Team:Paris/Protocols

From 2009.igem.org

(Difference between revisions)
(Protocols)
Line 10: Line 10:
----
----
-
iGEM paris 2007 protocols link :  
+
iGEM paris 2009 protocols link :  
----
----
-
[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]
 
-
 
+
<html>
-
<u>content :</u>
+
</div>
-
 
+
<div id="paris_content_boxtop">
-
*Growing_bacteria_in_liquid_medium
+
</div>
-
*Preparing growth media
+
<div id="paris_content">
-
**exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
+
</html>
-
*Chemical transformation
+
-
*Glycerol Stock
+
-
*Recombineering/Lambda red-mediated gene replacement
+
-
*Miniprep
+
-
*Fluorescent single cells visualisation
+
-
*Digestion reactions
+
Line 47: Line 40:
*PCR Screening
*PCR Screening
*Protocol to make competent bacteria
*Protocol to make competent bacteria
 +
----
----
-
iGEM paris 2009 protocols link :  
+
iGEM paris 2007 protocols link :  
----
----
 +
[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]
-
<html>
+
 
-
</div>
+
<u>content :</u>
-
<div id="paris_content_boxtop">
+
 
-
</div>
+
*Growing_bacteria_in_liquid_medium
-
<div id="paris_content">
+
*Preparing growth media
-
</html>
+
**exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
 +
*Chemical transformation
 +
*Glycerol Stock
 +
*Recombineering/Lambda red-mediated gene replacement
 +
*Miniprep
 +
*Fluorescent single cells visualisation
 +
*Digestion reactions
== Protocols suggested ==
== Protocols suggested ==

Revision as of 10:59, 23 July 2009

Protocols

Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . We try our best not to make it redondant.



iGEM paris 2009 protocols link :





iGEM paris 2008 protocols link :



[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]


content :

  • Electrophoresis
  • Concentration of the Miniprep or the Midiprep
  • Amplification of promoters.
  • PCR Screening
  • Protocol to make competent bacteria



iGEM paris 2007 protocols link :



[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]


content :

  • Growing_bacteria_in_liquid_medium
  • Preparing growth media
    • exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
  • Chemical transformation
  • Glycerol Stock
  • Recombineering/Lambda red-mediated gene replacement
  • Miniprep
  • Fluorescent single cells visualisation
  • Digestion reactions

Protocols suggested

ahah