Team:Paris/Protocols
From 2009.igem.org
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*PCR Screening | *PCR Screening | ||
*Protocol to make competent bacteria | *Protocol to make competent bacteria | ||
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- | iGEM paris | + | iGEM paris 2007 protocols link : |
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+ | [http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here] | ||
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- | </ | + | <u>content :</u> |
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- | + | *Growing_bacteria_in_liquid_medium | |
- | + | *Preparing growth media | |
- | + | **exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel | |
+ | *Chemical transformation | ||
+ | *Glycerol Stock | ||
+ | *Recombineering/Lambda red-mediated gene replacement | ||
+ | *Miniprep | ||
+ | *Fluorescent single cells visualisation | ||
+ | *Digestion reactions | ||
== Protocols suggested == | == Protocols suggested == |
Revision as of 10:59, 23 July 2009
Protocols
Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . We try our best not to make it redondant.
iGEM paris 2009 protocols link :
iGEM paris 2008 protocols link :
[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]
content :
- Electrophoresis
- Concentration of the Miniprep or the Midiprep
- Amplification of promoters.
- PCR Screening
- Protocol to make competent bacteria
iGEM paris 2007 protocols link :
[http://parts.mit.edu/igem07/index.php/Paris/PROTOCOLS Click here]
content :
- Growing_bacteria_in_liquid_medium
- Preparing growth media
- exemple: Making 10 petri dish (LB+erythromycin+citrate+DAP), Solid M9 Minimum Medium, preparation of agarosis gel
- Chemical transformation
- Glycerol Stock
- Recombineering/Lambda red-mediated gene replacement
- Miniprep
- Fluorescent single cells visualisation
- Digestion reactions
Protocols suggested
ahah