 w/ J06702
    • J23105 w/ E0240
    • J23105 w/ J06702
    • J23106 w/ E0240
    • J23106 w/ J06702
    • J23107 w/ E0240
    • J23107 w/ J06702
    • J23110 w/ E0240
    • J23110 w/ J06702
    • J23113 w/ E0240
    • J23113 w/ J06702
    • J23116 w/ E0240
    • J23116 w/ J06702
    • R0011 w/ E0240
    • R0011 w/ J06702
    • R0040 w/ E0240
    • R0040 w/ J06702
  • Samples from cryogenic stocks were grown up in LB:
    • DB 3.1 (2 vials)
  • Samples from cryogenic stocks were grown up in LB + Amp:
    • pSB4C5
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