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Virginia Commonwealth/23 July 2009
From 2009.igem.org
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* Make cryogenic stocks of all overnight cultures | * Make cryogenic stocks of all overnight cultures | ||
* Make stocks of parts I1352 (RFP) and I13522 (GFP) | * Make stocks of parts I1352 (RFP) and I13522 (GFP) | ||
| - | * Update | + | * Update notebook, documents |
| + | * Pick colonies from transformation | ||
| + | ** pick controls as well to make more seed and electrocompotent stock | ||
| + | [[User:Trentay|Trentay]] 17:25, 23 July 2009 (UTC) | ||
---- | ---- | ||
====Wetlab==== | ====Wetlab==== | ||
''Maria and Afton'' | ''Maria and Afton'' | ||
| - | * | + | * Cryogenic stocks were made |
| + | [[User:Trentay|Trentay]] 17:25, 23 July 2009 (UTC) | ||
Revision as of 17:25, 23 July 2009
Contents |
Thursday 23 July 2009
Results
Maria and Afton
- All overnight cultures grew except one vial of NEB 10 beta seed stock
- there was a second vial of seed stock that did grow successfully
- Transformations were successful
| Name | Type | Plate | Purpose | Growth Observation | |
|---|---|---|---|---|---|
| NEB 10 beta | seed stock | LB | make more seed stock | lawn | |
| NEB 10 beta | electro comp. | LB | (+) Control/ make more stock | lawn | |
| NEB 10 beta | electro comp. | LB | (+) Control shocked | lawn | |
| DB 3.1 | electro comp. | LB | (+) Control shocked | lawn | |
| DB 3.1 | electro comp. | LB | (+) Control/make more stock | lawn | |
| NEB 10 beta | electro comp. | Cm | (-) Control | no growth | |
| NEB 10 beta | electro comp. | Cm | J23100 w/ J06702 | 6 small colonies |
Trentay 17:19, 23 July 2009 (UTC)
Tasks
Maria and Afton
- Make cryogenic stocks of all overnight cultures
- Make stocks of parts I1352 (RFP) and I13522 (GFP)
- Update notebook, documents
- Pick colonies from transformation
- pick controls as well to make more seed and electrocompotent stock
Trentay 17:25, 23 July 2009 (UTC)
Wetlab
Maria and Afton
- Cryogenic stocks were made
Trentay 17:25, 23 July 2009 (UTC)
