Team:KULeuven/24 July 2009

From 2009.igem.org

(Difference between revisions)
(→Vanillin Receptor)
(→Progress)
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[[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-3W269GC-2C&_user=877992&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000047079&_version=1&_urlVersion=0&_userid=877992&md5=95a48c4a2e3cf93948ab45c9a92e78a6 3]]Yong-Woog Lee, Shouguang Jin, Woong-Seop Sim, Eugene W. Nester, The sensing of plant signal molecules by Agrobacterium: genetic evidence for direct recognition of phenolic inducers by the VirA protein, Gene, Volume 179, Issue 1, 1996, Pages 83-88, ISSN 0378-1119, DOI: 10.1016/S0378-1119(96)00328-9. <br>
[[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-3W269GC-2C&_user=877992&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000047079&_version=1&_urlVersion=0&_userid=877992&md5=95a48c4a2e3cf93948ab45c9a92e78a6 3]]Yong-Woog Lee, Shouguang Jin, Woong-Seop Sim, Eugene W. Nester, The sensing of plant signal molecules by Agrobacterium: genetic evidence for direct recognition of phenolic inducers by the VirA protein, Gene, Volume 179, Issue 1, 1996, Pages 83-88, ISSN 0378-1119, DOI: 10.1016/S0378-1119(96)00328-9. <br>
[[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=40333 4]]Y W Lee, S Jin, W S Sim, and E W Nester,Genetic evidence for direct sensing of phenolic compounds by the VirA protein of Agrobacterium tumefaciens, Proc Natl Acad Sci U S A. 1995 December 19; 92(26): 12245–12249.<br>
[[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=40333 4]]Y W Lee, S Jin, W S Sim, and E W Nester,Genetic evidence for direct sensing of phenolic compounds by the VirA protein of Agrobacterium tumefaciens, Proc Natl Acad Sci U S A. 1995 December 19; 92(26): 12245–12249.<br>
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==Vanillin synthesis==
 +
Received mail from U. Edinburgh:
 +
We will receive:
 +
* Sam5; no part number, Pst1 site removed
 +
* Sam8; coding sequence without RBS {{kulpart|BBa_I742142}}
 +
* ComT; coding sequence without RBS {{kulpart|BBa_I742107}}
 +
* ech; RBS + ech, in pSB1A2, {{kulpart|BBa_I742113}}
 +
* fcs; RBS + fcs, in pSB1A2, {{kulpart|BBa_I742115}}

Revision as of 11:39, 24 July 2009

Progress

Vanillin Receptor

Possible receptor is the VirA/VirG system from Agrobacterium, the VirA protein will bind to phenolic compounds and induce transcription of vir genes by phosphorylation of VirG. protein ChvE results in a broader response to phenolic compounds. papers:

http://mmbr.asm.org/cgi/content/abstract/56/1/12 1 S C Winans, Two-way chemical signaling in Agrobacterium-plant interactions, Microbiol Mol Biol Rev. 1992 March; 56(1): 12-31
http://jb.asm.org/cgi/content/abstract/180/21/5632 2 Wen-Tao Peng, Yong-Woog Lee, and Eugene W. Nester, The Phenolic Recognition Profiles of the Agrobacterium tumefaciens VirA Protein Are Broadened by a High Level of the Sugar Binding Protein ChvE, Journal of Bacteriology, November 1998, p. 5632-5638, Vol. 180, No. 21
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-3W269GC-2C&_user=877992&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000047079&_version=1&_urlVersion=0&_userid=877992&md5=95a48c4a2e3cf93948ab45c9a92e78a6 3Yong-Woog Lee, Shouguang Jin, Woong-Seop Sim, Eugene W. Nester, The sensing of plant signal molecules by Agrobacterium: genetic evidence for direct recognition of phenolic inducers by the VirA protein, Gene, Volume 179, Issue 1, 1996, Pages 83-88, ISSN 0378-1119, DOI: 10.1016/S0378-1119(96)00328-9.
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=40333 4Y W Lee, S Jin, W S Sim, and E W Nester,Genetic evidence for direct sensing of phenolic compounds by the VirA protein of Agrobacterium tumefaciens, Proc Natl Acad Sci U S A. 1995 December 19; 92(26): 12245–12249.

Vanillin synthesis

Received mail from U. Edinburgh: We will receive:

  • Sam5; no part number, Pst1 site removed
  • Sam8; coding sequence without RBS
  • ComT; coding sequence without RBS
  • ech; RBS + ech, in pSB1A2,
  • fcs; RBS + fcs, in pSB1A2,
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