Virginia Commonwealth/16 July 2009
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* Transformation of NEB 10 beta to make sure new stock is electrocompetent | * Transformation of NEB 10 beta to make sure new stock is electrocompetent | ||
[[User:Trentay|Trentay]] 20:41, 16 July 2009 (UTC) | [[User:Trentay|Trentay]] 20:41, 16 July 2009 (UTC) | ||
+ | |||
+ | ''Craig and Clay'' | ||
+ | * The registry will be contacted to verify the correct resistence for the limonene synthesis related parts. New DNA will also be requested. | ||
---- | ---- | ||
Revision as of 14:15, 24 July 2009
Contents |
Thursday 16 July 2009
Results
- Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation
Promoter | Reporter | Resistance/Plate | Growth Observation | |
---|---|---|---|---|
J23101 | E0240 | Cm | no growth | |
J23101 | J06702 | Cm | 21 colonies | |
J23102 | E0240 | Cm | 3 colonies | |
J23102 | J06702 | Cm | 8 colonies | |
J23103 | E0240 | Cm | 2 colonies | |
J23103 | J06702 | Cm | 23 colonies | |
J23104 | E0240 | Cm | 9 colonies | |
J23104 | J06702 | Cm | 12 colonies | |
J23105 | E0240 | Cm | no growth | |
J23105 | J06702 | Cm | 14 colonies | |
J23107 | E0240 | Cm | 3 colonies | |
J23107 | J06702 | Cm | 3 colonies |
- No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
- Vial may have thawed out too much after cells were taken out for the electrocompetency procedure
Trentay 14:31, 16 July 2009 (UTC)
--Craig and Clay--
- No growth was seen on either the kanamycin or chloramphenicol plates.
- The sequence for the LIM1 gene was verified to be optimized for E. coli.
Tasks
Maria and Afton
- Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
- Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
- Run an electrophoresis test on promoters and reporter Eo240
- Pick colonies of promoters from transformation 100-107 (excluding 106)
- Transformation of NEB 10 beta to make sure new stock is electrocompetent
Trentay 20:41, 16 July 2009 (UTC)
Craig and Clay
- The registry will be contacted to verify the correct resistence for the limonene synthesis related parts. New DNA will also be requested.
Wetlab
Maria and Afton
- Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
- Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
- Run an electrophoresis test on promoters and reporter Eo240
Trentay 20:41, 16 July 2009 (UTC)